Genotyping and Phytochemical Analysis of Kayu Pule Plant as Local Bali Medicinal Plant

I Ketut Suada; I Gede Putu Wirawan; I Nyoman Wijaya; Anak Agung Sagung Intan Gayatri; Gusti Ayu Putu Tiara Adi Hantari; Maria Malida Vernandes Sasadara; Putu Krisnawan Kalimutu; I Made Oka Parwata

doi:10.22146/jtbb.13086

I Ketut Suada Faculty of Agriculture, Udayana University, Jalan P.B. Sudirman, Denpasar, Bali 80239 https://orcid.org/0000-0003-2362-4345
I Gede Putu Wirawan Faculty of Agriculture, Udayana University, Jalan P.B. Sudirman, Denpasar, Bali 80239 https://orcid.org/0000-0002-5371-8185
I Nyoman Wijaya Faculty of Agriculture, Udayana University, Jalan P.B. Sudirman, Denpasar, Bali 80239
Anak Agung Sagung Intan Gayatri Faculty of Agriculture, Udayana University, Jalan P.B. Sudirman, Denpasar, Bali 80239
Gusti Ayu Putu Tiara Adi Hantari Faculty of Agriculture, Udayana University, Jalan P.B. Sudirman, Denpasar, Bali 80239
Maria Malida Vernandes Sasadara Faculty of Pharmacy, Universitas Mahasaraswati Denpasar, Jl. Kamboja No.11A, Dangin Puri Kangin, Denpasar Utara, Kota Denpasar, Bali 80233 https://orcid.org/0000-0002-5148-2981
Putu Krisnawan Kalimutu Faculty of Agriculture, Udayana University, Jalan P.B. Sudirman, Denpasar, Bali 80239
I Made Oka Parwata Bali Provincial Office of Agriculture and Food Security Jalan Raya W.R. Supratman no. 71 Denpasar, Bali 80114

DOI: https://doi.org/10.22146/jtbb.13086

Keywords: Alstonia scolaris, Genotyping, Kayu Pule, matK, Phytochemical compounds

Abstract

The bark of Kayu Pule plants in Bali is empirically known as a traditional medicinal ingredient and has been developed as a cosmetic and other health ingredient; however, scientific research has yet to be conducted on the profiles of the plant. This study aimed to determine the plant species, examine the scientific function of the compounds, and the antioxidant activity of the plant's ethanolic extract. This study performed a DNA analysis of the plant using matK primer, and the amplified DNA sequences were used to determine the phylogenetic tree. Based on the molecular analysis, the Kayu Pule plant bark from Bali, which was used as medicine, was Alstonia scholaris. The main compounds in Kayu Pule bark, such as ergost-5-en-3-ol and 12-oleanen-3-yl acetate, had anti-inflammatory, antioxidant, and antimicrobial properties. The antioxidant strength of the Kayu Pule plant was measured with IC50 of 3.7 μg mL^-1 with a very strong category. This research showed the potential of Kayu Pule for developing medicinal and cosmetic products.

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