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Vol 16 No 2 (2022): September

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Submitted
Jul 28, 2021
Accepted
Jul 18, 2022
Published
Sep 12, 2022
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Effect of Several Solvents on the Chemical Compound, Antioxidant Activity, and Cytotoxicity Levels of Acacia decurrens Willd. Gum Extract

https://doi.org/10.22146/jik.v16i2.2254
Ramadhany Ayu Purnama
Ramadhani1u@gmail.com
Universitas Gadjah Mada
Sigit Sunarta
Universitas Gadjah Mada
Hilda Ismail
Universitas Gadjah Mada

Corresponding Author(s) : Ramadhany Ayu Purnama

Ramadhani1u@gmail.com

Jurnal Ilmu Kehutanan, Vol 16 No 2 (2022): September

Abstract

This study aimed to determine the chemical compounds in A. decurrens gum and its bioactivity potential. Extraction was carried out in multilevel with the reflux technique using n-hexane, ethanol, and water as solvents. The chemical compounds were identified using Gas Chromatography-Mass Spectrometry (GC-MS). The amount of A. decurrens gum extract yield, total phenolic content (TPC), total flavonoid content (TFC), and total flavonols content (TVC) were then analyzed quantitatively. Furthermore, bioactivity in the form of antioxidant activity was analyzed with the 2,2-diphenyl-1-picrylhydrazil (DPPH) method, while the cytotoxicity level was assessed using the brine shrimp lethality assay (BSLT). The GC-MS analysis showed that the n-hexane-soluble fraction contained alkane, alkene, aldehyde, ester, and aromatics, while the ethanol-soluble fraction contained monosaccharide and fat group compounds. The water-soluble fraction contained only the monosaccharide group. The highest content value was obtained from the ethanol-soluble fraction, namely TPC, TFC, TVC, and antioxidant activity of 19.61±0.47 mgGA/g, 23.89±1.32 mgQE/g, 13.41±0.81 mgCE/g, and IC50: 3.842 g/mL, respectively. The cytotoxicity content obtained from each fraction sequentially was 0.47 ppm, 5.91 ppm, and 6.56 ppm for n-hexane, ethanol, and water, respectively. 

Keywords

multilevel extraction TPC TFC TVC bioactivity
Ayu Purnama, R., Sunarta, S., & Ismail, H. (2022). Effect of Several Solvents on the Chemical Compound, Antioxidant Activity, and Cytotoxicity Levels of Acacia decurrens Willd. Gum Extract. Jurnal Ilmu Kehutanan, 16(2), 171-183. https://doi.org/10.22146/jik.v16i2.2254
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Author Biographies

Ramadhany Ayu Purnama, Universitas Gadjah Mada

Faculty of Forestry, Universitas Gadjah Mada, Yogjakarta, 55281

Sigit Sunarta, Universitas Gadjah Mada

Faculty of Forestry, Universitas Gadjah Mada, Yogjakarta, 55281

Hilda Ismail, Universitas Gadjah Mada

Faculty of Pharmacy, Universitas Gajdah Mada, Yogyakarta, 55281

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