Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats
Keywords:
A. paniculata, C. asiatica, Antioxidant, Antidiabetes, Pancreatic Protection
Abstract
Many medicinal plants are widely grown in South- and Southeast Asia countries. Some of them are traditionally used for treatment of diabetes mellitus such as Andrographis paniculata and Centella asiatica. In the study, we provided fractionated-extracts of A. paniculata and C. asiatica to increase the concentration of their active compounds and eliminate unexpected substances. The aim of the study was to evaluate the antidiabetes effect of the combination of their fractionated-extracts in male neonatal streptozotocin (STZ)-induced diabetic rats. In the study, diabetes was injected intraperitoneally 90mg.kg-1 BWSTZ to two day-old rats. At the age of three months, the rats were administered with the combination of both fractionated-extracts for 14 consecutive days. We evaluated antidiabetes with parameters of blood glucose levels, morphology of pancreatic islet, β-cell density as well as immunohistochemical pancreatic insulin. The levels of MDA, SOD and GPx were also determined about oxidative stress change after treatment with the combination. In the study, the combination succeeded to lower the blood glucose level in neonatal STZ-induced diabetic rats. Inline with fact, improvement of rat pancreatic islets and β cells density, as well as moderate restoration of pancreatic insulin, were observed after treatment with the combination. The substance could decrease the level of MDA, and increase the levels of SOD and GPx. In conclusion, the combination of fractionated-extracts of A. paniculata and C. asiatica exhibited potential antidiabetic effect to its antioxidative effect in male neonatal STZ-induced diabetes rats.
References
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Sari KRP, Sudarsono, Nugroho AE, 2015, Effect of herbal combination of Andrographispaniculata (Burm.f) Ness and Gynuraprocumbens (Lour.) Merrethanolic extracts in alloxan-induced hyperglycemic rats, Int. Food Res. J., 22(4), 1332-1337.
Schacht, V. and Kern, J.S., 2015.Basics of immunohistochemistry.The Journal of investigative dermatology, 135(3), p.e30
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Szkudelski, T., 2001. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol. Res. 50, 537–546.
Wasowicz, W., Neve, J. and Peretz, A., 1993. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clinical chemistry, 39(12), pp.2522-2526.
Weir, G.C., Clore, E.T., Zmachinski, C.J. and Bonner-Weir, S., 1981.Islet secretion in a new experimental model for non-insulin-dependent diabetes.Diabetes, 30(7), pp.590-595.
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Yin, Z., Yu, H., Chen, S., Ma, C., Ma, X., Xu, L., Ma, Z., Qu, R., Ma, S., 2015.Asiaticoside attenuates diabetes-induced cognition deficits by regulating PI3K/Akt/NF-κB pathway. Behav. Brain Res. 292, 288–299. doi:10.1016/j.bbr.2015.06.024
Zhang, X.-F.dan Tan, B.K.-H., 2000. AntihyperglycaemicAnd Anti‐Oxidant Properties Of AndrographisPaniculata In Normal And Diabetic Rats. Clinical and Experimental Pharmacology and Physiology, 27: 358–363.
Zhang, Z., Jiang, J., Yu, P., Zeng, X., Larrick, J.W., Wang, Y., 2009. Hypoglycemic and beta cell protective effects of andrographolide analogue for diabetes treatment.J. Transl. Med. 7, 62.doi:10.1186/1479-5876-7-62
Boden, G., Shulman, G.I., 2002. Free fatty acids in obesity and type 2 diabetes: defining their role in the development of insulin resistance and β-cell dysfunction. Eur. J. Clin. Invest. 32, 14–23.
Bonner-Weir, S., Trent, D.F., Honey, R.N. and Weir, G.C., 1981. Responses of neonatal rat islets to streptozotocin: limited B-cell regeneration and hyperglycemia. Diabetes, 30(1), pp.64-69.
Butler, A.E., Janson, J., Bonner-Weir, S., Ritzel, R., Rizza, R.A., dan Butler, P.C., 2003.β-cell deficit and increased β-cell apoptosis in humans with type 2 diabetes. Diabetes, 52: 102–110.
Chauhan, P.K., Pandey, I.P., Dhatwalia, V.K., 2010. Evaluation of the anti-diabetic effect of ethanolic and methanolic extracts of Centellaasiatica leaves extract on alloxan induced diabetic rats. AdvBiol Res 4, 27–30.
Centers for Disease Control and Prevention, 2011. National diabetes fact sheet: national estimates and general information on diabetes and prediabetes in the United States, 2011. Atlanta, GA: US department of health and human services, centers for disease control and prevention, 201(1), pp.2568-2569.
Evans, J.L., Goldfine, I.D., Maddux, B.A., Grodsky, G.M., 2003. Are oxidative stress- activated signaling pathways mediators of insulin resistance and β-cell dysfunction? Diabetes 52, 1–8.
Gayathri, V., Lekshmi, P., Padmanabhan, R., others, 2011. Anti-diabetes activity of ethanol extract of Centellaasiatica (L.) Urban (whole plant) in Streptozotocin-induced diabetic rats, isolation of an active fraction and toxicity evaluation of the extract. Int. J. Med. Aromat. Plants 1, 278–286.
Ismail, N.A., Okasha, S.H., Dhawan, A., Rahman, A.M.A., Hamid, N.A. and Shaker, O., 2012. Glutathione peroxidase, superoxide dismutase and catalase activities in children with chronic hepatitis.Advances in Bioscience and Biotechnology, 3(7):972-977.
Ji, X., Li, C., Ou, Y., Li, N., Yuan, K., Yang, G., Chen, X., Yang, Z., Liu, B., Cheung, W.W., Wang, L., Huang, R., Lan, T., 2016. Andrographolide ameliorates diabetic nephropathy by attenuating hyperglycemia-mediated renal oxidative stress and inflammation via Akt/NF-κB pathway. Mol. Cell. Endocrinol. 437, 268–279. doi:10.1016/j.mce.2016.06.029
Kikui, Y., Seguchi, H. and Mizoguti, H., 1977. A differential staining method for A-and B-cells in the pancreatic islets of Langerhans.ActaHistochemicaetCytochemica, 10(1), pp.10-13.
Lenzen, S., 2008.The mechanisms of alloxan- and streptozotocin-induced diabetes.Diabetologia 51, 216–226. doi:10.1007/s00125-007-0886-7
Lindawati, N.Y., Nugroho, A.E., Pramono, S, 2014.The Effect of Combination from Purified Extract of Sambiloto Herb [AndrographisPaniculata (Burm.F.)Nees]and Pegagan Herb [CentellaAsiatica (L.) Urban]of Translocation of GLUT-4 Protein In Type 2 Diabetes Mellitus-Insulin Resistance Rats. Traditional Medicine Journal, 19(2), 62-69.
Liu, J., He, T., Lu, Q., Shang, J., Sun, H. and Zhang, L., 2010. Asiatic acid preserves beta cell mass and mitigates hyperglycemia in streptozocin‐induced diabetic rats. Diabetes/metabolism research and reviews, 26(6), pp.448-454.
Maechler, P., Jornot, L., Wollheim, C.B., 1999. Hydrogen Peroxide Alters Mitochondrial Activation and Insulin Secretion in Pancreatic Beta Cells. J. Biol. Chem. 274, 27905–27913.
Nugroho, A.E., 2006. Animal Models of Diabetes Mellitus : Pathology and Mechanism of Some Diabetogenics. Biodiversitas J. Biol. Divers. 7, 378–382. doi:10.13057/biodiv/d070415
Nugroho, A.E., Rais, I.R., Setiawan, I., Pratiwi, P.Y., Hadibarata, T., Tegar, M., Pramono, S., 2013a. Pancreatic effect of andrographolide isolated from Andrographispaniculata (Burm. f.) Nees. Pak. J. Biol. Sci. 17, 22-31.
Nugroho AE. Lindawati NY, Herlyanti K, Widyastuti L, Pramono S, 2013b, Anti-diabetic effect of a combination of andrographolide-enriched extract of andrographispaniculata (Burm f.) Nees and asiaticoside-enriched extract of Centellaasiatica L. in high fructose-fat fed rats, Indian J. Exp. Biol., 51(12), 1101-1108.
Nugroho AE., Kusumaramdani G, Widyaninggar A, Anggoro DP, Pramono S, 2014a, Antidiabetic effect of combinations of n-hexane insoluble fraction of ethanolic extract of Andrographispaniculata with other traditional medicines, Int. Food Res. J., 21(2), 785-789.
Nugroho AE., Purnomo Sari KR, Sunarwidhi AL, Sudarsono, 2014b, Blood glucose reduction by combination of Andrographispaniculata (Burm. f.) Ness herbs and Azadirachtaindica A. Juss leaves in alloxan-induced diabetic rats, J. Appl. Pharm. Sci., 4(9), 30-35.
Ramanathan, M., Jaiswal, A.K. and Bhattacharya, S.K., 1999. Superoxide dismutase, catalase and glutathione peroxidase activities in the brain of streptozotocin induced diabetic rats.
Rizvi, R.Y., Rahman, A. and Hadi, S.M., 1986. Effect of alkylation with streptozotocin on the secondary structure of DNA.Bioscience Reports, 6(6), pp.557-564.
Sari KRP, Sudarsono, Nugroho AE, 2015, Effect of herbal combination of Andrographispaniculata (Burm.f) Ness and Gynuraprocumbens (Lour.) Merrethanolic extracts in alloxan-induced hyperglycemic rats, Int. Food Res. J., 22(4), 1332-1337.
Schacht, V. and Kern, J.S., 2015.Basics of immunohistochemistry.The Journal of investigative dermatology, 135(3), p.e30
Subramanian, R., Asmawi, M.Z., Sadikun, A., 2008. In vitro alpha-glucosidase and alpha-amylase enzyme inhibitory effects of Andrographispaniculata extract and andrographolide.ActaBiochim Pol 55, 391–398.
Szkudelski, T., 2001. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol. Res. 50, 537–546.
Wasowicz, W., Neve, J. and Peretz, A., 1993. Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage. Clinical chemistry, 39(12), pp.2522-2526.
Weir, G.C., Clore, E.T., Zmachinski, C.J. and Bonner-Weir, S., 1981.Islet secretion in a new experimental model for non-insulin-dependent diabetes.Diabetes, 30(7), pp.590-595.
World Health Organization, 2016.World Health Day 2016 – How to get involved, Singapore, WHO.
Yin, Z., Yu, H., Chen, S., Ma, C., Ma, X., Xu, L., Ma, Z., Qu, R., Ma, S., 2015.Asiaticoside attenuates diabetes-induced cognition deficits by regulating PI3K/Akt/NF-κB pathway. Behav. Brain Res. 292, 288–299. doi:10.1016/j.bbr.2015.06.024
Zhang, X.-F.dan Tan, B.K.-H., 2000. AntihyperglycaemicAnd Anti‐Oxidant Properties Of AndrographisPaniculata In Normal And Diabetic Rats. Clinical and Experimental Pharmacology and Physiology, 27: 358–363.
Zhang, Z., Jiang, J., Yu, P., Zeng, X., Larrick, J.W., Wang, Y., 2009. Hypoglycemic and beta cell protective effects of andrographolide analogue for diabetes treatment.J. Transl. Med. 7, 62.doi:10.1186/1479-5876-7-62
Published
2020-12-30
How to Cite
Ariastuti, R., Fitrawan, L. O. M., Nugroho, A. E., & Pramono, S. (2020). Antidiabetes of Combination of Fractionated-extracts of Andrographis paniculata and Centella asiatica in Neonatal Streptozotocin-induced Diabetic Rats. Indonesian Journal of Pharmacy, 31(4), 312-322. https://doi.org/10.22146/ijp.1135
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