Eleutherine palmifolia (L.) Merr. Extract Increases The Crypts and Caspase-3 Expression in Colitis-Associated Colon Cancer Model

Roihatul  Mutiah; Wahyi Yucha  Firsyaradha; Riza Ambar  Sari; Rahmi  Annisa; Risma Aprinda  Kristanti; Yen Yen Ari Indrawijaya; Tias Pramesti  Griana; Anik  Listiyana

doi:10.22146/ijp.1120

Roihatul Mutiah Department of Pharmacy, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144
Wahyi Yucha Firsyaradha Department of Pharmacy, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144
Riza Ambar Sari Department of Pharmacy, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144
Rahmi Annisa Department of Pharmacy, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144
Risma Aprinda Kristanti Department of Biomedic, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144
Yen Yen Ari Indrawijaya Department of Pharmacy, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144
Tias Pramesti Griana Department of Biomedic, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144
Anik Listiyana Department of Biomedic, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144

Keywords: Eleutherine palmifolia extract, Colitis-Associated Colon Cancer, Colonic histopathology, Caspase-3

Abstract

It is known that Eleutherine palmifolia (L.) Merr contains flavonoid and polyphenol as bioactive compounds that have the ability as chemopreventive agents. This study aims to examine the effect of Eleutherine palmifolia (L.) Merr extract (EPE) on colon histopathology and the enhancement of caspase-3 expression in BALB/c mice of colitis-associated colon cancer (CAC) model. Thirty Balb/c female mice were used in this study and were divided into six groups. Five mice in one group were normal or negative control (given phosphate-buffered saline), and twenty-five mice were induced intraperitoneally with 10 mg/kg BW AOM (Azoxymethane), and it was followed by the administration of 5% DSS (Dextran Sodium Sulfate) every two weeks for 20 weeks. At the sixth week, one mice in each group was sacrificed for the Fecal Occult Bold Test (FOBT) and serum amyloid α (SAA) test to ensure that CAC was indeed formed. The administration of EPE with varying doses was started from the eighth week and was continued until the 21^st week. The length of the colonic crypt was measured through histology appearance using Hematoxylin-eosin (HE) staining while the immunohistochemical method was used to observe apoptotic activity through the enhancement of caspase-3 expression. The results showed that the increase in EPE dosage also increased the crypt colon length compared to the positive control group. The administration of 1.00 mg/20gBW EPE significantly increased cell apoptosis which can be observed through caspase-3 expression compared to the positive control group (p<0.05). Based on these data, the extract of EPE can be developed as phytotherapy for chemopreventive.

Author Biographies

Riza Ambar Sari, Department of Pharmacy, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144

Rahmi Annisa, Department of Pharmacy, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144

Risma Aprinda Kristanti, Department of Biomedic, Maulana Malik Ibrahim State Islamic University, Malang, Jl. Gajayana No. 50 Malang Indonesia 65144

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