Pengendalian Hayati Penyakit Layu Bakteri Tembakau: 2. Percobaan di Rumah Kaca

https://doi.org/10.22146/jpti.9967

Triwidodo Arwiyanto(1*), I. Hartana(2)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Asosiasi Penelitian Perkebunan Indonesia
(*) Corresponding Author

Abstract


Tobacco bacterial wilt caused by Ralstonia solanacearum has been controlled biologically in the greenhouse by the use of rhizosphere microorganisms, i.e. fluorescent pseudomonads and Bacillus spp. Root dipping for 30 minutes in the antagonist suspension (108 cfu/ml) suppressed bacterial wilt development. The best results were obtained with fluorescent pseudomonads Pf-20 and Bacillus sp. ba-118 isolates but the repression was annulled with the use of its combination. Disease severity were higher when the plants were treated with the combination between fluorescent pseudomonads. Treatment combinations between Pf-28, Pf-31, Pf-33, and Ba-118 could reduce the disease index.


Keywords


tobacco; bacterial wilt; biological control

Full Text:

PDF


References

Arshad, M. & W.T. Frankenberger Jr. 1993. Microbial Production of Plant Growth Regulators. pp. 307-348. In LB. Metting Jr. (ed.) Soil Microbial Ecology. Application in Agricultural and Environmental Management. Marcel Dekker, Inc. New York.

Arwiyanto, T.; M. Goto; S. Tsuyunu & Y. Takikawa. 1994. Biological Control of Tomato Bacterial Wilt with the use of Avirulent Strain of Pseudomonas solanacearum Isolated from Strelitzia reginae. Ann. Phytopath. Soc. Japan 60:421-430

Arwiyanto, T. 1995. Strategi Pengendalian Penyakit Layu Bakteri Tembakau Cerutu di Sumatera Utara Secara Terpadu. Ekspose Hasil Penel. Tembakau 1995, Medan. 34p.

Arwiyanto, T. 1997. Pengendalian Hayati Penyakit Layu Bakteri Tembakau. 1. Lsolasi Bakteri Antagonis. J. Perlind. Tan. Indon. 3: 44-60

Cook, RJ. & Baker, K.F. 1983. The Nature and Practice of Biological Control of Plant Pathogens. APS Press, St. Paul, Minnesota. 539 p.

Campbell, R. 1989. Biological Control of Microbial Plant Pathogens. Cambridge Univ. Press, Cambridge.

Defago, G; C.H. Berling; U. Burger; D. Haas; G. Kahr; C. Keel; C. Voistard; Wirthner; & B. Wurthnich. 1990. Suppress ion of Black Root of Tobacco and other Root Diseases by Strains of Pseudomonas fluorescens: Potential Applications and Mechanisms. p. 75-81. In: D. Homby (ed.) Biological Control of Soilborne Plant Pathogens, CAB Intemat. Wallingford.

Diany, V. & Arwiyanto, T. 1996. Pengendalian Hayati Penyakit Layu Bakteri Ternbakau dengan pseudomonad fluoresen. 1. Isolasi Bakteri Antagonis. Sem. Reg. III PFI Komda Jawa Tengah dan DIY, Salatiga.

Fravel, D.R. 1988. Role of Antibiosis in the Biocontrol of Plant Diseases. Ann. Rev. Phytopathol. 26: 75-91

Fravel, DR. & RIP. Larkin. 1996. Availability and Application of Biocontrol Products. BioL Cult. I Tests for Control of Plant Dis. 11: 1-7

Goto, M. 1992. Fundamentals of Bacterial Plant Pathology. Aced. Press. San Diego. 342p.

Hartana, 1. 1978. Budidaya Tembakau Cerutu I. Masa Pra-Panen. Balai Penelit. Perkeb. Jember. 107 p.

Kelman, A. 1953. The bacterial wilt caused by Pseudomonas solanacearwn. North Carolina Agric. Exp. Sta. Tech. Bull. 99.

Kloepper, J.W. 1993. Plant Growth - Promoting Rhizobacteria as Biological Control Agents. pp. 255-274. In : J.B. Metting Jr. (ed.) Soil Microbial Ecology Application in Agricultural and Environmental Management. Marcel Dekker, Inc. New York.

Kloepper, J.W., M.N. Schroth & T.D. Miller. l90. Effect of Rhizosphere Colonization by Plant Growth-Promoting Rhizobacteria on Potato Plant development and Yield. Phytopathology 70: 1078-1082.

Risamena, J. & Arwiyanto, T. 1996. Pengendalian Hayati Penyakit Layu Bakteri Tembakau dengan Bacillus spp.. 1. Isolasi Bakteri Antagonis. Sem. Reg. PFI Komda Jawa Tengah dan DIY, Salatiga.

Stolp, H & Gadkani. 1991. Nonpathogenic Members of the Genus Pseudomonas. p.719- 741. In: M.P. Starr, H. Stoip, H.G. Truper, A. Balows, and H.G. Schlegel (eds.) The Prokaryotes; A handbook on Habitats, 1solation. and Identification of Bacteria. Vol 1. Springer-Verlag. Berlin.

Suslow, T.V. 1982. Role of Root-Colonizing Bacteria in Plant Growth. In: MS. Mount and Gil. Lacy (eds.), Phytopathogenic Prokaryotes. p. 187-223. Vol 1. Academic Pres & New York.

Weller, D.M. 1985. Application of Fluorescent Pseudomonads to Control Root Diseases. pp. 137-140. In: C.A. Parker, A.D. Rovira., KJ. Moore, and P.T.W. Wong (eds.), Ecology and Management of Soilborne Plant Pathogens. Proc. Fourth Internat. Congr. of Plant Pathol. Section 5. Amer. Phytopath. Soc. St Paul, Minnesota.

Weller, D.M. 1988. Biological Control of Soilborne Plant Pathogens in the Rhizosphere with Bacteria. Ann. Rev. Phytopathol. 28: 379-407



DOI: https://doi.org/10.22146/jpti.9967

Article Metrics

Abstract views : 4891 | views : 1934

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 1999 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics