Pengendalian Hayati Penyakit Layu Fusarium Pisang (Fusarium oxysporum f.sp. cubense) dengan Trichoderma sp.

https://doi.org/10.22146/jpti.9396

Albertus Sudirman(1*), Christanti Sumardiyono(2), Siti Muslimah Widyastuti(3)

(1) Politeknik Negeri Lampung
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Kehutanan Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The aim of this research was to study the inhibiting ability of Trichoderma sp. to control fusarium wilt of banana in greenhouse condition. The experiments consisted of the antagonism test between Trichoderma sp. and Fusarium oxysporum f.sp. cubense (Foc) in vitro using dual culture method and glass house experiment which was arranged in 3×3 Factorial Complete Randomized Design. First factor of the latter experiment was the dose of Trichoderma sp. culture (0, 25, and 50 g per polybag), second factor was time of Trichoderma culture application (2 weeks before Foc inoculation, at same time with Foc inoculation and 2 weeks after Foc inoculation). Trichoderma sp. was cultured in mixed rice brand and chaff medium. The disease intensity was observed with scoring system of wilting leaves (0–4). The results showed that Trichoderma sp. was antagonistic against Foc in vitro and inhibited 86% of Foc colony development. Mechanism of antagonism between Trichoderma sp. and Foc was hyperparasitism. Trichoderma hyphae coiled around Foc hyphae. Lysis of Foc hyphae was occurred at the attached site of Trichoderma hyphae on Foc hyphae. Added banana seedling with Trichoderma sp. Culture reduced disease intensity of Fusarium wilt. Suggested dose of Trichoderma culture application in glass house was 25 g/polybag, given at the same time with Foc inoculation.


Keywords


biological control; Trichoderma sp.; Fusarium wilt of banana; Foc

Full Text:

PDF


References

Blakeman, J.P. & N.J. Fokhema. 1982. Potential for Biological Control of Plant Disease on the Phylloplane. Annual Review of Phytopathology 20: 167–192.

Booth, C. 1971. The Genus Fusarium. Commonwealth Mycological Institute. Kew Surrey, England, 237 p. Brown, J. & H. Ogle 1997. Fungal Diseases and their Control, p. 443–466. In J. Brown & H. Ogle (eds), Plant Pathogens and Plant Diseases. The University of New England Printery, Armidal.

Chet, I. 1990. Biological Control of Soil-borne Plant Pathogen with Fungal Antagonists in Combination with Soil Treatment, p. 15–25. In D. Hornby (ed.), Biological Control of Soil-borne Plant Pathogens. CAB International, Wallingford, United Kingdom.

Gao, H. C.H. Beckman, & W.C. Muller. 1994. The Rate of Vascular Colonization as a Measure of the Genotype Interaction between Various Cultivars of Tomato and Various Formae or Races of Fusarium oxysporum. Physiological and Molecular Plant Pathology 46: 29–43.

Hadar, Y., I. Chet, & Y. Henis. 1979. Biological Control of Rhizoctonia solani Damping off with Wheat Brand Culture of Trichoderma harzianum. Phytopathology 69: 64–68.

Harjono. 2001. Antifungal Activity of Purified Endochitinase Produced by Biocontrol Agent Trichoderma reesei against Ganoderma philipii. Pakistan Journal of Biological Sciences 4: 1232–1234.

Harman, G.E., I. Chet, & R. Baker. 1981. Factors Affecting Trichoderma hamatum Applied to Seed as a Biocontrol Agent. Phytopathology 71: 569–572.
Semangun, H. 2000. Penyakit-Penyakit Tanaman Hortikultura. Gadjah Mada University Press, Yogyakarta. 850 p.

Sivan, A., U. Ona & I. Chet 1986. Biological Control of Fusarium spp. in Cotton, Wheat and Muskmelon by Trichoderma harzianum. Phytopathology 116: 39–47.

Sulistianingsih, N., Djajati, S. Santoso, & L. Sulistyowati. 1995. Pengaruh Inokulasi Jamur Trichoderma sp. terhadap Penyakit Busuk Batang Vanili oleh F. batatatis var. vanillae (Tucker), p. 374–376. In Parman (ed.), Prosiding Kongres Nasional XIII dan Seminar Ilmiah Perhimpunan Fitopatologi Indonesia. Mataram, 27–29 September 1995.

Thurston, H.D. 1984. Tropical Plant Diseases. APS Press. St. Paul, Minnesota. 208 p.

Widyastuti, S.M. & Sumardi. 1998. Antagonistic Potential of Trichoderma spp. against Root Rot Pathogen of Forest Tree Species. Asian Journal of Sustainable Agriculture 1: 1–8.

Widyastuti, S.M., Sumardi, & N. Hidayati. 1998a. Kemampuan Trichoderma spp. untuk Pengendalian Hayati Jamur Akar Putih pada Acacia mangium secara In vitro. Buletin Kehutanan 36: 24–38.

Widyastuti, S.M., Sumardi, A. Sulthoni, & Harjono. 1998b. Pengendalian Hayati Penyakit Akar Merah pada Akasia dengan Trichoderma spp. Jurnal Perlindungan Tanaman Indonesia 4: 65–72.



DOI: https://doi.org/10.22146/jpti.9396

Article Metrics

Abstract views : 7465 | views : 13947

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2011 Jurnal Perlindungan Tanaman Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.  

View website statistics