Elektron Mikroskopi dan Imunogenisitas Baculovirus oryctes Isolat Yogyakarta
Y. B. Sumardiyono(1*)
(1) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Palm rhinoceros beetle (Oryctes rhinoceros) was infected per os with Yogyakarta isolate of Baculovirus oryctes in laboratory condition. Midguts of infected beetle obtained were then extracted for further nucleoprotein purification by centrifugation method. Electron microscopy studies on purified nucleoprotein revealed rod-shape viruses with rounded end measured 190×94 nm in average. One end of the particle showed tail-like structure. Antibodies against the virus were obtained by immunization to rabbit, and reacted against either purified virus or extract of infected beetle, but not against extract of healthy beetle.
Keywords
Full Text:
PDFReferences
Bedford. G.O. 1981. Control, of the rhinoceros beetle by Baculovirus. In : Microbial Control of Pests and Plant Disease 1970 -1980 ( Ed. : Burges, H.D.) Acad. Press. London : 406 - 427.
Huger, A.M. 1966. A virus disease of the Indian Rhinoceros Beetle, Oryctes rhynoceros L. caused a new type of insect virus. J. Invert. Pathol. 8: 38
Huger, A.M. and A. Krieg. 1991. Baculoviridae. Nonoccluded Baculoviruses. In : Atlas of invertebrate viruses (Eds.; Adam J.R. and J. Bonaini) CRS Press: 187- 319.
Kobayashi, J. and S. Somowiyarjo. 1994. Properties of Oryctes Baculovirus isolated in Indonesia. Manuscript for publication.
Koenig, R. 1981. Indirect ELISA methods for the broad specificity detection of plant viruses. J. Gen. Virol. 40: 309 - 314.
Monsarrat, P. J.C. Veyrunes, G. Meynadier, G. Croizicr, C. Vago. 1973. Purification et etude structurale du virus du coleoptere Oryctes rhinoceros. C.R Acvad. Sc Paris 277: 1413 1415.
Payne, C.C. 1974. The isolation and characterization of a virus from Oryctes rhinoceros. J. Gen. Virol. 25: 105 - 116.
Zelazny, B. 1976. Transmission of a baculovirus in population of Oiyctes rhinoceros. 1. Invert. Pathol. 27: 221 -227.
DOI: https://doi.org/10.22146/jpti.9349
Article Metrics
Abstract views : 978 | views : 1006Refbacks
- There are currently no refbacks.
Copyright (c) 1995 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics