Utilization of Arbuscular Mycorrhizal Fungi and Bacillus velezensis Inoculation in Suppressing Twisted Disease of Shallot

https://doi.org/10.22146/jpti.89296

Jilan Tsani Abdullah(1), Suryanti Suryanti(2), Tri Joko(3*)

(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(*) Corresponding Author

Abstract


Twisted disease is one of the problems in shallot cultivation. The application of the biological agents Bacillus velezensis and Arbuscular Mycorrhizal Fungi is an alternative expected to overcome disease problems in shallot plantings. The purpose of this study was to determine the effect of the application of B. velezensis and Arbuscular Mycorrhizal Fungi on the growth and health of shallot. The research was conducted in Gotakan, Panjatan, Kulon Progo, and the Phytopathology Laboratory, Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta. Shallot treatments with the application of biological agents were carried out either individually by soaking the bulbs in B. velezensis suspension before planting, coating the bulbs using Arbuscular Mycorrhizal Fungus (Rhizophagus intraradices) with a carrier medium of kaolin flour before planting, and a combination of spraying the B. velezensis suspension on plants at two-week intervals during the growth period and coating the bulbs before planting with R. intraradices. The results of the study showed that the shallot-applied single application of B. velezensis reduced the intensity and incidence of twisted disease by 2.51% and 37.6%, respectively. The combination treatment of B. velezensis and R. intraradices was able to increase the resistance of bulbs to postharvest pathogen Fusarium solani infection, with infected bulbs and areas of 0.70% and 0.71%, respectively.


Keywords


Arbuscular Mycorrhizal Fungi (AMF); Bacillus velezensis; damage; Fusarium sp.; shallots

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References

Al-Askar, A.A., & Rashad, Y. (2010). Arbuscular Mycorrhizal Fungi: A Biocontrol Agent against Common Bean Fusarium Root Rot Disease. Plant Pathology Journal 9(1): 31-38. 10.3923/ppj.2010.31.38

Artanti, H., Joko, T., Somowiyarjo, S., & Suryanti. (2022). The Potential of Rhizophagus intraradices and Trichoderma asperellum to Induce Shallot Resistance against Twisted Disease. Jurnal Perlindungan Tanaman Indonesia, 26(1), 57-66. https://doi.org/10.22146/jpti.70673

Chen, L., Heng, J., Qin, S., & Bian, K. (2018). A Comprehensive Understanding of The Biocontrol Potential of Bacillus velezensis LM2303 Against Fusarium Head Blight. PLoS One, 13(6), e0198560. https://doi.org/10.1371/journal.pone.0198560

Damanik, S.A., & Suryanto, A. (2018). Efektivitas Penggunaan Mikoriza dan PGPR (Plant Growth Promoting Rhizobacteria) Terhadap Tanaman Bawang Merah (Allium ascalonicum L.) pada Pipa PVC Sistem Vertikultur. Jurnal Produksi Tanaman, 6(4), 635-641.

Fitriani, M.L., Wiyono, S., & Sinaga, M.S. (2019). Potensi Kolonisasi Mikoriza Arbuskular Dan Cendawan Endofit dan Kemampuannya dalam Pengendalian Layu Fusarium pada Bawang Merah. Jurnal Fitopatologi Indonesia, 15(6), 228-238. https://doi.org/10.14692/jfi.15.6.228-238

Ilmiah, H.H., Sulistyaningsih, E., & Joko, T. (2021). Fruit Morphology, Antioxidant Activity, Total Phenolic and Flavonoid Contents of Salacca zalacca (Gaertner) Voss by Applications of Goat Manures and Bacillus velezensis B-27. Caraka Tani: Journal of Sustainable Agriculture, 36(2), 270-282, https://doi.org/10.20961/carakatani.v36i2.43798

Ismiyatuningsih, Joko, T., & Hartono, S. (2016). Survey and Detection of Pectobacterium atrosepticum in Major Potato-Growing Areas in Central Java Province, Indonesia. Ilmu Pertanian 1(1), 1−6. https://doi.org/10.22146/ipas.11654

Jeffries, P., Gianinazzi, S., Perotto, S., Turnau, K., & Barea, J.M. (2003). The Contribution of Arbuscular Mycorrhizal Fungi in Sustainable Maintenance of Plant Health and Soil Fertility. Biology and Fertility of Soils, 37, 1-16. https://doi.org/10.1007/s00374-002-0546-5

Jiang, C.H., Yao, X.F., Mi, D.D., Li, Z.J., Yang, B.Y., Zheng, Y., Qi, Y.J., & Guo, J.H. (2019). Comparative Transcriptome Analysis Reveals the Biocontrol Mechanism of Bacillus velezensis F21 against Fusarium Wilt on Watermelon. Frontiers in Microbiology, 10, 652. https://doi.org/10.3389/fmicb.2019.00652

Köycü, N.D., & Oezer, N. (1997). Determination of Seedborne Fungi in Onion and Their Transmission to Onion Sets. Phytoparasitica, 25(1), 25-31. https://doi.org/10.1007/BF02981476

Moradi-Pour, M., R. Saberi-Riseh, K. Esmaeilzadeh-Salestani, R. Mohammadinejad, & E. Loit. (2021). Evaluation of Bacillus velezensis for Biological Control of Rhizoctonia solani in Bean by Alginate/Gelatin Encapsulation Supplemented with Nanoparticles. Journal of Microbiology and Biotechnology, 31(10), 1373–1382 https://doi.org/10.4014/jmb.2105.05001

Nadeem, S.M., Ahmad, M., Zahir, Z.A., Javaid, A., & Ashraf, M. (2014). The Role of Mycorrhizae and Plant Growth Promoting Rhizobacteria (PGPR) in Improving Crop Productivity Under Stressful Environments. Biotechnology Advances, 32(2), 429-448, https://doi.org/10.1016/j.biotechadv.2013.12.005

Nugroho, A.W., Hadiwiyono, & Sudadi. (2015). Potensi Jamur Perakaran sebagai Agens Pengendalian Hayati Penyakit Moler (Fusarium oxysporum f.sp. cepae) pada Bawang Merah. Agrosains: Jurnal Penelitian Agronomi, 17(1), 4-8. https://doi.org/10.20961/agsjpa.v17i1.18656

Palencia, P., Martínez, F., Pestana, M., Oliveira, J. A., & Correia, P. J. (2015). Effect of Bacillus velezensis and Glomus intraradices on fruit quality and growth parameters in strawberry soilless growing system. The Horticulture Journal, 84(2), 122-130. https://doi.org/10.2503/hortj.MI-002

Prakoso, E. B., Wiyatinigsih, S., & Nirwanto, H. (2017). Uji Ketahanan Berbagai Kultivar Bawang Merah (Allium ascalonicum) terhadap Infeksi Penyakit Moler (Fusarium oxysporum f.sp. cepae). Berkala Ilmiah Agroteknologi-PLUMULA, 5(1), 10-20. Retrieved from http://www.ejournal.upnjatim.ac.id/index.php/plumula/article/view/773

Rahma, A.A., Suryanti, Somowiyarjo, S., & Joko, T. (2020). Induced Disease Resistance and Promotion of Shallot Growth by Bacillus velezensis B-27. Pakistan Journal of Biological Sciences, 23(9), 1113-1121. https://doi.org/10.3923/pjbs.2020.1113.1121

Sevirasari, N., Sulistyaningsih, E., Kurniasih, B., Suryanti, Wibowo, A., & Joko, T. (2022). Effects of Relay Intercropping Model and Application of Biological Agents on The Growth and Yield of Hot Pepper. Ilmu Pertanian (Agricultural Sciences), 7(1), 35-46. https://doi.org/10.22146/ipas.69078

Wang, C., Zhao, D., Qi, G., Mao, Z., Hu, X., Du, B., Liu, K. & Ding, Y. (2020). Effects of Bacillus velezensis FKM10 for Promoting the Growth of Malus hupehensis Rehd. and Inhibiting Fusarium verticillioides. Frontiers in Microbiology, 10, 2889. https://doi.org/10.3389/fmicb.2019.02889

Wibawa, F.S., Rokhminarsi, E., & Leana, N.W.A. (2023). Pengaruh Pemberian Campuran Mikoriza-Trichoderma sp. dan Pengurangan Dosis Pupuk NPK terhadap Penyimpanan Umbi Bawang Merah. Jurnal AGRO, 10(1), 149-163. https://doi.org/10.15575/24245

Wibowo, E.P., Widiastuti, A., Joko, T., Suryanti, & Priyatmojo, A. (2022). Effect of Biocontrol Agent (Bacillus and Mycorrhizal Fungi) Application against Twisted Disease (Fusarium spp.) in Off-Season Shallot Production. Jurnal Perlindungan Tanaman Indonesia, 26(2), 1-18. https://doi.org/10.22146/jpti.75579

Widyaningsih, S., Utami, S.N.H., Joko, T., & Subandiyah, S. (2017). Development of Disease and Growth on Six Scion/Rootstock Combinations of Citrus Seedlings under Huanglongbing Pressure. Journal of Agricultural Science, 9(6), 229-238. https://doi.org/10.5539/jas.v9n6p229

Widyaningsih, S., Utami, S.N.H., Joko, T., & Subandiyah, S. (2019). Plant Response and Huanglongbing Disease Development against Heat Treatments on ‘Siam Purworejo’ (Citrus nobilis (Lour)) and ‘Nambangan’ (C. maxima (Burm.) Merr.) under Field Condition. Archives of Phytopathology and Plant Protection, 52(3), 259-276. https://doi.org/10.1080/03235408.2018.1544193

Wisanggeni, G.A., Suryanti, & Joko, T. (2023). The Potential of Bacillus subtilis subsp. subtilis RJ09 as a Biological Control Agent against Leaf Spot Diseases on Clove. Jurnal Fitopatologi Indonesia, 19(3), 118-126. https://doi.org/10.14692/jfi.19.3.118-126

Wulan, E. I. R., Wibowo, A., Joko, T., & Widiastuti, A. (2022). Induced Resistance Mechanism of Twisted Disease Suppression of Shallot by Bacillus spp. Jurnal Perlindungan Tanaman Indonesia, 26(1), 40-50. https://doi.org/10.22146/jpti.73198

Ye, M., Tang, X., Yang, R., Zhang, H., Li, F., Tao, F., Li, F., & Wang, Z. (2018). Characteristics and Application of a Novel Species of Bacillus: Bacillus velezensis. ACS Chemical Biology, 13(3), 500-505. https://doi.org/10.1021/acschembio.7b00874

Zaid, D. S., Cai, S., Hu, C., Li, Z., & Li, Y. (2022). Comparative Genome Analysis Reveals Phylogenetic Identity of Bacillus velezensis HNA3 and Genomic Insights into Its Plant Growth Promotion and Biocontrol Effects. Microbiology Spectrum, 10(1), e02169-21. https://doi.org/10.1128/spectrum.02169-21



DOI: https://doi.org/10.22146/jpti.89296

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