In Vitro Effectiveness of Beauveria bassiana as a Control Agent against Invasive Fall Armyworm (Spodoptera frugiperda) Larvae

https://doi.org/10.22146/jpti.74940

Eny Wahyuning Purwanti(1*), Julisa Ginti Mulyasari(2), Budianto Budianto(3)

(1) Department of Agriculture, Polytechnic Agricultural Development Malang Jln. Dr. Cipto No. 144a, Bedali, Lawang, Malang, East Java, 65200 Indonesia
(2) Department of Agriculture, Polytechnic Agricultural Development Malang Jln. Dr. Cipto No. 144a, Bedali, Lawang, Malang, East Java, 65200 Indonesia
(3) Department of Agriculture, Polytechnic Agricultural Development Malang Jln. Dr. Cipto No. 144a, Bedali, Lawang, Malang, East Java, 65200 Indonesia
(*) Corresponding Author

Abstract


Fall armyworm or Spodoptera frugiperda is one of the main pests of corn. It can caused up to 28.08% yield loss. Pest control efforts with broad-spectrum insecticides can cause negative impacts that disrupt ecosystems, non-target biotic factors and the surrounding environment. Beauveria bassiana is an alternative biological control agent that is target-specific. This study was conducted to determine toxic and antifeedant activities of B. bassiana application on third to fifth-instar S. frugiperda larvae. B. bassiana isolates were propagated using corn flakes media and resulted in colonies with conidia density of 1.1×108conidia.g-1. This study used a completely randomized design with 5 treatments and 5 replications. Treatments consisted of P1 namely B. bassiana propagation with a concentration of 60 g.L-1, P2 = 70 g.L-1, and P3 = 80 g.L-1. P0 was a negative control (untreated with B. bassiana) and P4 was a positive control (diazinon with concentrations of 2 ml.L-1). The variables observed were mortality and weight of the leftover feed. The results showed that the formulation of B. bassiana with concentrations of 80 g.L-1 was able to infect armyworms with a mortality percentage of 53.5% which was statistically similar as the effect of chemical insecticides. In addition, inhibition of feeding activity due to treatment could prevent leaf damage up to 68.21%.


Keywords


antifeedants; biological agents; entomopathogenic fungi; larvae; pests

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References

Fateha, R. N., Grasela, M., Ichwan, M. N., Purwanti, E. W., & Kurniasari, I. (2020). Larvicidal and Antifeedant Activities of Clove Leaf Oil against Spodoptera litura (F.) on Soybean. Jurnal Hama dan Penyakit Tumbuhan Tropika, 21(1), 20–25. https://doi.org/10.23960/j.hptt.12120-25

González-Mas, N., Ortega-García, L., Garrido-Jurado, I., Dembilio, O., Jaques, J. A., & Quesada-Moraga, E. (2019). Which Came First: The Disease or the Pest? Is There a Host Mediated Spread of Beauveria bassiana (Ascomycota: Hypocreales) by Invasive Palm Pests? Journal of Invertebrate Pathology, 162, 26–42. https://doi.org/10.1016/j.jip.2019.01.007

Grijalba, E. P., Espinel, C., Cuartas, P. E., Chaparro, M. L., & Villamizar, L. F. (2018). Metarhizium rileyi Biopesticide to Control Spodoptera frugiperda: Stability and Insecticidal Activity under Glasshouse Conditions. Fungal Biology, 122(11), 1069–1076. https://doi.org/10.1016/j.funbio.2018.08.010

Herlinda, S., Suharjo, R., Elbi Sinaga, M., Fawwazi, F., & Suwandi, S. (2021). First Report of Occurrence of Corn and Rice Strains of Fall Armyworm, Spodoptera frugiperda in South Sumatra, Indonesia and its Damage in Maize. Journal of the Saudi Society of Agricultural Sciences, 20(7), 1–8. https://doi.org/10.1016/j.jssas.2021.11.003

Herlinda, S., Utama, M. D., & Pujiastuti, Y. (2006). Kerapatan dan Viabilitas Spora Beauveria bassiana (Bals.) akibat Subkultur dan Pengayaan Media serta Virulensinya terhadap Larva Plutella xylostella (Linn.) [Density and Viability of Spores of Beauveria bassiana (Bals.) Vuill. due to Sub-cultures and Media Enriched, and Its
Virulence against Larvae of Plutella xylostella (Linn.)]. Jurnal Hama dan Penyakit Tumbuhan Tropika, 6(2), 70–78. https://doi.org/https://doi.org/10.23960/j.hptt.2670-78

Indriyanti, D. R., Wijayanti, D., & Setiati, N. (2021). The Effect of Beauveria bassiana on the Larvae of Oryctes rhinoceros. Journal of Physics: Conference Series, 1918(5). https://doi.org/10.1088/1742-6596/1918/5/052091

Indriyanti, D. R., Mahmuda, S., & Slamet, M. (2017). Effect of Beauveria bassiana Doses on Spodoptera litura Mortality. International Journal of Scientific & Technology Research, 6(9), 206–210.

Ishak, S. Y., Bahua, M. I., & Limonu, M. (2013). Pengaruh Pupuk Organik Kotoran Ayam terhadap Pertumbuhan Tanaman Jagung (Zea mays L.) di Dulomo Utara Kota Gorontalo. Journal of Applied Testing Technology, 2(1), 210–218.

Kasryno, Faisal, Pasandaran, E., & Fagi, A. M. (2015). Dinamika Produksi dan Pembangunan Sistem Komoditi Jagung di Indonesia. In F. Kasryno, E. Pasandaran, & A. M. Fagi (Eds.), Ekonomi Jagung Indonesia (2nd ed., pp. 30–35). Badan Penelitian dan Pengembangan Pertanian Departemen Pertanian.

Lubis, A. A. N., Anwar, R., Soekarno, B. P., Istiaji, B., Sartiami, D., Irmansyah, & Herawati, D. (2020). Serangan Ulat Grayak Jagung (Spodoptera frugiperda) pada Tanaman Jagung di Desa Petir, Kecamatan Daramaga, Kabupatem Bogor dan Potensi Pengendaliannya Menggunakan Metarizhium rileyi. Jurnal Pusat Inovasi Masyarakat, 2(6), 931–939.

Mascarin, G. M., Jackson, M. A., Kobori, N. N., Behle, R. W., & Delalibera Júnior, Í. (2015). Liquid Culture Fermentation for Rapid Production of Desiccation Tolerant Blastospores of Beauveria bassiana and Isaria fumosorosea Strains. Journal of Invertebrate Pathology, 127, 11–20. https://doi.org/10.1016/j.jip.2014.12.001

Meyling, N. V., & Eilenberg, J. (2007). Ecology of the Entomopathogenic Fungi Beauveria bassiana and Metarhizium anisopliae in Temperate Agroecosystems: Potential for Conservation Biological Control. Biological Control, 43(2), 145–155. https://doi.org/10.1016/j.biocontrol.2007.07.007

Muhadjir, F. (2018). Karakteristik Tanaman Jagung. Retrieved from http://balitsereal.litbang.pertanian.go.id/wp-content/uploads/2018/08/3karakter.pdf

Navik, O., Shylesha, A. N., Patil, J., Venkatesan, T., Lalitha, Y., & Ashika, T. R. (2021). Damage, Distribution and Natural Enemies of Invasive Fall Armyworm Spodoptera frugiperda (J. E. Smith) under Rainfed Maize in Karnataka, India. Crop Protection, 143, 105536. https://doi.org/10.1016/j.cropro.2021.105536

Nurmaisah, & Purwati, N. (2021). Identifikasi Jenis Serangga Hama pada Tanaman Jagung (Zea mays) di Kota Tarakan. Jurnal Proteksi Tanaman Tropis, 2(1), 19–22. https://doi.org/10.19184/jptt.v2i1.21607

Sopialena. (2018). Pengendalian Hayati dengan Memberdayakan Potensi Mikroba. Samarinda, Indonesia: Mulawarman University Press.

Subiono, T. (2019). Preferensi Spodoptera frugiperda (Lepidoptera: Noctuidae) pada Beberapa Sumber Pakan [Preferences of Spodoptera frugiperda (Lepidoptera: Noctuidae) in Several Feed Sources]. Jurnal Agroekoteknologi Tropika Lembab, 2(2), 130–134. https://doi.org/10.35941/jatl.2.2.2020.2813.130-134

Suharto. (2004). Patogenisitas Beberapa Isolat Beauveria bassiana pada Plutella xylostella [Pathogenicity of Beauveria bassiana Isolates on Plutella xylostella]. Jurnal Perlindungan Tanaman Indonesia, 10(1), 8–12. Retrieved from https://jurnal.ugm.ac.id/jpti/article/view/12207

Suharto, Trisusilowati, E. B., & Purnomo, H. (1998). Kajian Aspek Fisiologik Beauveria bassiana dan Virulensinya terhadap Helicoverpa armigera [Study on Physiological Aspects of Beauveria bassiana and Their Virulence to Helicoverpa armigera]. Jurnal Perlindungan Tanaman Indonesia, 4(2), 112–119. Retrieved from https://jurnal.ugm.ac.id/jpti/article/view/9919

Sunari, A. A. A. A. S., Sumiartha, I. K., Supartha, I. W., Mahaputra, I. G. F., Yudha, I. K. W., Utama, I. W. E. K., & Wiradana, P. A. (2022). Potential Yield Reduction of Sweet and Glutinous Corn Varieties Damaged by the Invasive Pest Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae): Field Trial Scale. Proceedings of the 2nd International Conference on Education and Technology (ICETECH 2021): Advances in Social Science, Education and Humanities Research, 630, 14–19. https://doi.org/10.2991/assehr.k.220103.003



DOI: https://doi.org/10.22146/jpti.74940

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