In Vitro Effectiveness of Beauveria bassiana as a Control Agent against Invasive Fall Armyworm (Spodoptera frugiperda) Larvae

https://doi.org/10.22146/jpti.74940

Eny Wahyuning Purwanti(1*), Julisa Ginti Mulyasari(2), Budianto Budianto(3)

(1) Department of Agriculture, Polytechnic Agricultural Development Malang Jln. Dr. Cipto No. 144a, Bedali, Lawang, Malang, East Java, 65200 Indonesia
(2) Department of Agriculture, Polytechnic Agricultural Development Malang Jln. Dr. Cipto No. 144a, Bedali, Lawang, Malang, East Java, 65200 Indonesia
(3) Department of Agriculture, Polytechnic Agricultural Development Malang Jln. Dr. Cipto No. 144a, Bedali, Lawang, Malang, East Java, 65200 Indonesia
(*) Corresponding Author

Abstract


Fall armyworm or Spodoptera frugiperda is one of the main pests of corn. It can caused up to 28.08% yield loss. Pest control efforts with broad-spectrum insecticides can cause negative impacts that disrupt ecosystems, non-target biotic factors and the surrounding environment. Beauveria bassiana is an alternative biological control agent that is target-specific. This study was conducted to determine toxic and antifeedant activities of B. bassiana application on third to fifth-instar S. frugiperda larvae. B. bassiana isolates were propagated using corn flakes media and resulted in colonies with conidia density of 1.1×108conidia.g-1. This study used a completely randomized design with 5 treatments and 5 replications. Treatments consisted of P1 namely B. bassiana propagation with a concentration of 60 g.L-1, P2 = 70 g.L-1, and P3 = 80 g.L-1. P0 was a negative control (untreated with B. bassiana) and P4 was a positive control (diazinon with concentrations of 2 ml.L-1). The variables observed were mortality and weight of the leftover feed. The results showed that the formulation of B. bassiana with concentrations of 80 g.L-1 was able to infect armyworms with a mortality percentage of 53.5% which was statistically similar as the effect of chemical insecticides. In addition, inhibition of feeding activity due to treatment could prevent leaf damage up to 68.21%.


Keywords


antifeedants; biological agents; entomopathogenic fungi; larvae; pests

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DOI: https://doi.org/10.22146/jpti.74940

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