Biological Effects of Indigenous Entomopathogenic Fungi and Their Application Methods on Spodoptera frugiperda

Endang Warih Minarni(1*), Nurtiati Nurtiati(2), Dina Istiqomah(3)

(1) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. Dr. Soeparno No 61, Purwokerto, Central Java 53123 Indonesia
(2) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. Dr. Soeparno No 61, Purwokerto, Central Java 53123 Indonesia
(3) Department of Agrotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman Jln. Dr. Soeparno No 61, Purwokerto, Central Java 53123 Indonesia
(*) Corresponding Author


Spodoptera frugiperda is a new pest in Indonesia that attacks corn and can cause up to 100 percent damageon young plants. As an invasive pest, information on potential indigenous natural enemies that cancontrol this pest is needed. This study aims to determine the effectiveness of indigenous entomopathogenic fungi and their application methods on mortality, feeding activity, growth, fecundity, and fertility of S. frugiperda. This study used a factorial Completely Randomized Block Design (RCBD) method consisting of 10 treatments. Treatmentstested werecombination of fungi species, namely Fusarium sp., Aspergillus oryzae, Entomophthora sp. with conidia density 109 ml-1, distilled water and cypermethrin at concentration of 1 ml.L-1as a control. Two application techniques used were direct application on S. frugiperda larvae and diet test. Each treatment was repeated three times resulting in30 experimental units. Variables observed included mortality, feeding activity, growth, fecundity, and fertility of S. frugiperda. Results showed that the three fungi and cypermethrin treatment did not cause mortality, but reduced feeding activity, fecundity and fertility of S. frugiperda. Application of entomopathogenic fungi ondietswas more effective than directly spraying S. frugiperda larvae. The best treatment combination that suppressed feeding activity was the application of Aspergillus oryzae sprayed on S. frugiperda diet.It was also suspected that S. frugiperda larvae used in this test had developed resistance to cypermethrin.


Aspergillus oryzae; Entomophthora sp.; Fusarium sp.; indigenous; Spodoptera frugiperda

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Afriyanita, Lina, E. C., & Darnetty. (2019). Aktivitas Insektisida Ekstrak Air Campuran Buah Piper aduncum dan Daun Tephrosia vogelii terhadap Crocidolomia pavonana Fabricius (Lepidoptera: Crambidae) [Water Extract Activities of Piper aduncum inflorescences and Tephrosia vogelii Leaves as Insecticide to Crocidolomia pavonana Fabricius (Lepidoptera: Crambidae)]. JPT: Jurnal Proteksi Tanaman (Journal of Plant Protection), 3(1), 34‒46.

Babu, S.R., Kalyan, R. K. Joshi, S., Balai, C. M., Mahla, M. K., & Rokadia, P. (2019). Report of An Exotic Invasive Pest The Fall Armyworm, Spodoptera frugiperda (J.E. Smith) on Maize in Southern Rajasthan. Journal of Entomology and Zoology Studies, 7(3), 1296‒1300. Retrieved from

Bagariang, W., Tauruslina, E., Kulsum, U., Murniningtyas, T., Suyanto, H., Surono, Cahyana, N. A., & Mahmuda, D. (2020). Efektivitas Insektisida Berbahan Aktif Klorantraniliprol terhadap Larva Spodoptera frugiperda (JE Smith) [Effectivity of Chlorantraniliprole Insecticide against the Larva of Spodoptera frugiperda (JE Smith)]. Jurnal Proteksi Tanaman, 4(1), 29‒37.

Barta, M., & Cagáň, L. (2006). Aphid-pathogenic Entomophthorales (Their Taxonomy, Biology and Ecology). Biologia, 61(Suppl 21), S543‒S616.

Centre for Agriculture and Bioscience International. (2016). Spodoptera frugiperda (Fall Army Worm). Datasheet. Invasive Species Compendium.

Dannon, H. F., Dannon, A. E., Douro-Kpindou, O. K., Zinsou, A. V., Houndete, A. T., Toffa-Mehinto, J., Elegbede, I. A. T. M., Olou, B. D., & Tamo, M. (2020). Toward the Efficient Use of Beauveria bassiana in Integrated Cotton Insect Pest Management. Journal of Cotton Research, 3(1), 24.

Dara, S. K. (September 25, 2017). Entomophthora planchoniana Infecting The Strawberry Aphid, Chaetosiphon fragaefolii in California. E-Journal of Entomology and Biologicals. UCANR electronic extension journal previously published as "Strawberries and Vegetables." Retrieved from

da Silva Santos, A. C., Diniz, A. G., Tiago, P.V., & de Oliveira, N. T. (2020). Entomopathogenic Fusarium Species: A Review of Their Potential for the Biological Control of Insects, Implications and Prospects. Fungal Biology Reviews, 34(1), 41‒57.

de H. C. Maciel, M., do Amaral, A. C. T., da Silva, T. D., Bezerra, J. D. P., de Souza-Motta, C. M., da Costa, A. F., Tiago, P. V., & de Oliveira, N. T. (2021). Evaluation of Mycotoxin Production and Phytopathogenicity of the Entomopathogenic Fungi Fusarium caatingaense and F. pernambucanum from Brazil. Current Microbiology, 78(4), 1218‒1226.

‌Diniz, A. G., de Cerqueira, L. V.-B. M. P., de Oliveira Ribeiro, T. K., da Costa, A. F., & Tiago, P. V. (2022). Pathogenicity of Isolates of Fusarium incarnatum-equiseti Species Complex to Nasutitermes corniger (Blattodea: Termitidae) and Spodoptera frugiperda (Lepidoptera: Noctuidae). International Journal of Pest Management, 68(2), 103‒112.

Firake, D.M., & Behere, G.T. (2020). Natural Mortality of Invasive Fall Armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) in Maize Agroecosystems of Northeast India. Biological Control, 148, 104303.

Fitriana, Y., Suharjo, R., Swibawa, I. G., Semenguk, B., Pasaribu, L. T., Hartaman, M., Rwandini, R. A., Indriyati, Purnomo, & Solikhin. (2021). Aspergillusoryzae and Beauveriabassiana as Entomopathogenic Fungi of Spodopteralitura Fabricius (Lepidoptera: Noctuidae) Infesting Corn in Lampung, Indonesia. Egyptian Journal of Biological Pest Control, 31, 127.

Food and Agriculture Organization. (2019). Briefing Note on FAO Action on Fall Armyworm. Rome, Italy: FAO. Retrieved from

Frisvad, J.C., Hubka, V., Ezekiel, C. N., Hong, S.-B., Nováková , A., Chen, A. J., Arzanlou, M., Larsen, T. O., Sklenar, F., Mahakarnchanakul, W., Samson, R. A., & Houbraken, J. (2019). Taxonomy of Aspergillus Section Flavi and Their Production of Aflatoxins, Ochratoxins and Other Mycotoxins. Studies in Mycology, 93(1), 1–63.

Goergen, G , Kumar, P. L., Sankung, S. B., Togola, A., & Tamò, M. (2016). First Report of Outbreaks of the Fall Armyworm Spodoptera frugiperda (J. E. Smith) (Lepidoptera, Noctuidae), a New Alien Invasive Pest in West and Central Africa. PLoS ONE, 11(10), e0165632.

Gutiérrez-Moreno, R., Mota-Sanchez, D., Blanco, C. A., Whalon, M. E., Terán-Santofimio, H., Rodriguez-Maciel, J. C., & DiFonzo, C. (2018). Field-Evolved Resistance of the Fall Armyworm (Lepidoptera: Noctuidae) to Synthetic Insecticides in Puerto Rico and Mexico. Journal of Economic Entomology, 112(2), 792–802.

Humber, R. A. (2012). Identification of Entomopathogenic Fungi. In L. A. Lacey (Ed.), Manual of Techniques in Invertebrate Pathology (2nd ed., pp. 151–187). London, United Kingdom: Academic Press.

Inglis, G. D, Goettel, M. S., Butt, T. M., & Strasser, H. (2001). Use of Hyphomycetous Fungi for Managing Insect Pests. In T. M. Butt, C. W. Jackson, & N. Magan (Eds.), Fungi as Biocontrol Agents, Progress, Problems and Potential (pp. 23–70). London, United Kingdom: CABI Publishing.

International Plant Protection Convention. (2018). First Detection of Fall Army Worm on the Border of Thailand. IPPC Official Pest Report, No. THA-03/1. Rome, Italy: FAO.

International Plant Protection Convention. (2019). The Occurence of Fall Armyworm (Spodoptera frugiperda) in Indonesia. Retrieved from

Klich, M. A., & Pitt, J. I. (1988). A Laboratory Guide to Common Aspergillus Species and Their Teleomorphs. North Ryde, Australia: CSIRO Division of Food Processing.

Lestari, P., Budiarti, A., Fitriana, Y., Susilo, FX., Swibawa, I. G., Sudarsono, H., Suharjo, R., Hariri, A. M., Purnomo, Nuryasin, Solikhin, Wibowo, L., Jumari, & Hartaman, M. (2020). Identification and Genetic Diversity of Spodoptera frugiperda in Lampung Province, Indonesia. Biodiversitas, 21(4), 1670–1677.

Marcinkeviciusa, K., Salvatore, A., Murua, G., Arena, M., & Vera, N. (2019). Phytochemical Investigation and Biological Activities of Fusarium sp. An Entomogenous Fungus. Biocatalysis and Agricultural Biotechnology, 18, 101084.

‌Meyling, N. V., Pell, J. K., & Eilenberg, J. (2006). Dispersal of Beauveria bassiana by the Activity of Nettle Insects. Journal of Invertebrate Pathology, 93(2), 121‒126.

Minarni, E. W, Nurtiati, Suyanto, A., & Suroto, A. (2020a). Penyebaran Hama Invasif Spodoptera frugiperda pada Tanaman Jagung dan Potensi Musuh Alaminya di Kabupaten Banyumas. Paper presented at Seminar Nasional LPPM Universitas Jenderal Soedirman. Purwokerto, Indonesia, October 6‒7, 2020.

Minarni, E. W, Soesanto, L., Suyanto, A., & Rostaman. (2020b). Exploration and Pathogenicity Test of Entomopathogenic Fungus from Brown Planthopper (Nilaparvata lugens Stal) Pest. Ecology, Environment and Conservation, 26(1), 24‒33. Retrieved from

Montezano, D. G., Specht, A., Sosa-Gómez, D. R., Roque-Specht, V. F. & Sousa-Silva, J. C. (2018). Host Plants of Spodoptera frugiperda (Lepidoptera: Noctuidae) in the Americas. African Entomology, 26(2), 286–300.

Muthukrishnan, S., Merzendorfer, H., Arakane, Y., & Kramer, K. J. (2012). Chitin Metabolism in Insects. In L. I. Gilbert (Ed.), Insect Molecular Biology and Biochemistry (1st Edition, pp. 193‒235). London, United Kingdom: Academic Press Elsevier B.V.

Nonci, N., Kalqutny, S. H., Mirsam, H., Muis, A., Azrai, M., & Aqil, M. (2019). Pengenalan Fall Armyworm (Spodoptera frugiperda J. E. Smith) Hama Baru Tanaman Jagung di Indonesia. Maros, Indonesia: Kementerian Pertanian. Badan Penelitian dan Pengembangan Pertanian, Balai Penelitian Tanaman Serealia. Retrieved from

Pavlyushin, V. (2020). Pathogenic Post-Effect of Entomopathogenic Fungi on Phytophagous Pests and Entomophagous Biocontrol Agents. BIO Web Conferences, 21, 00020. XI International Scientific and Practical Conference “Biological Plant Protection is the Basis of Agroecosystems Stabilization”. Krasnodar, Russia,

Prasanna, B. M., Huesing, J. E., Eddy, R., & Peschke, V. M. (Eds.). (2018). Fall Armyworm in Africa: A Guide For Integrated Pest Management. 1st Edition. USAID and CIMMYT, Mexico. Retrieved from

Purnomo, H. (2009). Pengantar Pengendalian Hayati. Yogyakarta, Indonesia: CV. Andi Offset.

Ramadhan, R. A. M., Puspasari, L. T., Meliansyah, R., Maharani, R., Hidayat, Y. & Dono, D. (2016). Bioaktivitas Formulasi Minyak Biji Azadirachta indica (A. Juss) terhadap Spodoptera litura F. [Bioactivity Formulation of Seed Neem Oil Azadirachta indica (A. Juss) against Spodoptera litura F.] Jurnal Agrikultura, 27(1), 1–8.

Rosmiati, A., Hidayat, C., Firmansyah, E. & Setiati, Y.(2018). Potensi Beauveria bassiana sebagai Agens Hayati Spodoptera litura Fabr. pada Tanaman Kedelai. Jurnal Agrikultura, 29(1), 43–47.

Sari, M., Lubis, L. & Pangestiningsih, Y. (2013). Uji Efektivitas Beberapa Insektisida Nabati untuk Mengendalikan Ulat Grayak (Spodoptera litura F.) (Lepidoptera: Noctuidae) di Laboratorium. Jurnal Online Agroteknologi, 1(3), 560–569. Retrieved from

Seye, F., Bawin, T., Boukraa, S., Zimmer, J.-Y., Ndiaye, M., Delvigne, F., & Francis, F. (2014). Pathogenicity of Aspergillus clavatus Produced in a Fungal Biofilm Bioreactor toward Culex quinquefasciatus (Diptera: Culicidae). ). Journal of Pesticide Science, 39(3), 127–132.

Sharanabasappa, Kalleshwaraswamy, C. M., Maruthi, M. S., & Pavithra, H. B. (2018). Biology of Invasive Fall Army Worm Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) on Maize. Indian Journal of Entomology, 80(3), 540–543.

Singh, D., Rainab, T. K. & Singh, J. (2017). Entomopathogenic Fungi: An Effective Biocontrol Agent for Management of Insect Populations Naturally. Journal of Pharmaceutical Sciences and Research, 9(6), 830-839. Retrieved from

Thomsen, L., Bresciani, J., & Eilenberg, J. (2001). Formation and Germination of Resting Spores from Different Strains from the Entomophthora muscae Complex Produced in Musca domestica. Canadian Journal of Botany, 79(9), 1076‒1082.

Trisyono, Y. A., Suputa, Aryuwandari, V. E. F., Hartaman, M., & Jumari. (2019). Occurrence of Heavy Infestation by the Fall Armyworm Spodoptera frugiperda, a New Alien Invasive Pest, in Corn in Lampung Indonesia. Jurnal Perlindungan Tanaman Indonesia, 23(1), 156–160.

Varga, J., Frisvad, J. C., Kocsubé, S., Brankovics, B., Tóth, B., Szigeti, G., & Samson, R. A. (2011). New and Revisited Species in Aspergillus section Nigri. Studies in Mycology, 69(1), 1–17.

Vega, F. E., Meyling, N. V., Luangsa-ard, J. J., & Blackwell, M. (2012). Fungal Entomopathogens. In F. E. Vega & H. K. Kaya (Eds.), Insect Pathology (2nd Edition, pp. 171–220). London, United Kingdom: Academic Press.

Wigglesworth, V. B. (1974). The Principles of Insect Physiology. 7th Edition. London, United Kingdom: Chapman and Hall.

Zekeya, N., Ndakidemi, P.A., Chacha, M. & Mbega, E. (2017). Tomato Leafminer, Tuta absoluta (Meyrick 1917), an Emerging Agricultural Pest in Sub-Saharan Africa: Current and Prospective Management Strategies. African Journal of Agricultural Research, 12(6), 389–396.

Zhang, P., You, Y., Song, Y., Wang, Y, & Zhang, L. (2015). First Record of Aspergillus oryzae (Eurotiales: Trichocomaceae) as An Entomopathogenic Fungus of the Locust, Locusta migratoria (Orthoptera: Acrididae). Biocontrol Science and Technology, 25(11), 1285‒1298.


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