* Corresponding Author
Potency of Yeasts to Promote Induced Resistance on Chili Plant (Capsicum annuum L.) against Yellow Leaf Curl Disease
https://doi.org/10.22146/jpti.52089 Sri Hartati(1*), Rika Meliansyah(2), Luciana Djaya(3), Ceppy Nasahi(4), Ramadhan Kamal Putra(5)
(1) Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran Jln. Raya Bandung-Sumedang, Jatinangor, West Java 45363
(2) Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran Jln. Raya Bandung-Sumedang, Jatinangor, West Java 45363
(3) Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran Jln. Raya Bandung-Sumedang, Jatinangor, West Java 45363
(4) Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran Jln. Raya Bandung-Sumedang, Jatinangor, West Java 45363
(5) Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran Jln. Raya Bandung-Sumedang, Jatinangor, West Java 45363
(*) Corresponding Author
Abstract
Yellow leaf curl is a major disease on chili plants. The use of antagonistic yeasts as the control agents is a good alternative in an environmentally friendly control method. This study was objected to evaluate the potencies of Rhodotorula minuta and Candida tropicalis to promote induced resistance on chili plants against yellow leaf curl disease. The experiment was arranged in the randomized complete block design with 9 treatments and 3 replications. The treatments were the application of the yeasts at three different times of virus inoculation, as follows: A. R. minuta, virus inoculation at 3 days after transplanting (dat), B. R. minuta, virus inoculation at 7 dat, C. R. minuta, virus inoculation at 10 dat, D. C. tropicalis, virus inoculation at 3 dat, E. C. tropicalis, virus inoculation at 7 dat, F. C. tropicalis, virus inoculation at 10 dat, G. control, virus inoculation at 3 dat, H. control, virus inoculation at 7 dat, and I. control, virus inoculation at 10 dat. No treatment was applied to the control. The yeasts were applied by soaking the chili seeds, and pouring the suspension into the growth media at transplanting. The variables observed were incubation period, disease severity, and disease incidence. Peroxidase activity, phenylalanine ammonia lyase activity, and salicylic acid accumulation were also analyzed. The results showed that the application of R. minuta or C. tropicalis was able to extend the disease incubation period, but did not reduce the yellow leaf curl disease incidence and severity. The treatment of R. minuta, virus inoculation at 7 dat, increased the peroxidase activity from 2590.80 units to 6870.93 units (0.5 minute) and from 577.367 units to 1131.300 units (2.5 minutes), PAL activity from 16.059 to 17.911 A290/mg, and accumulation of salicylic acid from 2.785 to 6.263 ppm. Application of C. tropicalis, virus inoculation at 7 dat, increased the peroxidase activity from 2590.80 units to 6033.067 units (0.5 minute) and from 577.367 units to 950.967 units (2.5 minutes), and accumulation of salicylic acid from 2.785 to 6.982 ppm.
Keywords
Full Text:
PDFReferences
Adilah, N.F. & S.H. Hidayat. 2014. Keparahan Penyakit Daun Keriting Kuning dan Pertumbuhan Populasi Kutukebul pada Beberapa Genotipe Cabai. Jurnal Fitopatologi Indonesia 1: 195–201.
Al-Ani, R.A., M.A. Athab & O.N. Matny. 2013. Management of Potato Virus Y (PVY) in Potato by Some Biocontrol Agensts under Field Conditions. Environmental Biology 7: 441–444.
Chan, Z., & S. Tian. 2007. Proteom Approach to Characterize Proteins Induced by Antagonist Yeast and Salicylic Acid in Peach Fruit. Journal of Proteome Research 6: 1677–1688.
Cooke, B.M. 2006. Disease Assessment and Yield Loss, p. 43–80. In B.M. Cooke, D.G. Jones, & B. Kaye (eds.), The Epidemiology of Plant Diseases. Springer, Netherlands.
El Ghaouth, A., C.L. Wilson, & M. Wisniewski. 2003. Control of Postharvest Decay of Apple Fruit with Candida saitoana and Induction of Defense Responses. Phytopathology 93: 344–348.
El-Mougy, N.S., M.M. Abdel-Kader., S.M. Lashin., & A.A. Megahed. 2013. Fungicides Alternatives as Plant Resistance Inducers against Foliar Diseases Incidence of Some Vegetables Grown under Plastic Houses conditions. International Journal of Engineering and Innovative Technology (IJEIT) 3: 71–81 .
Faizah, R., S. Sujiprihati, M. Syukur, & S.H. Hidayat. 2012. Ketahanan Biokimia Tanaman Cabai terhadap Begomovirus Penyebab Penyakit Daun Keriting Kuning. Jurnal Fitopatologi Indonesia 8: 138–144.
Gautam, P., & J. Stein. 2011. Induction of Systemic Acquired Resistance to Puccinia sorghi in Corn. International Journal of Plant Pathology 2: 43–50.
Gunaeni, N., A.W. Wulandari, & A. Hudayya. 2015. Pengaruh Bahan Ekstrak Tanaman terhadap Pathogenesis Related Protein dan Asam Salisilat dalam Menginduksi Resistensi Tanaman Cabai Merah terhadap Virus Kuning Keriting. Jurnal Hortikultura 25: 160–170.
Habibullah, M., A. Widiastuti, & C. Sumardiyono. 2018. Respons Awal Ketahanan Jagung terhadap Peronosclerospora maydis dan Induksi Bahan Kimia. Jurnal Perlindungan Tanaman Indonesia 22: 27–32.
Hartati, S. 2016. Khamir sebagai Agens Biokontrol Antraknosa (Colletotrichum acutatum) pada Tanaman Cabai Pascapanen. Disertasi. Institut Pertanian Bogor, Bogor. 94 p.
Hartati, S., L. Tarina, E. Yulia, & L. Djaya. 2019a. Induksi Resistensi dengan Rhodotorula minuta untuk Mengendalikan Antraknosa (Colletotrichum acutatum J. H. Simmonds) pada Tanaman Cabai. Jurnal Agrikultura 30: 91–99.
Hartati, S., L. Tarina, E. Yulia, & L. Djaya. 2019b. Pengaruh Induksi Resistensi oleh Khamir Candida tropicalis terhadap Pertumbuhan Tanaman Cabai Terinfeksi Colletotrichum acutatum. Jurnal Agrikultura 30: 17–24.
Hershkovitz, V., C. Ben-Dayan, G. Raphael, M. Pasmanik-Chor, J. Liu, E. Belausov, R. Aly, M. Wisniewski, & S. Droby. 2012. Global Changes in Gene Expression of Grapefruit Peel Tissue in Response to the Yeast Biocontrol Agent Metschnikowia fructicola. Molecular Plant Pathology 13: 338–349.
Hidayat, S.H., O. Chatchawankanpanich, E. Rusli, & N. Aidawati. 2006. Begomovirus Associated with Pepper Yellow Leaf Curl Disease in West Java, Indonesia. Journal Indonesia Microbiology 11: 87–89.
Hindumathy, C.K. 2012. The Defense Activator from Yeast for Rapid Induction of Resistance in Suseptible Pearl Millet Hybrid Against Downy Mildew Disease. International Journal of Agriculture Sciences 4: 196–201.
Kloepper, J.W., S. Tuzun , & J.A. Kuc. 1992. Proposed Definitions Related to Induced Disease Resistance. Biocontrol Science and Technology 2: 349–351.
Lisker, N., L. Cohen, E. Chalutz, & Y. Fuchs. 1983. Fungal Infections Suppress Ethylene Iinduced Phenylalanine ammonia-lyase Activity in Grapefruit. Physiology Plant Pathology 22: 331–338.
Murphy, A.M., A. Gilliand, C.E. Wong, J. West, D.P. Shingh, & J.P. Carr. 2001. Signal Transduction in Resistance to Plant Viruses. European Journal Plant Pathology 107: 121–128.
Namdeo, A.G. 2007. Plant Cell Elicitation for Production of Secondary Metabolites: a review. Pharmacognosy Reviews 1: 69–79.
Narusaka, M., T. Minami, C. Iwabuchi, T. Hama-saki, S. Takasaki, K. Kawamura, & Y. Narusaka. 2015. Yeast Cell Wall Extract Induces Disease Resistance Against Bacterial and Fungal Pathogens in Arabidopsis thaliana and Brassica Crop. PLoS One 10: 1–14.
Naylor, M., A.M. Murphy, J.O. Berry, & J.P. Carr. 1998. Salicylic Acid Can Induce Resistance to Plant Virus Movement. Molecular Plant Microbe Interaction 11: 860–866.
Olivera, M.D.M., C.M.R. Varanda, & M.R.F. Félix. 2016. Induced Resistance During the Interaction Pathogen X Plant and the Use of Resistance Inducers. Phytochemistry Letters reviews 15: 152–158.
Ouchi, S. 1983. Induction of Resistance or Susceptibility. Annual Review Phytopathology 21: 289–315.
Park, K.S., & J.W. Kloepper. 2000. Activation of PR-1a Promoter by Rhizobacteria that Induce Systemic Resistance in Tobacco against Pseudomonas syringae pv tabaci. Biological Control 18: 2–9.
Rusli, E.S., S.H. Hidayat, R. Suseno, & B. Tjahjono. 1999. Virus Gemini pada Cabai: Variasi Gejala dan Studi Cara Penularan. Buletin Hama dan Penyakit Tumbuhan 11: 26–31.
Shan, C.H., W. Chen, H. Zhang, F.-X. Tang, & J.-M. Tong. 2014. Effecton Antagonistic Yeast XL-1 on Resistance-Associated Enzyme Activities in Postharvest Cantaloupe. Genetics and Molecular Research 13: 6253–6258.
Sharma, R.R., D. Singh, & R. Singh. 2009. Biological Control of Postharvest Diseases of Fruits and Vegetables by Microbial Antagonists. Biological Control 50: 205–221.
Sharman, N. & P.S. Madhu. 2011. Antagonistic Activity of Fructoplane Yeast against Ulocladium Rot of Papaya. Journal of Ornamental and Horticultural Plants 2: 169–182.
Simons, T.J., & A.F. Ros. 1970. Enhanced Peroxidase Activity Associated with Induction of Resistance to Tobacco mosaic virus in Hypersensitive Tobacco. Phytopathology 60: 383–384.
Talarczyk, A., M. Krzymowska, W. Borucki, & J. Hennig. 2002. Effect of Yeast CTA1 Gene Expression on Response of Tobacco Plants to Tobacco Mosaic Virus Infection. Plant Physiology 129: 1032–1044.
Tanhaken, R., & C. Rubel. 1997. Salicylic Acid Needed in Hypersensitive Cell Death in Soybean but does not Act a Catalase Inhibitor. Plant Physiology 115: 291–298.
Taufik, M., A. Rahman, A. Wahab, & S.H. Hidayat. 2010. Mekanisme Ketahanan Terinduksi oleh Plant Growth Promotting Rhizobacteria (PGPR) pada Tanaman Cabai Terinfeksi Cucumber mosaic virus (CMV). Jurnal Hortikultura 20: 274–283.
Trisno, J., S.H. Hidayat, & I. Manti. 2010. Hubungan Strain Geminivirus dan Serangga Vektor Bemisia tabaci dalam Menimbulkan Penyakit Kuning Keriting Cabai. Manggoro 11: 1–7.
Walters, D., D. Walsh, A. Newton, & G. Lyon. 2005. Induced Resistance for Plant Disease Control: Maximizing the Efficacy of Resistance Elicitors. Phytopathology 95:1368–1373.
Yao, H.J., & S.P. Tian. 2005. Effects of a Biocontrol Agent and Methyl Jasmonate on Postharvest Diseases of Peach Fruit and the Possible Mechanisms Involved. Journal of Apllied Microbiology 98: 941–950.
DOI: https://doi.org/10.22146/jpti.52089
Article Metrics
Abstract views : 2220
| 
views : 1313
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics