Respons Awal Ketahanan Jagung terhadap Peronosclerospora maydis dan Induksi Bahan Kimia

https://doi.org/10.22146/jpti.26877

Muhammad Habibullah(1), Ani Widiastuti(2*), Christanti Sumardiyono(3)

(1) Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author

Abstract


Downy mildew is an important disease in maize cultivation in the world. Induced resistance is one of the methods used to control plant diseases. Reactive oxygen species (ROS) and lignification are plant defense responses. This study aims to determine the potential of chemicals as an inducer by observing ROS responses and lignification of mycelium. The materials used are benzoic acid, sodium benzoate, salicylic acid, thiamine, saccharin and aspirin with concentration 2 g L-1 of distilled water. The ROS reaction is indicated by all treatments induced and inoculated by pathogens. Lignification of mycelium occurred in the treatment of aspirin at 6 hours observation after inoculation and saccharin treatment on observation 12 hours after inoculation. Based on the observation of ROS and lignification of the mycelium, it is suspected that the material tested has the potential to be further tested as an inducer because it has the ability to activate an early marker of plant resistance in the form of ROS reaction and lignification of mycelium.

 

Intisari

Bulai merupakan penyakit penting dalam budidaya jagung di dunia. Induksi ketahanan adalah salah satu metode yang digunakan untuk mengendalikan penyakit tanaman. Spesies oksigen reaktif (ROS) dan lignifikasi adalah respon pertahanan tanaman. Penelitian ini bertujuan untuk mengetahui potensi bahan kimia sebagai bahan penginduksi dengan mengamati respons ROS dan lignifikasi miselium. Bahan yang digunakan adalah asam benzoat, natrium benzoat, asam salisilat, tiamin, sakarin dan aspirin dengan konsentrasi 2 g akuades L-1. Reaksi ROS ditunjukkan oleh semua perlakuan yang diinduksi dan diinokulasi oleh patogen. Lignifikasi miselium terjadi pada perlakuan aspirin pada pengamatan 6 jam setelah inokulasi dan perlakuan sakarin pada pengamatan 12 jam setelah inokulasi. Berdasarkan pengamatan ROS dan lignifikasi miselium, diduga bahwa bahan yang diuji memiliki potensi untuk diuji lebih lanjut sebagai bahan penginduksi karena memiliki kemampuan untuk mengaktifkan penanda awal ketahanan tanaman dalam bentuk reaksi ROS dan lignifikasi miselium.


Keywords


induced resistance; induksi ketahanan; lignification of mycelium; lignifikasi miselium; maize downy mildew; penyakit bulai jagung; ROS

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References

Abdel-Monaim, M. F. 2011. Role of Riboflavin and Tiamin in Induce Resistance against Charcoal Rot Disease of Soybean. African Journal of Biotechnology 10: 10842−10855.

Agrios. G.N. 2005. Plant Pathology. 5th edition. Academic Press, San Diego. 629 p.

Aleandri, M.P., R. Reda., V. Tagliavetnto., P. Magro, & G. Chilosi. 2010. Effect of Chemical Resistance Inducers on the Control of Monosporascus Root Rot and Vine Decline of Melon. Phytopathologia Mediterranea 49: 18–26.

Amawati, L. 2016. Induksi Ketahanan Jagung terhadap Penyakit Bulai dengan Jamur Trichoderma spp.. Tesis. Fakultas Pertanian Universitas Gadjah Mada, Yogyakarta. 62 hlm.

Boubakri, H., M.A.Wahab., J. Chong., C. Bertsch., A. Mliki, & I. Soustre-Gacougnolle. 2012. Tiamine Induced Resistance to Plasmopara viticola in Grapevine and Elicited Host-Defense Responsses, Including HR Like-cell Death. Plant Physiology and Biochemistry 57: 120−133.

Boyle, C., & D. Walters. 2005. Induction of Systemic Protection against Rust Infection in Broad Bean by Saccharin: Effects on Plant Growth and Development. New Phytologist 167: 607−612.

Chisholm, S.T., G. Coaker., B. Day, & B.J. Staskawicz. 2006. Host-Microbe Interactions: Shaping the Evolution of the Plant Immune Responsse. Cell 124: 803–814.

Daudi, A., & J.A. O’Brien. 2012. Detetion of Hydrogen Peroxidase by DAB Staining in Arabidopsis Leaves. Bio-protocol 2: 1−4.

Daudi, A., Z. Cheng., J. A. O’Brien., N. Mammarella., S. Khan., F. M. Ausubel, & G. P. Bolwell. 2012. The Apoplastic Oxidative Burst Peroxidase in Arabidopsis is a Major Component of Pattern-Triggered Immunity. The Plant Cell 24: 275–287.

Fadel, F., M. El-Naggar., S, Tolba, & G. Farahat. 2006. Induction of Disease Resistance by Salicylic Acid, Sodium Benzoat and Potassium Mono-phosphate against Ustilago maydis in Maize Plants, p. 240−250. In G.J. Kövics & I. Dávid, Proceeding of  4th International Plant Protection Symposium at Debrecen University. Debrecen, Hungary, October 18−19, 2006.

He, C.Y., T. Hsiang, & D.J. Wolyn. 2002. Induction of Systemic Disease Resistance and Pathogen Defence Responsses in Asparagus officinalis Inoculated with Nonpathogenic Strains of Fusarium oxysporum. Plant Pathology 51: 225−230.

Jonathan, D., G. Jones, & J.L. Dangl. 2006. The Plant Immune System. Nature 444: 323–329.

Kumar, A.,  R. Singh, & B.L. Jalali. 2012. Management of Stem Rot of Rice with Resistance Inducing Chemicals and Fungicides. Indian Phytopathology 56: 226−269.

Li, B., X. Meng., L. Shan, & P. He. 2016. Transcriptional Regulation of Pattern-Triggered Immunity in Plants. Cell Host Microbe 19: 641–650.

Mandal, S., N. Mallick, & A. Mitra. 2009. Salicylic Acid-induced Resistance to Fusarium oxysporum f.sp. lycopersici in Tomato. Plant Physiology and Biochemistry 47: 642–649.

Nicholson, R.L. & R. Hammerschmidt. 1992. Phenolic Compound and their Role in Disease Resistance. Annual Review of Phytopathology 30: 369−389.

Okubara, P.A & T.C. Paulitz, 2005. Root Defense Responses to Fungal Pathogens: A Molecular Perspective. Plant and Soil 274: 215–226.

Oostendorp, M., W. Kunz., B. Dietrich, & T. Staub. 2001. Induced Resistance in Plants by Chemicals. European Journal Plant Pathology 107: 19–28.

Sass, J. 1958. Botanical Microtechnique. 3rd Edition. The Iowa State College Press, Ames, Iowa. 228 p.

Senaratna, T., D. Touchell., E. Bunn, & K. Dixon. 2000. Acetyl Salicylic Acid (Aspirin) and Salicylic Acid Induce Multiple Stress Tolerance in Bean and Tomato Plants. Plant Growth Regulation 30: 157–161.

Sharma, P., A.B. Jha., R.S. Dubey, & M. Pessarakli. 2012. Reactive Oxygen Species, Oxidative Damage, and Antioxidative Defense Mechanism in Plants under Stressful Conditions. Journal of Botany 2012: 1−27.

Siegrist, J., D. Glenewinkel, C. Kolle, & M. Schmidke. 1997. Chemically Induced Resistance in Green Bean against Bacterial and Fungal Pathogens. Journal of Plant Diseases and Protection 104: 599–610.

Sumardiyono, C. 1991. Mekanisme Ketahanan Kopi Arabika terhadap Penyakit Karat Daun (Hemileia vastatrix). Disertasi. Fakultas Pertanian Universitas Gadjah Mada. Yogyakarta. 179 hlm.

Sumardiyono, C. 2008.  Ketahanan Jamur terhadap Fungisida di Indonesia. Jurnal Perlindungan Tanaman Indonesia 14: 1−5.

Sumardiyono, C., Suharyanto, Suryanti, P. Rositasari, & Y.D. Chinta. 2015. Deteksi Penginduksian Ketahanan Pisang terhadap Penyakit Layu Fusarium dengan Asam Fusarat. Jurnal Perlindungan Tanaman Indonesia 19: 40–44.

Van Loon, L.C. & E.A. Van Strien. 1999. The Families of Pathogenesis-Related Proteins, Their Activities, and Comparative Analysis of Pr-1 Type Proteins. Physiological and Molecular Plant Pathology 55: 85–97.

White, R.F. 1979. Acetylsalicylic Acid (Aspirin) Induces Resistance to Tobacco mosaic virus in Tobacco. Virology 99: 410–412.

Widiastuti, A., M. Yoshino., H. Saito., K. Maejima., S. Zhou, H. Odani, K. Narisawa, M. Hasegawa, Y. Nitta, & T. Sato. 2013a. Heat Shock-induced Resistance in Strawberry against Crown Rot Fungus Colletotrichum gloeosporioides. Physiological and Molecular Plant Pathology 84: 86−91.

Widiastuti, A., M. Yoshino, H. Saito, K. Maejima, S. Zhou, H. Odani, K. Narisawa, M. Hasegawa, Y. Nitta, & T. Sato. 2011. Induction of Disease Resistance against Botrytis cinerea by Heat Shock Treatment in Melon (Cucumis melo L.). Physiological and Molecular Plant Pathology 75: 157-162.

Widiastuti, A., M. Yoshino, M. Hasegawa, Y. Nitta, & T. Sato. 2013b. Heat Shock-induced Resistance Increases Chitinase-1Gene Expression and Stimulates Salicylic Acid Production in Melon (Cucumis melo L.). Physiological and Molecular Plant Pathology 82: 51−55.

Yuniasih, D.A. 2014. Ketahanan Peronosclerospora maydis terhadap Fungisida. Tesis. Fakultas Pertanian Universitas Gadjah Mada, Yogyakarta. 64 hlm.



DOI: https://doi.org/10.22146/jpti.26877

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