* Corresponding Author
Potato Cyst Nematode in East Java: Newly Infected Areas and Identification
https://doi.org/10.22146/jpti.25498 Happy Cahya Nugrahana(1*), Siwi Indarti(2), Edhi Martono(3)
(1) Agricultural Quarantine Major Service of Surabaya Jln. Ir. H. Juanda, Semambung, Gedangan, Sidoarjo, East Java 61253
(2) Department of Crop Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Crop Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
Potato Cyst Nematodes (PCN), Globodera rostochiensis has noted to be a devastated pest on potato in Indonesia. It is listed as the A2 pest by Plant Quarantine of Republik Indonesia, and it was also being a highly concerned plant parasitic nematode species worlwide. Therefore, both intensive and extensive surveys should be done to monitor the spread of PCN, especially in East Java as one of the centre of potato plantations in Indonesia. The aim of this study was to study the distribution of PCN in four potato plantations in East Java, i.e. Batu, Magetan, Probolinggo, and Pasuruan which were located between 1,205 to 2,063 m above the sea level. Extraction and isolation of cysts from soil samples was done using Baunacke method, and it was followed by identification of the nematodes using morphological and molecular approaches according to Baldwin and Mundo-Ocampo. The results showed that PCN was found on all sampling sites, i.e. Batu (Sumber Brantas, Jurang Kuali, Tunggangan, Junggo, Brakseng); Magetan (Dadi, Sarangan, Singolangu); Probolinggo (Tukul, Pandansari, Ledokombo, Sumberanom, Wonokerto, Ngadas), Pasuruan (Wonokerto, Tosari, Ledoksari, Ngadiwono). Magetan and Pasuruan were noted as new infested areas in East Java. Both morphological and molecular methods showed that the species found on all sites was Globodera rostochiensis.
Intisari
Nematoda Sista Kentang (NSK), Globodera rostochiensis telah tercatat sebagai hama yang menghancurkan tanaman kentang di Indonesia. NSK terdaftar sebagai Organisme Pengganggu Tumbuhan Karantina golongan A2 oleh Badan Karantina Pertanian Republik Indonesia, dan juga merupakan spesies nematoda parasit tanaman yang sangat merugikan di seluruh dunia. Oleh karena itu, baik survei intensif maupun ekstensif harus dilakukan untuk memantau penyebaran NSK, terutama di Jawa Timur sebagai salah satu sentra tanaman kentang di Indonesia. Tujuan dari penelitian ini adalah untuk mempelajari distribusi NSK pada empat daerah sentra penanaman kentang di Jawa Timur, yaitu Batu, Magetan, Probolinggo, dan Pasuruan yang terletak antara 1.205 sampai 2.063 m di atas permukaan laut. Ekstraksi dan isolasi sista NSK dari sampel tanah dilakukan dengan metode Baunacke, dan dilanjutkan dengan identifikasi secara morfologi dan molekuler menurut Baldwin dan Mundo-Ocampo. Hasil penelitian menunjukkan bahwa NSK ditemukan di semua lokasi pengambilan sampel, yaitu Batu (Sumber Brantas, Jurang Kuali, Tunggangan, Junggo, Brakseng); Magetan (Dadi, Sarangan, Singolangu); Probolinggo (Tukul, Pandansari, Ledokombo, Sumberanom, Wonokerto, Ngadas), Pasuruan (Wonokerto, Tosari, Ledoksari, Ngadiwono). Magetan dan Pasuruan tercatat sebagai daerah sebaran baru di Jawa Timur. Hasil identifikasi secara morfologi dan molekuler menunjukkan bahwa spesies yang ditemukan di semua lokasi adalah Globodera rostochiensis.
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DOI: https://doi.org/10.22146/jpti.25498
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