Keragaman Genetik Metarhizium anisopliae dan Virulensinya pada Larva Kumbang Badak (Oryctes rhinoceros)

https://doi.org/10.22146/jpti.16015

Aisyah Surya Bintang(1*), Arif Wibowo(2), Tri Harjaka(3)

(1) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(2) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jln. Flora 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author

Abstract


Rhinoceros beetle (Oryctes rhinoceros) is one of the important pests of coconut tree. One of eco-friendly control applied for this pest is by using entomopathogenic fungiMetarhizium anisopliae. There is not much information about the variability and virulence of M. anisopliae toward O. rhinoceros. M. anisopliae isolates obtained from Biological Control Laboratory, Faculty of Agriculture, Universitas Gadjah Mada were cultured on PDA medium.M. anisopliae isolates was isolated from O. rhinoceros larvae (MaOr), Lepidiota stigma larvae (MaLs), Brontispa longissima beetle (MaBl).O. rhinoceros beetles were obtained from Kulon Progo, DIY. This study used molecular test, and virulence test toward 3rd stadium of O. rhinoceros larvae by using dipping method. Molecular test by sequence and phylogenetic analysis, showed that MaOr was located at different group (out group) with MaLs and MaBr. On the density 107 conidium/ml MaOr and MaLs were more virulent than MaBl towards 3rd stadium of O. rhinoceros larvae.


INTISARI

Kumbang badak (Oryctes rhinoceros) merupakan salah satu hama penting pada tanaman kelapa. Salah satu upaya pengendalian yang ramah lingkungan adalah dengan menggunakan jamur entomopatogen, yakni Metarhizium anisopliae. Belum banyak diketahui mengenai keragaman dan juga virulensi dari M. anisopliae terhadap O. rhinoceros. Tujuan dari penelitian ini adalah untuk mengetahui keragaman genetik M. anisopliae dan virulensinya pada larva kumbang badak. Isolat yang digunakan berasal dari Laboratorium Pengendalian Hayati, Fakultas Pertanian, Universitas Gadjah Mada dalam bentuk kultur murni pada medium PDA. Isolat yang gunakan diisolasi dari larvaOryctes rhinoceros (MaOr), larva Lepidiota stigma (MaLs), dan kumbang Brontispa longissima (MaBl). Serangga yang diuji berasal dari daerah Kulon Progo, DIY. Pengujian secara molekuler dengan analisis sekuensing dan filogenetik, menunjukkan bahwa isolat MaOr terletak pada grup yang berbeda dengan MaLs dan MaBl berdasarkan pada urutan basa DNA. Pada kerapatan 107 konidium/ml isolat MaOr dan MaLs lebih virulen terhadap larva O. rhinoceros instar 3 dibandingkan dengan MaBl.


Keywords


genetic diversity; keragaman genetik; Metarhizium anisopliae; Oryctes rhinoceros; virulence; virulensi

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DOI: https://doi.org/10.22146/jpti.16015

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