* Corresponding Author
Eksplorasi Bakteri yang Berpotensi sebagai Agens Pengendali Hayati Fusarium solani dan Meloidogyne incognita pada Lada
https://doi.org/10.22146/jpti.15608 Citra Mayang Wardhika(1), Suryanti Suryanti(2*), Tri Joko(3)
(1) Jurusan Hama dan penaykit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada
(2) Jurusan Hama dan penaykit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada
(3) Jurusan Hama dan penaykit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Indonesia is major producer of black pepper (Piper nigrum L.), however the pepper production has been decreasing in the last decades. Black pepper yellowings caused by Fusarium solani and Meloidogyne incognita is one of the most important disease on pepper causing the decrease of pepper production. This research was aimed at the selection of potential bacteria as a biological control agents of F. solani and M. incognita on black pepper. The bacteria were isolated from rhizospheric soil of healthy plant. To determine the ability of biological agents, they were tested against F. solani and M. incognita. Seven isolates fluorescent pseudomonads, 19 isolates of Bacillus spp. and 21 bacterial isolates which were yet to be identified were isolated from soil rhizosphere. The results show that there are 5 antagonist bacterial isolates which were able to inhibit the growth of F. solani but so far no bacteria that caused cell lysis to M. incognita larvae was found.
Indonesia merupakan negara produsen lada yang pada beberapa waktu terakhir ini telah mengalami penurunan
produksi. Penyakit kuning yang disebabkan oleh Fusarium solani dan Meloidogyne incognita merupakan salah satu penyebab terjadinya penurunan tersebut. Penelitian bertujuan untuk menyeleksi bakteri yang berpotensi sebagai pengendali hayati Fusarium solani dan Meloidogyne incognita pada lada. Isolasi bakteri dilakukan dari tanah rizosfer pertanaman lada sehat dan selanjutnya untuk mengetahui kemampuan agens hayati dilakukan uji antagonis terhadap F. solani dan M. incognita. Hasil isolasi dari rizosfer pertanaman didapatkan 7 isolat bakteri kelompok Pseudomonad fluoresen, 19 isolat bakteri Bacillus spp. dan 21 isolat bakteri yang belum diidentifikasi lebih lanjut. Hasil uji antagonis menunjukkan bahwa ada 5 isolat bakteri yang mampu menghambat pertumbuhan F. solani namun belum ditemukan adanya bakteri yang mampu menghambat pertumbuhan M. incognita.
Keywords
Full Text:
PDFReferences
Ajilogba, C.F., O.O. Babalola, & F. Ahmad. 2013. Antagonistic Effects of Bacillus Species in Biocontrol of Tomato Fusarium Wilt. Ethno Medicine 7: 205−216.
Anonim. 2013. IPC Market Review 2013. , diakses 30/5/14.
Arwiyanto, T., F. Yuniarsih, T. Martoredjo, & G. Dalmadiyo. 2007. Seleksi Pseudomonad fluoresen secara Langsung di Lapangan untuk Pengendalian Penyakit Lincat pada Tembakau. Jurnal Hama Penyakit Tumbuhan Tropika 7: 62–68.
Ashoub, A.H. & M.T. Amara. 2010. Biocontrol Activity of Some Bacterial Genera against Rootknote Nematode, Meloidogyne incognita. Journal of American Science 6: 321−328.
Cazorla, F.M., D. Romero, A.Perez-Garcia, B.J.J. Lugtenberg, A. de Vicente, & G. Bloemberg. 2007. Isolation and Characterization of Antagonistic Bacillus subtilisStrains from the Avocado Rhizoplane Displaying Biocontrol Activity. Journal of Applied Microbiology 103: 1950–1959.
Choudhary, D.K. & B.N. Johri. 2008. Interaction of Bacillus spp. and Plants-with Special Reference to Induced Systemic Resistance (ISR). <http://www.sciencedirect.com.science>, diakses 28/2/09.
Danya, M.K. & V.P. Potty. 2007. Sideriphore Production by Pseudomonas fluorescens isolated from rhizosphere of Solestemon rotundifolius. Journal of Root Crop 33: 138−140.
Haas D, & G. Defago. 2005. Biological Control of Soil-borne Pathogens by Fluorescent Pseudomonads. Natural Review of Microbiology 3: 307−319.
Haggag, W.M. & H.A.A. Mohamed. 2007. Biotechnological Aspects of Microorganism Used in Plant Biological Control. Am-Eurasian Journal Sustainable Agriculture 1: 7−12.
Karkachi, N.E., S., Gharbi, M., Kihal, & J.E., Henni. 2010. Biological Control of Fusarium oxysporum f.sp. lycopersici Isolated from Algerian Tomato by Pseudomonas fluorescens, Bacillus careus, Serratia marcescens and Trichoderma harzianum. Research Journal of Agronomy 4: 31−34.
Lamovšek, J., G. Urek, & S. Trdan. 2013. Biological Control of Root-Knot Nematodes (Meloidogyne spp.): Microbes against the Pests. Acta agriculturae Slovenica 101: 263−275.
Nagorska, K., M. Bilowski & M. Obuchowski. 2007. Multicelluler Behaviour and Production of Wide Variety of Toxic Substances Support Usage of Bacillus subtilis as Powerful Biocontrol Agent. Acta Biochimica Polonica 54: 495−508.
Naik, P.R., G. Raman, K.B. Narayanan, & N. Sakthivel.. 2008. Assessment of Genetic and Functional Diversity of Phosphate Solubilizing Fluorescent Pseudomonads. Biomedical Central Microbiology 8: 230.
Nourozian, J., H.R., Etebarian, &G. Khodakaramian. 2006. Biological Control of Fusarium graminearum on Wheat by Antagonistic Bacteria Songklanakarin Journal of Science and Technology 28: 29−38.
DOI: https://doi.org/10.22146/jpti.15608
Article Metrics
Abstract views : 6871
| 
views : 3603
Refbacks
- There are currently no refbacks.
Copyright (c) 2014 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics