Deteksi Strain Pseudomonas solanacearum Penghasil Bakteriosin

https://doi.org/10.22146/jpti.12890

Triwidodo Arwiyanto(1*), Sudarmadi Sudarmadi(2), I. Hartana(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Asosiasi Penelitian Perkebunan Indonesia
(*) Corresponding Author

Abstract


The capability of Pseudornonas solanacearum to produce bacteriocin was investigated with the
use oj Casamino-acid Peptone Glucose Agar. All strains tested produce bacteriocm with
various spectra. Strain 6, 36 and ./9 inhibited almost all of the other strains. The avirulent
type of those three strains. i.e. 6-op. 36-op and -19-op, still produced bacteriocln on the same
medium. The.concentratlon of carbon source and acidity of the medium did not have any effect
on the bacteriocin production. The bacterioctn produced by strains 6-op, 36-op and 49-op
were inactivated at 70°C for 30 minutes.


Keywords


Pseudomonas solanacearum; bacteriocin; avirulent

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References

Arwiyanto, T., M. Goto and Y. Takikawa. 1993. Characterization of Bacteriocins Produced by Pseudomonas solanacearum, Ann. Phytopath. Soc. Japan. 59: 114-122.

Arwiyanto. T., Sudarmadi dan I. Hartana. 1995. Karakteristik Patogen Penyebab Penyakit Layu Bakteri pada Tembakau Deli, Kongres Nasional XIII dan Seminar Ilmiah Perhimpunan Fitopatologi Indonesia, Mataram.

Bolton Jr., H., Fredrickson, J.K. and L.F. Elliot. 1993. Microbial Ecology of Rhizosphere. p. 27-63. In: F. B. Metting, Jr. (ed.), Soil Microbial Ecology. An Application in Agricultural and Environmental Management, New York : Marcel Dekker, Inc.

Bradley, D.E. 1967. Ultrastructure of bacteriophage and bacteriocins. Bacterial. Rev. 31: 230-314

Campbell, R. and Echandi, E. 1979. Bacteriocin production in Erwinia carotovora. Phytopathoiogy 69: 1-A3 (Abstr.)

Chaterjee, A.K.; Willis, J.W.; Gutterson, N.; Mills, D.; Niepold, F.; Warren, G.; Comai, L. and Clement, Z. 1993. Bacterial Genetics. P. 495-532. In: Klement, Z.; Rudolph. K. And D.C. Sands ( eds.), Methods in Phytobacteriology, Budapest : Akademiai Kiado.

Cintas, L.M.; Rodriguez, J.M.; Fernandes, M.F.; Sletten, K.: Nes, I.F.; Hernandez, P.E. and H. Holo. 1995. Isolation and Characterization of Pediocin L50, a New Bacteriocin from Pediococcus acidilactica with a Broad Inhibitory Spectrum. Appl. Env. Mtcrobiol. 61: 2643-2648

Cuppels, D.A. 1976. Bacteriocin production by Pseudomonas solanacearum. P. 120. In: Sequeira, L. and A. Kelman (eds.), Proceedings of the First International Planning Conference and Workshop on the Ecology and Control of Bacterial Wilt Caused by Pseudomonas solanacearum. Raleigh, North Carolina.

Cuppels, D.A.; Hanson, R.S. and Kelman, A. 1978. Isolation and characterization of bacteriocin produced by Pseudomonas solanacearum. J. Gen. Microbiol. 109:295-303

Echandi, E. 1976. Bacteriocin production by Corynebacterium michiganense. Phytopathology 66:430-432

Echandi, E. and Moyer, J.W. 1979. Production, properties and morphology of bacteriocins from Erwinia chrysanthemi, Phytopathology 69: 1204-1207

Endo. Y.; Tsuyama, H. and Nakatani, F. 1975. Studies on Production of Antibacterial Agent by Erwinia carotovora and Its Properties. Ann. Phytopath. Soc. Japan 41: 40-48

He. L.Y., Fan, Y.L., Xie, D.X. and Fang, F. 1988. lntraspecific antagonism and bacteriocin production of Pseudomonas solanacearum. In Fifth Int. Cong. Plant Pathol. Abstract of Papers PII 1-24, Kyoto, Japan.

Holliday. P. 1992. A Dictionary of Plant Pathology. New York: Cambridge University Press. 369 pp.

Kato, T.; Matsuda. T.; Yoneyama, Y.; Kato, H. and Nakamura, R. 1993. Isolation of Enterococcus faecium with Antibacterial Activity and Characterization of Its bacteriocin. Biosci.Biotech.Biochem. 57:551-556

Kerr, A. 1980. Biological Control of Crown Gall Through Production of Agrocin 84. Plant Disease 64: 25-30

Konisky, J. 1978. The Bacteriocins. P. 71-136 In: Ornston, L.N. and J.R. Sokatch (eds.), The Bacteria: A Treatise on Structure and Function. Vol VI. Bacterial Diversity, New York: Academic Press.

Okabe, K. and Goto, M. 1963. Bacteriophages of plant pathogens . Ann.Rev.Phytopathol. 1:397-418

Rifai, M.A.; Wijaya, E.A. dan Ermitati. 1993. Kamus Biologi, Fitopatologi. Jakarta: Departemen Pendidikan dan Kebudayaan. 111 hal.

Schaad, N.W. 1988. Laboratory Guide for Identification of Plant Pathogenic bacteria. 2nd Ed. St. Paul, Minnesota: APS Press. 164 pp.

Sequeira, L. 1992. Bacterial Wilt. London: CAB. 245 pp.

Vidaver, A.K.: Mathys, M.L.; Thomas, M.E. and Schuster, M.L. 1972. Bactcriocins of the phytopathogens Pseudomonas syringae, P. glycinea and P. phaseolicola. Can.J.Microbiol. 18: 705-713 18:705-713



DOI: https://doi.org/10.22146/jpti.12890

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