* Corresponding Author
Analisis Runutan Nukleotida Gen Protein Selubung Dua Strain Peanut Stripe Virus
https://doi.org/10.22146/jpti.12406 Hasriadi Mat Akin(1*)
(1) Fakultas Pertanian Universitas Lampung
(*) Corresponding Author
Abstract
Nucleotide sequence analysis of coat protein gene from two biological strains of peanut stripe virus (PStV), severe blotch (PStV-Isb) and stripe (PStV-Is), has been done to study molecular variation of these strains. Comparison of predicted amino acid sequence of PStV coat protein with other members of bean common mosaic virus (BCMV) subgroup was also carried out to study the taxonomic: relationship between PStV and BCMV subgroup. The CP gene from these strains contained 861 nucleotides (nts) and encoded 287 amino acids. There were seven nucleotides of the CP gene and one nucleotide of NIb cistron that were different between PStV-Is and PStV-Isb. However, only one nucleotide at position 9309 of the CP gene caused differential amino acid residue at position 49 of the coat protein, methionine (M) for PStV-Isb and isoleucine (I) for PStV-Is. Comparison of the ammo acid sequences revealed a sequence similarity of 89,9-90,9% of PStV and other BCMV subgroup. These results provide evidence that PStV and other BCMV subgroup are in the same virus with different strain.
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Akin, H.M. & Sudarsono. 1997. Characterization of Peanut Stripe Virus (PStV) Isolated from Various Provinces in Indonesia. Indon. J. Trop. Agric. 8: 13-20.
Broglio, E.P. 1995. Mutation Analysis of Cauliflower Mosaic Virus Gene VI: Changes in Host Range, Symptoms, and Discovery of Transactivation-positive, Noninfectious Mutans. Mol. Plant-Microbe Interact. 8: 755-760.
Collmer, C.W., M.F. Marston, S.M. Albert, S. Bajai, H.A. Mavil!e, S.E. Ruuska, E.J. Vesely & M.M. Kyie. 1996. The Nucleotide Sequence of the Coat Protein Gene and 3' Untranslated Region of Azuki Bean Mosaic Potyvirus, a Member of the Bean Common Mosaic Virus Subgroup Mol. Plant-Microbre Interact. 9 (8): 758-761.
Derrick, P.M., S.A. Carter & R.S. Nelson. 1997. Mutation of the Tobacco Mosaic Tobamovirus 126- and 183-kDa Protein: Effects on Phloem-Dependent Virus Accumulation and Synthesis of Viral Proteins. Mol. Plant-Microbe Interact. 10 (5): 589-596.
Francki, R.I.B., C.M. Fauquet, D.L. Knudson & F. Brown. 1991. Classification and Nomenclature of Viruses. Arch. Virol. 2: 351-156.
Gibbs, M.J., J. Armstrong, G.F. Weiller & A.D. Gibbs. 1997. Virus Evolution, A Window on Future. In Tepfer and E. Balazs (Eds.) Virus-resistant Transgenic Plants: Potential Ecological Impact. Springer. Paris.
Gunashinghe, U.B., S. Flasinski, R.S. Nelson & B.G. Cassidy. 1994. Nucleotide Sequence and Genome Organization of Peanut Stripe Potyvirus. J. Gen. Viral. 75: 2519-2526.
Hu, J.S. 1995. Purification, Host Range, Serology, and Partial Sequencing of Dendrobium Mosaic Potyvirus, A New Members of the Bean Common Mosaic Virus Subgroup. Phytopathology 85 (5): 542-546.
Kanavagh, T., M. Goulden, S. Santa Cruz, Chapme, I. Barker & D. Baukcombe. 1992. Molecular Analysis of a Resistance Breaking Strain of Potato Virus X. Virology 189: 609-617.
Saleh, N., K.J. Middleton, Y. Baliadi, N.Hom & D.V.R. Reddy. 1989. Research on Peanut Stripe Virus in Indonesia, p. 9-10. In ICRISAT. Summary Proceeding of the second Coordinators Meeting on Peanut Stripe Virus. 1-4 Agustus 1989. India.
Shaw, J.G., A.G. Hunt, T.P. Pirone & R.E. Rhoads. 1990. Organization and Expression of Potyvirus Genes. In Pirone, T.P. and J.G. Shaw (eds.). Viral genes and Plant Pathogenesis. Springer-Verlag, New York. 215 p.
Shukla, D.D. & C.W. Ward. 1988. Amino Acid Sequence Homology of Coat Proteins as A Basis for Identification and Classification of the Potyvirus Group. J. Gen. Viral. 69: 2703-2710.
Thomson, J.D., D.G. Higgins & T.J. Gibson. 1994. Clustal W: Improving the Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Position-Specific Gap Penalties and weight Matrix Choice. Nuc. Acid Res. 22: 4673-4680.
Ward, C.W. & D.D. Shukla. 1990. Taxonomy of Potyvirus: Current Problems and Some Solutions. Intervirology 32: 269-296.
Weiland, J.J. & M.C. Edwards. 1996. A Single Nucleotide Substitution in αa Gene Confers Oat Pathogenicity to Barley Stripe Mosaic Virus Strain ND18. Mol. Plant-Microbe Interact. 9(1): 62-67.
DOI: https://doi.org/10.22146/jpti.12406
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