Bioactivity of A Bacillus Thuringiensis Cry1AC Toxin to Spodoptera litura

https://doi.org/10.22146/jpti.12214

Y. Andi Trisyono(1*), Serafina T.S. Rahayu(2), St. Margino(3)

(1) Jurusan Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Gadjah Mada
(2) Jurusan Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Gadjah Mada
(3) Jurusan Mikrobiologi Pertanian Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The transgenic cotton expressing Bacillus thuringiensis toxin Cry1Ac in Indonesia has been planted since 2000 for controlling Helicoverpa armigera. Spodoptera litura is another lepidopteran insect that also attacks cotton. The objective of this research was to determine the toxicity of Cry1Ac to S. litura. The acute toxicity was determined using neonates of S. litura exposed to artificial diets treated with series concentrations of Cry1Ac ranging from 0.14 to 625 mg/ml, and larval mortality was recorded at the seventh day after treatment. The chronic toxicity was determined by exposing neonate to artificial diets treated with the sublethal concentrations (LC5 and LC40). The growth and development of treated larvae were compared with those of the control farvae. Cry1Ac was toxic to neonate with LC5 values of 71.9, 18.1, 24.7, and 16.2 mg/ml for S. litura collected from Wonosari, Bantul-l, Bantul-2, and Kopeng, respectively. Cry1Ac was more toxic than formulated B. thuringiensis (LC50 = 724.8 mg/ml). Larvae exposed continuously to artificial diets treated with sublethal concentrations of Cry1Ac (0.61 and 9.77 mg/ml) showed no significant difference on weight and length of each life stages than those of the control larvae. These indicate that the application of the tested sublethal concentrations of Cry I Ac did not affect the growth and development of S. litura. However, increasing concentrations of Cry1Ac (156.25 and 625 mg/ml) significantly reduced the weight of surviving larvae. These findings suggest that application of Cry1Ac to S. litura shows some toxicological effects. The effectiveness of the transgenic cotton in controlling S. litura in field situation will be discussed.

Keywords


Bacillus thuringiensis; Cry1Ac; Spodoptera litura

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References

Adamczyk, J. J., Jr. & D. V. Sumerford. 2001. Potential Factors Impacting Season-Long Expression of Cry1Ac in 13 Commercial Varieties of Bollgard Cotton. 06 pp. J. Insect Sci.1: 13. Available online: http://insectscience.org/1.13.

Adamczyk, J. J., Jr., V. J. Mascarenhas, G. E. Church, B. R. Leonard, & J.B. Graves. 1998. Susceptibility of Conventional and Transgenic Cotton Bolls Expressing the Bacillus thuringiensis Cry1A(c) a-Endotoxin to Fall Armyworm (Lepidoptera: Noctuidae) and Beet Armyworm (Lepidoptera: Noctuidae) injury. J. Agric. Entomol. 15: 163-171.

Aronson, A. I., W. Beckman, & P. Dunn. 1986. Bacillus thuringiensis and Related Insect Pathogens. Microbial. Rev. 50: 1-24.

Baum, J. A., T. B. Johnson, & B. C. Charlton. 1999. Bacillus thuringiensis: Natural and Recombinant Bioinsecticide Products, pp 189-210. In F. R. Hall and J. J. Menn (eds.), Methods in Biotechnology.5 : Biopesticide Use and Delivery. Humana Press. Totowa.

Burgerjon, A. & D. Martouret. 1971. Determmation and Significance of the Host Spectrum of Bacillus thuringiensis, pp. 305-325. In H. D. Burgess and N. W. Hussey(eds.), Microbial Control of Insects and Mites. Academic Press. New York

Cooksey, F. E. 1971. Crystal Toxin : Biochemistry and Mode of Action, pp 247-274. In H. D. Burgess and N. W. Hussey (eds.), Microbial Control of Insects and Mites Academic Press. New York.

Croft, B. A. 1990. Arthropod Biological Control Agents and Pesticides. Wiley, New York.

Delannay, X. 1991. Testing Transgenic Plants, pp 11-15. In S. Brotonegoro, J. Dharrna, L. Gunarto and M. K. Kardin (eds.), Workshop on Agricultural Biotechnology. Ministry of Agriculture-Republic oflndonesia. Jakarta.

Endah, T. A., S. Teguh & W. L. Bibiana. 1991. Toksisitas Isolat Bacillus thuringiensis Berliner dari Tanah terhadap Crocidolomia binotalis Zeller (Lepidoptera; Pyralidae) dan Spodoptera litura Fab. (Lepidoptera; Noctuidae), pp 242-249. Dalam Kumpulan Makalah IV Hortikultura, Palawija, dan Kesehatan. PEI. Yogyakarta.

Fischhoff, D. A. 1996. Insect-Resistant Crop Plants, pp 214-227. In G. J. Persley (ed.), Biotechnology and Integrated Pest Management. CAB International. Wallingford. UK.

Gould, F. 1999. Deploying Pesticidal Engineered Crops in Developing Countries, pp 264-293. In G. J. Parsley (ed.), Biotechnology and Integrated Pest Management. University Press Cambridge.

Greenplate, J. T. 1999. Quantification of Bacillus thuringiensis Insect Control Protein Cry1Ac Over Time in Bollgard Cotton Fmit and Terminals. J. Econ. Entomol. 92: 1377-1353.

Harnoto, Mujiono, & A. Naito. 1987. Pengaruh Insektisida pada Konsentrasi Sublethal terhadap Keperidian Spodoptera litura F, pp 361-3 64. Dalam Prosiding Kongres Entomologi II. PEI. Jakarta.

Luttrell, R. G., A. Ali, S. Y. Young,& K. Knighten. 1998. Relative Activity of Commercial Formulations of Bacillus thuringiensis Against Selected Noctuid Larvae (Lepidoptera: Noctuidae). J. Entomol. Sci. 33:365-377.

Macintosh, S. C., T. B. Stone, S. R. Sims, P. L. Hunst, J. T. Greenplate, P. G. Marrone, F. J. Perlak, D. A. Fischoff, & R. L. Fuchs. 1990. Specificity and Efficacy of Purified Bacillus thuringiensis Proteins against Agronomical Important Insects. J. Inver. Pathol. 56: 258-266.

Magallona, E. D., M. Soehardjan, & H. Lumbatobing. 1990. Pesticides in Estate Crop Protection in Indonesia. Dirjen Perkebunan. Jakarta, 220 p.

Sims, S. R. 1995. Bacillus thuringiensis var. kurstaki [Cry1A(c)] Protein Expressed in Transgenic Cotton: Effects on Beneficial and Other Non-Target Insects. Southwest. Entomol. 20: 493-500.

Situmorang, J., S. Margino, L. Sembiring, & S. Sumarni. 1997. Sidik Cepat Isolat-Isolat Bakteri Patogenik terhadap Spodoptera litura F, Helicoverpa armigera Hub. dan Plutella xylostella L. Berkala Biologi 2: 101-114.

Trisyono, A. & M. E. Whalon. I 997. Fitness Costs of Resistance to Bacillus thuringiensis in Colorado Potato Beetle (Coleoptera: Chrysomelidae). J. Econ. Entomol. 90: 261-271.

Trisyono, Y. A., S. Sudjono, B. Triman, E. Mahrub, & Suputa. 2003. Susceptibility of Helicoverpa armigera in South Sulawesi Indonesia to a Bacillus thuringiensis Toxin Cry1Ac. J. Insect Sci. (In Press).

Whalon, M. E. & D. L. Norris. 1999. Field Management: Delivery of New Technologies to Growers, pp 595-608. In G. J. Parsley (ed.), Biotechnology and Integrated Pest Management. University Press Cambridge.



DOI: https://doi.org/10.22146/jpti.12214

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