Pembuatan Antiserum dan Kajian Serologi Virus Penyebab Penyakit Daun Keriting Kuning Cabai

https://doi.org/10.22146/jpti.12213

Sri Sulandari(1*), Rusmilah Suseno(2), Sri Hendrastuti Hidayat(3), Soemartono Sosromarsono(4), Jumanto Harjosudarmo(5)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Institut Pertanian Bogor
(3) Fakultas Pertanian Institut Pertanian Bogor
(4) Fakultas Pertanian Institut Pertanian Bogor
(5) Fakultas Pertanian Institut Pertanian Bogor
(*) Corresponding Author

Abstract


Virus identification based on the serological assay has been widely applied as a tool for plant virus detection. The aims of this research is to produce antiserum of the Pepper yellow leaf curl virus by rabbit immunization using purified gcminivirus of Segunung isolate. Identification of the virus was done by using modified I-ELISA and DIBA methods and also by using western blott. I-ELISA and DIBA methods were able to detect the geminivirus in the infected samples. The reactivity of antiserum was found to be similar amontI pepper isolates from different location (Segunung, Yogyakarta, Cugenang, and Lembang) ana those from different hosts (pepper, tobacco, tomato and Ageratum conyzoides) The antiserum could also be used for detection and identification of the Pepper yellow leaf curl virus in its vector. A single insect vector is sufficient for the detection of virus properly. The detection of geminivirus in its vector is very useful because it can be used to study the epidemic of the disease in the field. The I-ELISA and DIBA methods are very useful as tools for detecting the geminivirus. The methods are very easy to be carried out, fastly, and need only a minimum cost on operation. Geminivirus could also be identified by western blott analysis.

Keywords


antiserum; detection; Pepper yellow leaf curl virus; serology

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References

Ball, E. M., R.O. Hampton, S.H. de Boer, & N.W. Schaad. 1990. Polyclonal antibodies. p. 33 - 54. In R.O. Hampton, E,M. Ball,& S.H. De Boer (eds.), Serological methods. APS Press, Minnesota.

Banttari, E. E. & P. H. Goodwin. 1985. Detection of Potato virus S, X and Y by enzyme-linked immuno-sorbent assay on nitrocellulose membranes (Dot ELISA). Ann. Phytopath. Soc. Japan. 69: 202-205.

Brown, J.K. 1997. The biology and molecular epidemiology of geminivirus subgroup III. P.125 - 195. Di dalam: Stacey G., N.T. Keen (Eds.), Plant Microbe Interactions. Vol.2. Int. Thomson Publ.

Brown, J. F. & H. J Ogle. 1997. Plant Pathogens and Plant Diseases. Rockvale Publ. Australia. 556 p.

Clarck, M. F. &. A.N. Adams. 1977. Characteristics of microplate methods of enzyme-linked immunosorbent assay for detection of plant viruses. J. gen. Viral. 34: 475 - 483.

Harlow, E. & D. Lane. 1999. Using antibodies. A laboratory manual. Cold Spring Harbor Lab., New York. 358 p.

Kato, K., M. Onuki, S. Fuji, & K. Hanada. 1998. The first occurrence of Tomato yellow leaf curl virus in tomato (Lycopersicon esculentum Mill.) in Japan. Ann. Phytopath. Soc. Japan 64: 552 - 559.

Koenig R. 1981. Indirect ELISA methods for the broad specificity detection of plant viruses. J. gen. Virol 55.53-62.

Matthews, R. E. F. 1992. Foundamentals of plant virology. Acad. Press, Inc., California. 403 p.

McGrath, P. F. & B.D. Harrison. 1995. Transmission of Tomato leaf curl geminiviruses by Bemisia tabaci: effects of virus isolate and vector biotype. Ann. Appl. Biol. 1995 (126): 307-316.

Morales, F., A. Niessen, B. Ramirez, & M. Castano. 1990. Isolation and partial characterization of a geminivirus causing Bean dwarf mosaic. Phytopath. 80: 96 - 101.

Onuki, M., Y. honda, & K. Hanada. 2000. Germinate particle morphology of Sweet Potato leaf curlvirus in partially purified preparation and its serological relationship to two Begomoviruses by western blotting. J. gen. Plant Path. 66: 182-184.

Rojas, M.R., R.L. Gilbertson, D.R. Russell, & D.P. Maxwell. 1997. Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminivirus. Plant Dis. 77: 340- 347.

Torrance L. 1992. Serological methods to detect plant viruses: Production and use of monoclonal antibodies. p. 7 - 45. In J.M. Duncan, L. Torrance. editor. Technique for the rapid detection of plant pathogens. Blackwell Scientific Publications.

Trisusilowati, E. B. 1989. Studi sifat virus penyebab penyakit kerupuk pada tanaman tembakau (Nicotianatabacum L.). Disertasi S3. Program Pasca Sarjana lnstitut Pertanian Bogor.

Van Regenmortel, M. V. H. 1982. Serology and immunochemistry of plant viruses. Acad. Press, New York. 302 p.

Van Regenmortel M.H.V., C.M. Fauquet, D.H.L. Bishop, E.B. Carsthis, M.K. Estes, S.M. Lemon, J. Maniloff, M.A. Mayo, D.J. Mc. Geoch, C.R. Pringle, & R.B. Wick:ner. 2000. Virus Taxonomy, Classification and Nomenclature of Viruses. Seventh report of the ICTV. Acad. Press. 1162 p.

Wyatt, S. D. & J.K. Brown. 1996. Detection of subgroup III geminiviruses isolates in leaf extract by degenerate primers and polymerase chain reaction. Phytopath. 86: 1288- 1293.



DOI: https://doi.org/10.22146/jpti.12213

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