Tipe Mating pada Empat Isolat Thanatephorus Cucumeris Anamorf: Rhizoctonia solani) Anastomosis Group (AG) 1-IC


Achmadi Priyatmojo(1*)

(1) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author


Four parent isolates (189, Rh28, BW3 and F-1) of Thanatephorus cucumeris (anamorph: Rhizoctonia solani) AG 1-IC were induced to produce basidiospores using soil-over culture method. All of four parent isolates could produce basidiospores. Colonies obtained from single basidiospore isolate of each parent isolate were paired on charcoal potato dextrose agar. Single basidiospore isolate having different mating type produced tuft at area of the junction of paired colonies. On the based of tuft formation, single basidiospore isolates of each parent isolate could be divided into two different mating types, therefore it is concluded that each of 189, Rh28, BW3 and F-1 isolate of T. cucumeris AG I-IC has bipolar mating type.


basidiospore; bipolar; induction of teleomorph; mating type; Rhizoctonia solani; Thanatephorus cucumeris

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Adams, G. C. 1988. Thanatephorus cucumeris (Rhizoctonia solani) a species complex of wide host range, p. 535-552. In G. S. Sidhu (ed), Advances in Plant Pathology. Vol 6; Genetics of Plant Pathogenic Fungi. Academic Press, New York.

Adams, G. C. & E.E. Butler. 1983. Influences of nutrition on the formation of basidia and basidiospores in Thanatephorus cucumeris. Phytopathology 73: 14 7-151.

Anderson, N. A. 1982. The genetics and pathology of Rhizoctonia solani. Annual Review of Phytopathology 20: 329-344.

Aoyagi, T., Kageyama, K. & M. Hyakumachi. 1998. Characterization and survival of Rhizoctonia solani AG 2-2 LP associated with large patch disease of zoysia grass. Plant Disease 82: 857-863.

Bandy, B.P., Leach, S.S. & S.M. Tavantzis. 1988. Anastomosis group 3 is the main cause of Rhizoctonia disease of potato in Maine. Plant Disease 72: 596-598.

Benson, D.M. & D.K. Cartwright. 1996. Ornamental diseases incited by Rhizoctonia spp., p. 303-314. In B. Sneh, S. Jabaji-Hare, S. Neate, and G. Dijst, (eds.), Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control. Kluwer Academic Publishers, Dordrecht, the Netherlands

Burpee, L.L. & B. Martin. 1992. Biology of Rhizoctonia species associated with turfgrasses. Plant Disease 76: 112-117.

Carling, D. E. 1996. Grouping in Rhizoctonia solani by hyphal anastomosis reaction, p. 37-47. In B. Sneh, S. Jabaji-Hare, S. Neate & G. Dijst (eds), Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology, and Disease Control. Kluwer Academic Publishers, Dordrecht.

Carling, D. E., Baird, R. E., Gitaitis, R. D., Brainard, K.H. & S. Kuninaga. 2002. Characterization of AG-13, a newly reported anastomosis group of Rhizoctonia solani. Phytopathology 92: 893-899.

Carling, D.E. & .S. Kuninaga. 1990. DNA base sequence homology in Rhizoctonia solani Kuhn: inter- and intragroup relatedness of anastomosis group 9. Phytopathology 80:1362-1364.

Hietala, A. M., Sen, R., & A. Lilja. 1994. Anamorphic and teleomorphic characteristics of a uninucleate Rhizoctonia sp. isolated from roots of nursery grown conifer seedlings. Mycological Research 98: 1044-1050.

Hyakumachi, M., Mushika, T., Ogiso, Y., Toda, T., Kageyama, K. & T. Tsuge. 1998. Characterization of a new cultural type (LP) of Rhizoctonia solani AG 2-2 isolated from warm-season turfgrasses, and its genetic differentiation from other cultural types. Plant Pathology 47: 1-9.

Hyakumachi, M. & A. Sumino. 1984. New morphological type (IC) in Rhizoctonia solani AG 1 isolated from the sugar beet-manufactory-waste- soils and some of its characteristics. Annals of the Phytopathological Society of Japan 50: 507-514.

Julian, M.C. Debets, F. & J. Keijer. 1996. Independence of sexual and vegetative incompatibility mechanisms of Thanatephorus cucumeris (Rhizoctonia solani) anastomosis group 1. Phytopathology 86: 566-574.

Julian, M.C.,Acero, J., Salazar, 0., Keijer, J. & V Rubio. 1999. Mating type-correlated molecular markers and demonstration of heterokaryosis in the phytopathogenic fungus Thanatephorus cucumeris (Rhizoctonia solani) AG 1-IC by AFLP DNA fingerprinting analysis. Journal of Biotechnology 67: 49-56.

Kangatharalingam, N. & M.L. Carson. 1988. Technique to induce sporulation in Thanatephorus cucumeris. Plant Disease 72: 146-148.

Kuninaga, S. &R. Yokosawa. 1984. DNA base sequence homology in Rhizoctonia solani Kuhn. IV Genetic relatedness within AG 4. Annals of the Phytopathological Society of Japan 50: 322-330.

Kuninaga, S., Yokosawa, R. &A. Ogoshi. 1979. Some properties of anastomosis group 6 and BI in Rhizoctonia solani Kuhn. Annals of the Phytopathological Society of Japan 45: 207-214.

MacNish, G.C., Carling, D.E. & K.A. Brainard. 1993. Characterization of Rhizoctonia solani AG 8 from bare patches by pectic isozyme (zymogram) and anastomosis techniques. Phytopathology 83: 922-927.

Menzeis, J. D. 1970. Introduction: the first century of Rhizoctonia solani, p. 3-5. In J.R., Jr. Parmeter ( ed), Rhizoctonia solani: Biology and Pathology. University of California Press, Berkeley.

Naito, S. 1996. Basidiospore dispersal and survival, p. 197-206. In B. Sneh, S. Jabaji-Hare, S. Neate & G Dijst, (eds), Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology, and Disease Control. Kluwer Academic Publishers, Dordrecht.

Ogoshi, A. 1976. Studies on the grouping of Rhizoctonia solani Kuhn with hyphal anastomosis, and on the perfect stage of groups. Bulletin Seri C, No. 3. National Institute of Agricultural Sciences, Tokyo, Japan.

Ogoshi, A. 1987. Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kuhn. Annual Review of Phytopathology 25: 125-143.

Ogoshi, A. 1996. Introduction - the genus Rhizoctonia, p 1-9. In B. Sneh, S. Jabaji-Hare, S. Neate & G Dijst, (eds), Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology, and Disease Control. Kluwer Academic Publishers, Dordrecht.

Ogoshi, A. Oniki, M., Araki, T., & T. Ui. 1983. Anastomosis groups of binucleate Rhizoctonia in Japan and North America and their perfect states. Transaction of Mycological Society of Japan 24: 79-87.

Parmeter, J.R. Jr. & Whitney, H.S. 1970. Taxonomy and nomenclature at the imperfect state, p. 7-19. In J.R. Jr. Parmeter (ed), Rhizoctonia solani: Biology and Pathology. University of California Press, Berkeley.

Priyatmojo, A. 2006. Perbandingan empat metode induksi stadium sempurna pada Thanatephorus cucumeris (anamorf: Rhizoctonia solani). Prosiding Seminar Nasional Hasil Penelitian Pertanian. Fakultas Pertanian UGM. Yogyakarta.15 September 2006. Halaman: 1-6.

Priyatmojo, A., Escopalao, V.E., Tangonan, N.G, Pascual, C.B., Suga, H., Kageyama, K. & M. Hyakumachi. 2001. Characterization of a new subgroup of Rhizoctonia solani anastomosis group 1 (AG 1-ID ), causal agent of a necrotic leaf spot on coffee. Phytopathology 91: 1054-1061.

Sneh, B., Burpee, L. & A. Ogoshi. 1991. Identification of Rhizoctonia Species. The American Phytopathological Society Press, St. Paul, MN.

Stretton, H.M., McKenzie, A.R., Baker K.F. & N.T. Flentje. 1964. Formation of the basidial stage of some isolates of Rhizoctonia. Phytopathology 54: 1093-1095

Talbot, P.H.B. 1970. Taxonomy and nomenclature of the perfect state.p. 20-31. In J.R. Jr. Parmeter (ed.), Biology and Pathology of Rhizoctonia solani. University of California Press, Berkeley.

Toda, T. & M. Hyakumachi. 2006. Heterokaryon formation in Thanatephorus cucumeris anastomosis group 2-2 IV. Mycologia 98: 726-736.

Tu, C.C. & J.W. Kimbrough. 1975. A modified soil-over-culture method for inducing basidia in Thanatephorus cucumeris. Phytopathology 65: 730- 731.

DOI: https://doi.org/10.22146/jpti.12077

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