* Corresponding Author
Karakterisasi Parsial Streptomyces spp., Agens Pengendali Hayati Peyakit Lincat Tembakau
https://doi.org/10.22146/jpti.11854 Triwidodo Arwiyanto(1*), Ari Astuti(2), Y. M. S. Maryudani(3)
(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Local isolates of Streptomyces spp. were proven could suppress "lincat disease" of tobacco in the field. Six isolates were chosen for partial characterization of their bacteriological properties as based for the next experiments purposes. The results indicated that the isolates produce miselium with spore chains, gram positive, aerob, catalase and oxidase positive. The isolates also hydrolize starch, gelatine and esculine; produce lecithinase enzyme, reduce nitrate to nitrite, do not produce melanine pigment, did not produce hydrogen sulfide. The isolates were sensitive against streptomycine and rifampicin; able to use several carbon and nitrogen sources tested. Capable to grow on several medium pH, from 4,3 to 8,0. The isolates were able to grow from 5° C to 45° C; able to grow on medium containing 4% to 7% NaCl and ion the medium containing 0,1% of phenol. Plant pathogenicity test result showed negative responses which indicated that the used isolates were non plant pathogenic. The ability in suppressing lincat pathogen (Ralstonia solanacearum and Meloidogyne incognita) in vitro was vary between isolates.
Keywords
Full Text:
PDFReferences
Arwiyanto, T. 1997. Biological Control of Tobacco Bacterial Wilt: 1. Isolation of Antagonistic Bacteria Indonesian Journal of Plant Protection 3: 54-60.
Arwiyanto, T., M. Goto, and Y Takikawa. 1993. Charactherization of Bacteriocins Produced by Pseudomonas solanacearum. Annals Phytopathol. Soc. Japan 59: 114-122.
Arwiyanto, T., K. Haryono, A. Priyatmojo, T. Martoredjo, dan Dalrnadiyo, G 2007. Penekanan Penyakit Lincat Tembakau Temanggung dengan Streptomyces. Jurnal Perlindungan Tanaman Indonesia 13: 13-21.
Dalmadiyo, G 2004. Kajian Interaksi Infeksi Nematoda Puru Akar (Meloidogyne incognita) dengan Bakteri Ralstonia solanacearum pada Tembakau Temanggung. Fakultas Pertanian UGM. Disertasi
Dropkin, VH. 1996. Introduction to Plant Nematology (Pengantar Nematologi Tumbuhan, alih bahasa Supratoyo ). Edisi ke-3. Gadjah Mada University Press. Yogyakarta. 366 hal.
Gordon, R.E., W.C. Haynes, and C.H. Pang. 1973. The Genus Bacillus. Agriculture Research Service. United States Department of Agriculture. Washington.
Hayward. 1994. Systematic and Phylogeny of Pseudomonas solanacearum and Related Bacteria. In: Hayward, A.C. and GL. Hartman (Eds.). Bacterial Wilt. The Disease and The Causative Pseudomonas solanacearum. CAB International. Wallingford. 123-136
Klement, Z., K. Rudolph, and D.C. Sands. 1990. Methods in Phytobacteriology. Akademia Kiado. Budapest. Locci, R. 1991. Streptomycetes and Related Genera. In Ballows, A., H.G Truper, M. Dworkin, W. Horder, and K.H. Schleisfer (Eds.). The Procaryotes, A Handbook on the Biology of Bacteria. Ecophysiology, Isolation, Identification, Application. 2nd Edition. Springler-Verlag. New York. 2451-2492.
Luc, M., R.A Sikora, and J. Bridge. 1995. Plant Parasitic Nematodes in Subtropic and Tropic Agriculture (Nematoda ParasitikTumbuhan di Pertanian Subtropik dan Tropik, alih bahasa Supratoyo). Gadjah Mada University Press. Yogyakarta. 838 hal.
Nishiyama, K. and A. Ezuka. 1991. Manual of Identification of Plant Pathogenic Bacteria. Japan Plant Protection Association. Tokyo.
Pridham, T.G. and H.D. Tresner. Streptomycetaceae Waksman and Heinrici, 1943. In: Buchanan, RE. and N.E. Gibbon (Eds.). Bergey's Manual of Determinative Bacteriology. 8th Edition. Williams and Walkins Co. Baltimore. 747- 829.105
Schaad, N. W. 200 I. Initial Identificatio of Common Genera. In: Schaad, N.W., J.B. Jones, and W. Chun (Eds.) Laboratory Guide for Identification of Plant Pathogenis Bacteria. 3rd Edition. APS Press. St Paul. Minnesota. 1-16.
Williams, T.S., GP. Sharples, and R.M. Bardshaw. 1973. The Fine Structure of the Actinomycetales. In: Sykes, G and F.A. Skinner (Eds.). Actinomycetales:Characteristics and Practical Importance. Academic Press. London. 113-127.
Liu D,Anderson N.A, Kinkel L.L.1995. Biological control of potato scab in the field with Streptomyces spp. Phytopathology 85:827-831.
DOI: https://doi.org/10.22146/jpti.11854
Article Metrics
Abstract views : 1875
| 
views : 2398
Refbacks
- There are currently no refbacks.
Copyright (c) 2007 Jurnal Perlindungan Tanaman Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perlindungan Tanaman Indonesia ISSN 1410-1637 (print), ISSN 2548-4788 (online) is published by the Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, in collaboration with Indonesian Entomological Society (Perhimpunan Entomologi Indonesia, PEI) and Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia, PFI). The content of this website is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
View website statistics