Karakterisasi Parsial Streptomyces spp., Agens Pengendali Hayati Peyakit Lincat Tembakau

https://doi.org/10.22146/jpti.11854

Triwidodo Arwiyanto(1*), Ari Astuti(2), Y. M. S. Maryudani(3)

(1) Fakultas Pertanian Universitas Gadjah Mada
(2) Fakultas Pertanian Universitas Gadjah Mada
(3) Fakultas Pertanian Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Local isolates of Streptomyces spp. were proven could suppress "lincat disease" of tobacco in the field. Six isolates were chosen for partial characterization of their bacteriological properties as based for the next experiments purposes. The results indicated that the isolates produce miselium with spore chains, gram positive, aerob, catalase and oxidase positive. The isolates also hydrolize starch, gelatine and esculine; produce lecithinase enzyme, reduce nitrate to nitrite, do not produce melanine pigment, did not produce hydrogen sulfide. The isolates were sensitive against streptomycine and rifampicin; able to use several carbon and nitrogen sources tested. Capable to grow on several medium pH, from 4,3 to 8,0. The isolates were able to grow from 5° C to 45° C; able to grow on medium containing 4% to 7% NaCl and ion the medium containing 0,1% of phenol. Plant pathogenicity test result showed negative responses which indicated that the used isolates were non plant pathogenic. The ability in suppressing lincat pathogen (Ralstonia solanacearum and Meloidogyne incognita) in vitro was vary between isolates.

 

Streptomyces spp, isolat lokal terbukti dapat menekan penyakit lunvat tembakau di lapangan. Sebanyak enam isolat dipilih untuk dicirikan sebagai sifat-sifat bakteriologinya sehingga dapat digunakan sebagai dasar dalam penelitian berikutnya. Penelitian dilakukan terhadap sifat morfologi, fisiologi dan sifat biokimia. Hasil penelitian menunjukkan bahwa isolat yang diteliti menghasilkan miselium yang memproduksi rangkaian spora, Gram positif, aerob, katalase dan oksidase positif. Isolat-isolat tersebut menghidrolisis pati, gelatin, eskulin; membentuk ensim lechitinase, mereduksi nitrat menjadi nitrit, tidak menghasilkan pigmen melanin, tidak membentuk hidrogen sulfida. Isolat yang diteliti peka terhadap antibiotik streptomisin dan nifampisin; mampu menggunakan beberapa sumber karbon dan sumber nitrogen yang diujikan, Kisaran pH untuk pertumbuhan sangat lebar mulai dari pH 4,3 sampai pH 8,0 sedangkan kisaran suhu pertumbuhannya dari 5C sampai 45C. Streptomyces spp yang diuji mampu tumbuh pada media yang mengandung NaCl 4% dan 7% serta dapat tumbuh pada medium yang mengandung 0,1% fenol. Hasil pengujian patogenisitas terhadap tanaman menunjukkan bahwa keenam isolat tersebut bukan patogen tumbuhan. Terdapat variasi di anatara enam isolat dalam kemmapuan menekan pertumbuhan patogen lincat secara in vitro, terhadap Ralstonia solanacearum maupun terhadap Meloidogyne incognita.


Keywords


Streptomycetes spp, agens pengendalian hayati, penyakit lincat tembakau; biologycal control agents, lincat disaese of tobacco

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DOI: https://doi.org/10.22146/jpti.11854

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