In Vivo Study of Telang Cream on Interleukin (IL)-6, Epidermal Growth Factor (EGF), and Wound Reepithelialization

Nike Pratiwi; Nurmayani Irwandi; Restu Syamsul Hadi; Nabila Pyrenina Thamrin; Ndaru Andri Damayanti; Juniarti Juniarti; Nunung Ainur Rahmah

doi:10.22146/jkesvo.106084

In Vivo Study of Telang Cream on Interleukin (IL)-6, Epidermal Growth Factor (EGF), and Wound Reepithelialization

https://doi.org/10.22146/jkesvo.106084

Nike Pratiwi^(1*), Nurmayani Irwandi⁽²⁾, Restu Syamsul Hadi⁽³⁾, Nabila Pyrenina Thamrin⁽⁴⁾, Ndaru Andri Damayanti⁽⁵⁾, Juniarti Juniarti⁽⁶⁾, Nunung Ainur Rahmah⁽⁷⁾

(1) Graduate Student on Biomedical Sciences, YARSI University
(2) Graduate Student on Biomedical Sciences, YARSI University
(3) Department of Biomedical Science, Post Graduate School, YARSI University
(4) Department of Biomedical Science, Post Graduate School, YARSI University
(5) Department of Biomedical Science, Post Graduate School, YARSI University
(6) Department of Biomedical Science, Post Graduate School, YARSI University. Department of Herbal Laboratory Research Center, Biomedical Science YARSI University
(7) Department of Biomedical Science, Post Graduate School, YARSI University Department of Anatomy Histology Laboratory Research Center, Biomedical Science, Post Graduate School
(*) Corresponding Author

Abstract

Background: Povidone iodine is widely used in wound healing, but it can cause irritation and cellular toxicity. Clitoria ternatea L. has the potential to contain compounds such as rutin, quercetin 3-O-rhamnoside, ternatin, and anthocyanins as natural alternatives in wound healing. In this study, the extract used contains anthocyanins.

Objective: The study aimed to evaluate the effects of Clitoria ternatea L. extract cream on the expression of Interleukin-6, Epidermal Growth Factor, and re-epithelialization in Sprague Dawley rat incision wounds.

Methods: An in-vivo study with a post-test only control design was conducted on five treatment groups, including a control group without wounds (healthy), povidone iodine, and Clitoria ternatea L. extract cream (5%, 10%, 15%). IL-6 and EGF were analyzed using immunohistochemistry, while re-epithelialization was assessed using hematoxylin-eosin staining.

Results: Clitoria ternatea L. extract increased IL-6 and EGF expression, with the highest expression at a 10% concentration. Re-epithelialization was faster in the treatment groups compared to both the positive and negative controls.

Conclusion: The 10% Clitoria ternatea L. extract cream demonstrated efficacy comparable to povidone iodine in incision wound healing.

Keywords

Clitoria ternatea L; IL-6; EGF; Re-epitelisasi; Penyembuhan luka.

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DOI: https://doi.org/10.22146/jkesvo.106084