EFEKTIVITAS VAKSIN POLIVALEN UNTUK PENGENDALIAN VIBRIOSIS PADA KERAPU TIKUS (Cromileptes altivelis)

https://doi.org/10.22146/jfs.9056

Kamiso Handoyo Nitimulyo(1*), Alim Isnansetyo(2), Triyanto Triyanto(3), Muhammad Murdjani(4), Lili Sholichah(5)

(1) Jurusan Perikanan Fakultas Pertanian UGM
(2) Jurusan Perikanan Fakultas Pertanian UGM
(3) Jurusan Perikanan Fakultas Pertanian UGM
(4) Balai Besar Pengembangan Budidaya Air Payau
(5) Balai Besar Pengembangan Budidaya Air Payau
(*) Corresponding Author

Abstract


The objective of this research was to know the effectiveness of polyvalen  Vibrio vaccine to control vibriosis in humpback grouper (Cromileptes altivelis). The effectiveness of vaccine was evaluated by the survival rate (SR), relative percent survival (RPS), mean time to death (MTD) as well as growth rate of vaccinated fish. This research consisted of 4 treatments (control, injection, immersion, and oral vaccinations) in quadruplicates. Injection  vaccination was conducted by intraperitoneal injection of polyvalen vaccine at 107 cells/fish. Immersion vaccination was done by immersing the fishes at 107 cells/ml for 30 minutes. Oral administration of vaccine was also carried out  at 107 cells/fish. One week after the first vaccination, second vaccination (booster) was carried out at the same dosage and by the same administration. One week after the second vaccination, fishes were challenged with 3.16x104 cells/fish of Vibrio ordalii 3J by intraperitoneal injection, and reared for 20 days post infection. Results indicated that polyvalen Vibrio vaccine increased SR (P<0.01) up to 100%. Vaccination was also able to delay MTD of fishes. However, the vaccination was not influence the growth rate of fish.


Keywords


Cromileptes altivelis, polyvalen vaccine, vibriosis

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References

Anonim. 2004. Vibriosis in fish. www.hmsc.orst.edu/classes/MB492/VanguillarumKim/Vibriosis.htm. Diakses tanggal 18 Agustus 2004.

Austin, B. and D.A. Austin. 1987. Bacterial fish patogens: diseases in farmed and wild fish. John Wiley and Sons. Chichester. 364 p.

Bullock, G.L. 1971. Diseases of fishes. Book 2A: bacterial diseases of fishes. TFH Pub. Nepture: 42-50

Effendie, M.I. 1997. Biologi perikanan. Yayasan Pustaka Nusatama. Bogor.

Ellis, A.E. 1988. General principles of fish vaccination. In: Fish Vaccination. A.E. Ellis (Ed.). Academic Press. London: 1-19.

Gomez, K.A. and A.A. Gomez. 1995. Statistical procedures for agricultural research. (Prosedur statistik untuk penelitian pertanian, diterjemahkan oleh E. Samsudin dan J.S. Baharsah). Universitas Indonesia Press. Jakarta. 689 p.

Inglis, V., R.J. Robert, and N.R. Bromage 1993. Bacterial diseases of fishes. Blackwell Scientific. Pub. London. 283 p.

Kamiso, H.N. dan Triyanto. 1992. Vaksinasi monovalen dan polivalen vaksin untuk mengatasi serangan Aeromonas hydrophilla pada ikan lele (Clarias sp.). Jurnal Ilmu Pertanian. 4(8): 447-464.

Larkins, P.E. 1993. Shrimp diseases and shrimp farm management on the Malacca straits coast of North Sumatra Province. NESPP/ODA and Directorate General of Fisheries of Indonesia. 108 p.

Murdjani, M. 1997. Pembenihan ikan kerapu tikus (Cromileptes altivelis) dalam bak terkendali di Loka Budidaya Air Payau Situbondo. Ditjen Perikanan. Departemen Pertanian. 9 p.

Murdjani, M. 2002. Identifikasi dan patolo-gi bakteri Vibrio alginolyticus pada ikan kerapu tikus (Cromileptes altivelis). Disertasi Doktoral. Program Pascasarjana. Universitas Brawijaya. Malang.

Supriyadi, H. dan A. Rukyani. 1990. Imunoprofilaksis dengan cara vaksi-nasi pada usaha budidaya ikan. Seminar Nasional II Penyakit Ikan dan Udang. Balai Penelitian Perikanan Air Tawar. Pusat Penelitian dan Pengembangan Perikanan. Badan Penelitian dan Pengem-bangan Pertanian: 64-70.

Wu, J., H. Lin, L. Jan, Y. Hsu, and Chang. 1981. Biological control of fish bacterial phatogen Aeromonas hydrophila by bacteriophage ah I. Fish Patol. 15(3/4): 271-276.

Yushimitsu, T., H. Eda, and K. Hiramatsu. 1986. Groupers final report marine-culture research and development in Indonesia. ATA-192. JICA: 103-129.



DOI: https://doi.org/10.22146/jfs.9056

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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