* Corresponding Author
Peningkatan Ketahanan Benih Ikan Nila Salin (Oreochromis niloticus) terhadap Penyakit Streptococcosis Melalui Vaksinasi Induk
https://doi.org/10.22146/jfs.60280 Rahmi Rahmi(1*), Khairun Nisaa(2), Akmal Akmal(3), Mardiana Mardiana(4), Andi Chadijah(5), Nur Insana Salam(6)
(1) Program Studi Akuakultur, Fackultas Pertanian, Universitas Muhammadiyah Makassar, Makassar, Sulawesi Selatan, Indonesia
(2) Program Studi Akuakultur, Universitas Cokroaminoto Makassar, Makassar, Selawesi Selatan, Indonesia
(3) Balai Perikanan Budidaya Air Payau Takalar, Takalar, Sulawesi Selatan, Indonesia
(4) Program Studi Akuakultur, Fakultas Pertanian, Universitas Bosowa Makassar, Makassar, Sulawesi Selatan, Indonesia
(5) Program Studi Akuakultur, Fackultas Pertanian, Universitas Muhammadiyah Makassar, Makassar, Sulawesi Selatan, Indonesia
(6) Program Studi Akuakultur, Fackultas Pertanian, Universitas Muhammadiyah Makassar, Makassar, Sulawesi Selatan, Indonesia
(*) Corresponding Author
Abstract
Penelitian bertujuan untuk menghasilkan benih ikan nila salin yang tahan terhadap penyakit streptococcocis melalui vaksinasi induk. Vaksin yang digunakan dalam penelitian ini adalah bakteri Streptococcus sp. yang dilemahkan dengan formalin 3% (v/v). Penelitian ini dilaksanakan pada Juni-September. Vaksin diinjeksikan pada indukan TKG 2 sebanyak 0,4 mL/kg (konsentrasi 1x109 CFU/mL). Penelitian terdiri dari tiga perlakuan yaitu indukan diinjeksi dengan phosphate buffered saline (PBS) (K), indukan diinjeksi vaksin satu kali (A1), dan indukan diinjeksi vaksin sebanyak dua kali dengan selang waktu dua minggu (A2). Benih yang dihasilkan dari masing-masing perlakuan, diuji tantang melalui perendaman dengan bakteri Streptococcus sp. 107 CFU/mL selama 30 menit pada 10, 15, dan 20 hari setelah penetasan. Parameter yang diamati adalah penetasan telur, aktivitas lisozim, kematian benih setelah uji tantang, dan persentase kelangsungan hidup relatif (RPS) benih. Hasil penelitian menunjukkan bahwa daya tetas telur pada perlakuan A2 (92,21%) secara nyata lebih tinggi (P<0,05) dibandingkan perlakuan induk lainnya, serta aktivitas lisozim (99,47-197,89 U/mL) sampai dengan hari ke-20. Angka kematian pada perlakuan A2 (2,34-45,21%) secara signifikan lebih rendah (P<0,05) dibandingkan perlakuan lain sampai hari ke-20. Nilai RPS indukan A2 tidak berbeda nyata (P>0,05) dengan perlakuan benih dari indukan A1 pada hari ke sepuluh tetapi lebih tinggi pada hari ke 20.
Keywords
Full Text:
PDFReferences
Abraham T.J., M.S. Namdeo, H. Adikesavalu & S. Banerjee. 2019. Pathogenicity and pathology of Streptococcus agalactiae in challenged mozambique tilapia Oreochromis mossambicus (PETERS 1852) Juveniles.
Bowden, T.J., P. Cook & J.H.W.M. Rombout. 2005. Development and function of the thymus in teleosts. Fish & Shellfish Immunology. 19: 413-427.
Ellis, A.E., 1988. Fish Vaccination. Academic Press. New York. 255p.
Evans, J.J., P.H. Klesius, C.A. Shoemaker & S. Al-Ahlani. 2006. First report of Streptococcus agalactiae and Lactococcus garviae from a wild bottlenose dolphin (Tursiop struncatus). Journal of Aquatic Animal Health. 18: 212-216.
Gudding, R., B. Willem & V. Muiswinkel. 2013. A history of fish vaccination Science-based disease prevention in aquaculture. Fish & Shellfish Immunology. 30: 1-6. doi.org/10.1016/j.fsi.2013.09.031.
Gudding, R. 2014. Vaccination as a Preventive Measure. In Gudding R, Lillehaug A, Øystein Evensen, Editors. Fish Vaccination, First Edition. Wiley and Sons, Ltd. Published. West Sussex. United Kingdom. 12-21
Hanif, A., V. Bakopoulos & G.J. Dimitriadis. 2004. Maternal transfer of humoral specific and non-specific immune parameters to sea bream Sparus aurata larvae. Fish & Shellfish Immunology. 17: 411-435.
Hanif, A., V. Bakopoulos & G.J. Dimitriadis. 2005. The effect of sea bream Sparus aurata broodstock and larval vaccination on the susceptibility by Photobacterium damsela subsp piscicida and on the humoral immune parameters. Fish & Shellfish Immunology. 19: 345-361.
Kahieshesfandiari, M., M.Y. Sabri, M.Y. Ina-salwany, M.D. Hassan, O. Noraini, A.A. Ajadi & A.I. Isiaku. 2019. Streptococcosis in Oreochromis sp.: is feed-based biofilm vaccine of Streptococcus agalactiae effective? Aquaculture International. 27:817–832. doi: 10.1007/s10499-019-00372-8
Magnadottir, B., S. Lange, S. Gudmundsdottir, J. Bogwald & R.A. Dalm. 2005. Ontogeny of humoral immune parameters in fish. Fish & Shellfish. Immunology. 19: 429-439.
Mingming, H., D. FuHong, M. Zhen & L. Jilin. 2014. The effect of vaccinating turbot broodstocks on the maternal immunity transfer to offspring immunity. Fish & Shellfish Immunology. 30: 1-7. doi: org/10.1016/j.fsi.2014.03.010.
Nisaa, K., J.M.Z. Sukenda, A.M. Lusiastuti & S. Nuryati. 2016. Benih keturunan induk ikan nila yang divaksinasi pada tingkat kematangan gonad-2 lebih tahan terhadap infeksi Streptococcus agalactiae. Jurnal Veteriner. 17 (3): 355-364.
Nisaa, K., J.M.Z. Sukenda, A.M. Lusiastuti & S. Nuryati. 2017. Fry tilapia oreochromis niloticus antibody improvement against Streptococcus agalactiae through broodstock vaccination. Pakistan Journal of Biotechnology. 14: 9-16
Rauta, P.R., N. Bismita & S. Das. 2012. Immune system and immune responses in fish and their role in comparative immunity study: A model for higher organisms. Immunology Letters. 148: 23-33.
Secombes, C.J. 2011. Fish immunity: the potential impact on vaccine development and performance. Aquaculture Research. 42: 90-92.
Schroeder, H.W & L. Cavacini. 2010. Structure and function of immunoglobulins. Journal of Allergy & Clinical Immunology. 125: 41-52.
Sukenda, S., T.R. Febriansyah & S. Nuryati. 2014. Efikasi vaksin sel utuh Streptococcus agalactiae pada ikan nila Oreochromis niloticus melalui perendaman. Jurnal Akuakultur Indonesia. 13: 83-93.
Sukenda, S., R. Rusli, S. Nuryati & D. Hidayatullah. 2015. Durasi proteksi vaksin Streptococcus agalactiae untuk pencegahan streptococcosis pada ikan nila. Jurnal Akuakultur Indonesia.
Sukenda, S., R. Rahman, K. Nisaa, D. Hidayatullah & V. Apriana. 2018. The efficacy of Streptococcus agalactiae vaccine preparations, administered to tilapia broodstock, in preventing streptococcosis in their offspring, via transfer of maternal immunity. Aquaculture Int. 26 : 785–798
Swain, P., S. Dash, J. Bal, P. Routray, P.K. Sahoo, S.K. Sahoo, S. Saurabh, S.D. Gupta & P.K. Meher. 2006. Passive transfer of maternal antibodies and their existence in eggs, larvae and fry of Indian major carp, Labeo rohita. Fish & Shellfish Immunology. 20: 519-527.
Swain,P & S.K. Nayak. 2009. Role of maternally derived immunity in fish. Fish and Shellfish immunolog 27: 89-99.
Taukhid, T., U. Purwaningsih & A.M. Lustiastuti. 2012. Pengambangan vaksin inaktiv bakteri Streptococcus agalactiae: Penentuan teknik aplikasi dan dosis efektif vaksin melalui perendaman untuk pencegahan penyakit streptococcosis pada ikan nila Oreochromis niloticus. Prosiding seminar. Bogor (ID). Balai Riset Perikanan Budidaya Air Tawar.
Wang, Z.P & S.C. Zhang. 2010. The role of lysozyme and complement in the antibacterial activity of zebrafish Danio rerio egg cytosol. Fish & Shellfish Immunology. 29: 773-777.
Wang, H., D. Ji, J. Shao & S. Zhang. 2012. Maternal transfer and protective role of antibodies in zebrafish Danio rerio. Molecular Immunology. 51: 332-336.
Wang, N., Z. Yang, M. Zang, Y. Liu & C. Lu. 2013. Identification of Omp38 by immunoproteomic analysis and evaluation as a potential vaccine antigen against Aeromonas hydrophila in Chinese breams. Fish & Shellfish Immunology. 34: 74-81.
Yi, T., W.Y. Li, L. Liu, X.X. Xiao & A.X. Li. 2014. Protection of nile tilapia Oreochromis niloticus L. against Streptococcus agalactiae following immunization with recombinant FbsA and α-enolase. Aquaculture. 428-429: 35-40.
Zhang, S., Z. Wang & H. Wang. 2013. Maternal immunity in fish: Review. Developmental & Comparative Immunology. 39: 72-78.
DOI: https://doi.org/10.22146/jfs.60280
Article Metrics
Abstract views : 5381
| 
views : 6548
Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Jurnal Perikanan Universitas Gadjah Mada
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
View My Stats