* Corresponding Author
The Discovery of Vibrio harveyi on Litopenaeus vannamei Infected White Feces Disease in Situbondo, East Java
https://doi.org/10.22146/jfs.47791 Sumini Sumini(1*), Rahayu Kusdarwati(2)
(1) Brackishwater Aquaculture Development Center Situbondo, East Java, Indonesia
(2) Department of Fish Health and Aquaculture Management, Faculty of Fisheries and Marine Science, Airlangga University, East Java, Indonesia
(*) Corresponding Author
Abstract
This research was conducted to discover Vibrio harveyi infected vannamei shrimp with White Feces Disease (WFD) in Situbondo, East Java Province. This research was conducted in November 2017 until September 2018. All Vibrio isolates from shrimp infected WFD were identified with biochemical tests, Analytical Profile Index/ API 20NE (BioMeriuex), and PCR with specific primers for V. harveyi. Additional parameters were the water quality, plankton brackishwater pond abundance, and antibiotic resistance test. Result showed that from 17 bacterial isolates identified, 10 isolates were V. harveyi (58.82%), three isolates were V. alginolyticus (17.65%), one V. fluvialis isolate (5.88%), one V. parahaemolyticus isolate (5.88%), and two non Vibrio isolates which were identified as Shewanella putrefaciens (11.76%). All isolates of V. harveyi also showed resistance activity on more than one antibiotic. Poor water quality had been identified as abnormal result of pH, alkalinity, salinity, ammonia levels and total organic matter level. Plankton abundance observation showed that Chloropyceae, Diatom, and Dinoflagellata dominated all sampled brackishwater ponds. This research concluded that V. harveyi were discovered on vannamei shrimp with poor water quality and plankton abundance in the pond samples.
Keywords
Full Text:
PDFReferences
Austin, B & D.A. Austin. 1999. Bacterial Fish Pathogens: Disease of Farmed and Wild Fish. 3rd edition (Revised). Springer-Praxis Series in Aquaculture and fisheries. 102-276
BioMeriuex. 2003. Manual Instruction of API20NE. http://www.biomerieux.com. 26/09/2017. 4 pp
Boyd, C.E. 2014. Phytoplankton a crucial component of aquaculture pond ecosystems. School of Fisheries. Aquaculture and Aquatic Sciences. Auburn University. 4 pp
Buller, N.B. 2004. Bacteria from fish and other aquatic animals: A practical identification manual. Biddles Ltd. King’s Lynn. London. 138-176 pp
California Department of Public Health. 2014. HABs and shellfish safety: Red tides and harmful algal blooms. California Department of Public Health. 2
Cao, H., L. Wen, S. He, L. Lu, X. Yang & B. Chen. 2015. Vibrio cholerae: A causal agent for white feces syndrome in freshwater cultured whiteleg shrimp (Penaeus vannamei). The Israeli Journal of Aquaculture. 67: 1-7
Case´, M., E.E. Leca, S.N. Leita˜o, E.E.S. Anna, R. Schwamborn & A.T.M. Junior. 2008. Plankton community as an indicator of water quality in tropical shrimp culture ponds. Marine Pollution Bulletin. 56: 1343-1352
Chythanya, R., I. Karunasagar & I. Karunasagar. 2002. Inhibition of shrimp pathogenic vibrios by a marine Pseudomonas I-2 strain. Aquaculture. 208: 1-10
Clinical and Laboratory Standards Institute/ CLSI. 2012. Performance standards for antimicrobial susceptibility testing; twenty-second informational supplement. Clinical and Laboratory Standards Institute. West Valley. 6
Espinosa, I., M. Báez, M. I. Percedo & S. Martínez. 2013. Evaluation of simplified DNA extraction methods for Streptococcus suis typing. Revista the Salud Animal. 35 (1): 59-63
Fukui, Y & T. Sawabe. 2007. Improved one-step colony PCR detection of Vibrio harveyi. Microben. Environment International. 22 (1): 1-10
Heenatigala, P.P.M & M. Fernando. 2016. Occurrence of bacteria species responsible for vibriosis in shrimp pond culture systems in sri lanka and assessment of the suitable control measures. Sri Lanka Journal Aquatic Science. 21 (1): 1-17
Holt, J.G, N.R. Krieg, P.H.A. Sneath, J.T. Staley & S.T. Williams. 1994. Bergey's manual of determinative bacteriology. Ninth Edition. Springer Science-Business Media. A Waverly Company. 190-269 pp
Hou, D., Z. Huang, S. Zeng, J. Liu, D. Wei, X. Deng, S. Weng, Q. Yan & J. He. 2018. Intestinal bacterial signatures of white feces syndrome in shrimp. Applied Genetics And Molecular Biotechnology. 9 pp
Inthusai, R. 2006. Study on causes of white feces syndrome in black tiger shrimp Penaeus monodon culure in low salinity water. Master of science (Fisheries Sciences) Department of Fishery Biology. Kasetsart University. 1-7 pp
Jayadi, M., A. Prajitno & Maftuch. 2016. The identification of Vibrio spp. bacteria from Litopenaeus vannamei infected by white feces syndrome. International Journal of ChemTech Research. 9 (7): 448-452
Kharisma, A & A. Manan. 2012. Kelimpahan bakteri Vibrio sp. pada air pembesaran udang vannamei (Litopenaeus vannamei) sebagai deteksi dini serangan penyakit vibriosis. Jurnal Ilmiah Perikanan dan Kelautan. 4 (2): 129-134
Limsuwan, C. 2010. White feces disease in thailand. Boletines Nicovita. 4 (4): 2-4
MacFaddin, J.F. 1980. Biochemical Test for Identification of Medical Bacteria. Second Edition. Williams & Wikins Baitimore/ London. 370-389 pp
Malaroli, R & A. Gopalakrishnan. 2016. Comparative study on water quality parameter of normal and white feces syndrome affected shrimp ponds. International Journal of Science Inventions Today. 5 (4): 510-516
Manjusha, S., G.B. Sarita, K.K. Elyas & M. Chandrasekaran. 2005. Multiple antibiotic resistances of Vibrio isolates from coastal and brackish water areas. American Journal of Biochemistry and Biotechnology. 1: 20-206
Mastan, S.A. 2015. Incidences of white feces syndrome (WFS) in farm-reared shrimp, Litopenaeus vannamei. Indo American Journal of Pharmaceutical Research. 3044-3047
Oakey, H.J., N. Levy, D.G. Bourne, B. Cullen & A. Thomas. 2003. The use of PCR to aid in the rapid identification of Vibrio harveyi isolates. Journal of Applied Microbiology. 95: 1293-1303.
Percival, S.L & D.W. Williams. 2014. Vibrio. Microbiology of Waterborne Diseases. 237-248
Pusat Karantina Ikan. 2013. Standar Operasional Prosedur Pengambilan Contoh Media Pembawa Hidup dan Non Hidup. Jakarta. 47 pp
Raja, R.A., R. Sridhar, C. Balachandran, A. Palanisammi, S. Ramesh & K. Nagarajan. 2017. Pathogenicity profile of Vibrio parahaemolyticus in farmed pacific white shrimp, Penaeus vannamei. Fish and Shellfish Immunology. 67: 368-381
Rajendran, K.V, S. Shivam, P.E. Praveena, J.J.S. Rajan, T.S. Kumar, S. Avunje, V. Jagadeesan, S.V Babu, A. Pande, A.N. Krishnan, S.V. Alavandi & K.K. Vijayan. 2016. Emergence of Enterocytozoon hepatopenaei (EHP) in farmed Penaeus (Litopenaeus vannamei) in India. Journal Aquaculture. 454: 272-280
Rodriguez, R.A & F.P. Osuna. 2003. Nutrients, phytoplankton and harmful algal blooms in shrimp ponds: a review with special reference to the situation in the Gulf of California. Aquaculture. 219: 317-336
Somboon, M., W. Purivirojkul, C. Limsuwan & N. Churchird. 2012. Effect of Vibrio spp. in white feces infected shrimp in chanthaburi, thailand. Kasetsart University Fisheries Research Bulletin. 36 (1): 7-15
Sriurairatana, S., V. Boonyawiwat, W. Gangnonngiw, C. Laosutthipong, J. Hiranchan & T.W. Flegel. 2014. White feces syndrome of shrimp arises from transformation, sloughing and aggregation of hepatopancreatic microvilli into vermiform bodies superficially resembling gregarines. PLoS ONE. 9 (6): 1-8
Standar Nasional Indonesia/ SNI. 2006. SNI 01-2332.4-2006. Cara uji mikrobiologi – bagian 4: Penentuan Vibrio cholerae pada produk perikanan. 10
Standar Nasional Indonesia/ SNI. 2014. SNI 8008:2014. Produksi udang vaname (Litopenaeus vannamei, Boone, 1931) intensif di tambak lining. Badan Standarisasi Nasional. Jakarta. 15 pp
Tang, K.F.J., J.E. Han, L.F. Aranguren, B. White-Noble, M.M. Schmidt, P. Piamsomboon, E. Risdiana & B. Hanggono. 2016. Dense populations of the microsporidian Enterocytozoon hepatopenaei in feces of Penaeus vannamei exhibiting WFS and pathways of their transmission to healthy shrimp. Journal of Invertebrate Pathology. 140: 1-7
Thompson, C.C.F., L. Thompson, A.C.P. Vicente & J. Swings. 2007. Phylogenetic analysis of vibrios and related species by means of atpA gene sequences. International Journal of Systematic and Evolutionary Microbiology. 57 : 2480-2484
DOI: https://doi.org/10.22146/jfs.47791
Article Metrics
Abstract views : 4626
| 
views : 4523
Refbacks
- There are currently no refbacks.
Copyright (c) 2020 Jurnal Perikanan Universitas Gadjah Mada
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
View My Stats