* Corresponding Author
Susceptibility of Tiger Shrimp (Panaeus monodon) against Vibrio harveyi on Various Molt Stage and Osmolarity
https://doi.org/10.22146/jfs.10327 Gina Saptiani(1*), Catur A Pebrianto(2), Agustina Agustina(3), Esti H Hardi(4)
(1) Laboratory of Aquatic Microbiology. Faculty of Fisheries and Marine Science. Mulawarman University
(2) Laboratory of Aquatic Microbiology. Faculty of Fisheries and Marine Science. Mulawarman University
(3) Laboratory of Aquatic Microbiology. Faculty of Fisheries and Marine Science. Mulawarman University
(4) Laboratory of Aquatic Microbiology. Faculty of Fisheries and Marine Science. Mulawarman University
(*) Corresponding Author
Abstract
Vibrio harveyi causes disease of tiger shrimp (Penaeus monodon) aquaculture in East Kalimantan. This research aimed to investigate the susceptibility of tiger shrimp on various molt stage and osmolarity, and determine the salinity (osmolarity) of water that can reduce the risk of V. harveyi attack. Shrimp age of 1.5 months was maintained in 4 aquariums with salinity 29.72 ‰, 26.07 ‰, 22.35 ‰ and 17.79 ‰, then challenged with V. harveyi. After 7 and 14 days, osmolarity of haemolimph was observed with automatic osmometer and Digital Roebling Osmometer. The susceptibility of tiger shrimp to V. harveyi was evaluated based on clinical symptoms, pathological anatomy of shrimp and density of V. harveyi (TPC) in the hepatopancreas. Osmolarities of shrimp haemolymph at premolt, postmolt, and intermolt stage were 575.30-812.60; 534.00-788.80, and 566.20-795.60 mOsm/l H2O, respectively. The lowest bacterial content in the premolt stage was maintained at a salinity 22.35 ‰, and the highest one was obtained in postmolt stage at 29.72 ‰. The molting and postmolt stage were the most susceptible to V. harveyi attacks. The results suggested that the shrimp maintained at 22.35 ‰ salinity was less susceptible to V. harveyi.
Keywords
Full Text:
PDFReferences
Anggoro, S. 1992. Efek Osmotik Berbagai Tingkat Salinitas Media terhadap Daya Tetas Telur dan Vitalitas Larva Udang Windu Penaeus monodon Fabricus. Disertasi. Program Pascasarjana IPB. Bogor. 362 hal.
Anggoro S., Muryati, Subandiyono & T. Supratno. 2007. Tingkat kerja osmotik dan efisiensi pemanfaatan pakan juvenil udang jahe (Metapenaeus elegans) asal segara anakan yang didomestikasi pada berbagai tingkat salinitas media. Bull. Penelitian dan Pengembangan Industri. 2(1): 1-6.
Anggoro, S. & Subandiyono. 2012. Osmotic Responses of Segara Anakan Fine Shrimp (Metapenaeus elegans) Adults in Various Salinity and Moulting Stages. Journal of Coastal Develpopment 15(3): 310-314.
Bacheri, D., G.H. Rafiee, B.M. Amiri, A. Mirvaghefia & D.A. Ali. 2010. The effects of salinity on the haemolymph osmolality and ionic level in white shrimp (Litopenaeus vannamei). J. Fisheries (Iranian J. Natural Resources). 63(3): 161-171.
Bonilla-Gómez, J.L., X. Chiappa-Carrara, C. Galindo, G. Jeronimo, G. Cuzonand & G. Gaxiola. 2012. Physiological and biochemical changes of wild and cultivated juvenile. J Crustacean Biology 32(4): 597-606.
Gao Y., X. Zhang, J. Wei, X. Sun, J. Yuan, F. Li & J. Xiang. 2015. Whole transcriptome analysis provides insights into molecular mechanisms for molting in Litopenaeus vannamei. Plos one 10(12): e0144350.
Heenatigala, P.P.M. & M.U.L. Fernando. 2016. Occurrence of bacteria species responsible for vibriosis in shrimp pond culture systems in Sri Lanka and assessment of the suitable control measures. Sri Lanka J. Aquat. Sci. 21 (1): 1-17
Jia, X., F. Wang, Y. Lu, D. Zhang & S. Dong. 2014. Immune responses of Litopenaeus vannamei to thermal stress: a comparative study of shrimp in freshwater and seawater conditions. J. Marine and Freshwater Behaviour and Physiology 47(Issue 2). http://dx.doi.org/10.1080/10236244.2014.894349
Kiruthika, J., S. Rajesh, K.V. Kumar, G. Gopikrishna, H. Imran Khan, E.P. Madhubabu, M. Natarajan, S. Dayal, A.G. Ponniah & M.S. Shekhar. 2013. Effect of Salinity Stress on the Biochemical and Nutritional Parameters of Tiger Shrimp Penaeus monodon. Fishery Technology. 50 (2013) : 294 - 300.
Maicá, P.F., M.R. de Borba, T.G. Martins & W.W. Junior. 2014. Effect of salinity on performance and body composition of Pacific white shrimp juveniles reared in a super-intensive system. R. Bras. Zootec. 43(7). http://dx.doi.org/10.1590/S1516-35982014000700001
Saptiani, G., S.B. Prayitno & S. Anggoro. 2012. The effectiveness of Acanthus ilicifolius in protecting tiger prawn (Penaeus monodon F.) from Vibrio harveyi infection. J. of Coast. Dev. 15(2):217-224.
Saptiani, G & C.A. Pebrianto. 2013. Total haemosit udang windu (Penaeus monodon) pada berbagai stadia molt dan osmolaritas. Prosiding Seminar Nasional Tahunan X Hasil Penelitian Perikanan dan Kelautan Jilid II 31 Agustus 2013. Yogyakarta. Semnaskan_UGM/Biologi Perikanan (BP-21): 1-6.
Selvam, D.G., K.M.M. Rahiman & A.A.M.Hatha. 2012. An Investigation into Occasional White Spot Syndrome Virus Outbreak in Traditional Paddy Cum Prawn Fields in India. Scientific World Journal. 2012: 340830. Published online 2012 Apr 19.
Vaseeharan, B., P. Ramasamy, S.G. Wesley & J.C. Chen. 2013. Influence of acute salinity changes on biochemical, hematological and immune characteristics of Fenneropenaeus indicus during white spot syndrome virus challenge. Microbiol Immunol. 57: 463–469.
Wade N.M., M. Anderson, M.J. Sellars, R.K. Tume, N.P. Preston & B.D. Glencross. 2012. Mechanisms of colour adaptation in the prawn Penaeus monodon The journal of Experimental Biology 215:343-350
DOI: https://doi.org/10.22146/jfs.10327
Article Metrics
Abstract views : 2526
| 
views : 4863
Refbacks
- There are currently no refbacks.
Copyright (c) 2016 Journal of Fisheries Sciences
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
View My Stats