Subchronic Toxicity Test of Kebar Grass Extract of n-Hexane (Biophytum Petersianum Klotzsch) on Weight, ADG and Vital Organ Weight of Rat (Rattus Norvegicus)

Priyo Sambodo(1*), Dwi Nurhayati(2), Purwaningsih Purwaningsih(3), Trini Susmiati(4), Claude Mona Airin3(5)

(1) Faculty of Animal Husbandry, Papua University
(2) Faculty of Animal Husbandry, Papua University
(3) Faculty of Animal Husbandry, Papua University
(4) Department of Biochemestry, Faculty of Veterinary Medicine, Gadjah Mada University of Yogyakarta
(5) Department of Physiology, Faculty of Veterinary Medicine, Gadjah Mada University of Yogyakarta
(*) Corresponding Author


The recent study was carried out to determine the effect of Kebar grass extract of N-hexane  (Biophytum petersianum Klotzsch) for 28 days peroral on weight, ADG and weight of vital organs of white rat. Forty male and female rats of Sprague Dawley strain aged 8 weeks were examined. The formulated rats were divided into four groups, group I (control), group II, III, IV (treatment), in which each consisted of five rats. The feed were given as much as 15 g/rat/day, while drinking ad libitum. In the control group, the rats were given solvent extract, group II, III and IV were given fraction of n-hexane of Kebar grass extract at 1 mg/kg BW, 5 mg/kg BW and 10 mg/kg BW peroral, respectively. Test preparation was given daily for 28 days, observation of toxic symptoms was performed every day while weighing was performed once every two weeks. Based on results obtained in this line of research, the findings indicated that Kebar extract administration for 28 days did not cause toxic symptoms in both male and female rats either in dose II, III or IV. Weight and ADG of male and female rats did not indicate significant differences between the control group and the treatment group.


Keywords:  kebar grass, rats, subcronic toxicity test, OECD 407

Full Text:



Bogoriani, N.W. 2008. Isolasi dan Identifikasi Glikosida Steroid dari Daun Andong (Cordyline terminalis Kunth). Jurnal Kimia 2 (1).40-44.

BPOM. 2014. Pedoman Uji Toksisitas Nonklinis secara in vivo. Badan Pengawas Obat dan Makanan RI.

Chapagain, B.P., and Wiesman, Z. 2005. Larvicidal Activity of the Fruit Mesocarp Extract of Balanites aegyptiaca and its Saponin Fractions against Aedes aegypti. B. Dengue. 29.

Kakade, R.T., Sandu, N and Senthilkumar, K.L. 2015. Evaluation of hypolipidemic and anti-obesity activities of Biophytum sensitivum Linn extracts on high fat diet induced hyperlipidemic rats. IJPCBS, 5(1): 357-360.

Lathaa, B.P., reddya, I.R.M., Vijaya, T., Raoc, S.D., Ismaild, S.M. and Girisha, B. 2011. Effect of saponin rich extract of Achyranthes aspera on high fat diet fed male wistat rats, J. Pharm. Res. 4: 3190-3193.

Marrelli, M., Conforti, F., Araniti, F. And Statti, G.A. 2016. Effects of saponins on lipid metabolism: a review of potential health benefits in the treatment of obesity, Molecules. 21(10) 1404.

OECD. 2008. OECD 407. Guidelines for the Testing of Chemicals. Repeated Dose 28-Day Oral Toxicity Study in Rodents.

Odoh, U.E., Ndubuokwu, R.I., Insya-Agha, S.I., Osadebe, P.O., Uzor, P.F. and Ezzejiofor, M. 2014. Antidiabetic activity and phytochemical screening of Acalypha wilkesiana (Euphorbiaceae) Mull Arg. roots in alloxan-induced diabetic rats. Sci. Res. Essays. 9(7): 204-212.

Oraon A. and Sinha B.N. 2012. Sub chronic toxicity potential of the alcoholic extract of Biophytum reinwardtii whole plant. Journal of Natural Sciences Research. 2(6): 94-100.

Renuka, C., Elavarasi, S., Saravanan, K and Revathi, G. 2015. Antihyperlipidemic activity of Biophytum sensitivum extracs in streptozotocin (STZ) induced diabetic albino rats, Int J Pharm Bio Sci. 6(4): 128-135.

Sambodo, P., Tethool, A.N. dan Rumetor, S.D. 2015. Efek antikolesterol fraksi n-Heksana rumput Kebar pada hewan model hiperlipidaemia. Jurnal Kedokteran Hewan. 9 (1): 59-60.

Yunita E.A., Suprapti N.H. dan Hidayat J.W. 2009. Pengaruh Ekstrak daun Teklan (Eupatorium riparium) terhadap Mortalitas dan Perkembangan Larva Aedes aegypti. BIOMA. 11. 1.


Article Metrics

Abstract views : 1751 | views : 1171


  • There are currently no refbacks.

Journal of Food and Pharmaceutical Sciences (ISSN: 2339-0948) -  Universitas Gadjah Mada, Indonesia.