Optimum Dose and Formulation of Centella asiatica L. Urban Extract Against IgG of Wistar Strain Male Mices which Induced by BCG Vaccine

https://doi.org/10.14499/jfps

Dian Eka Ermawati(1*), Ediati Sasmito(2), Mufrod Mufrod(3), Pramitha Esha ND.(4), Ni Putu UA.(5), Anggi KD(6), Muchammad Muchammad H.(7), Aini Aini S.(8)

(1) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(2) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(3) Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(4) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(5) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(6) Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(7) Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(8) Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
(*) Corresponding Author

Abstract


Objective: Centella asiatica L.Urban contains triterphenoid saphonin, flavonoid, and pectin that believed to have an immunomodulatory effect toward the Immunoglobulin-G in Wistar strain male mices which Induced by BCG vaccine. A study was conducted to find out the optimum dose and formulation of in enhancing on the level of IgG. It’s therefore expected that Cantella asiatica L.Urban can be used as an immunomodulatory suplement in both animals and human.

Methods: Centella asiatica L.Urban were macerated with ethanol 50%. This was an experimental study with the post-test control group design. The samples in this study were 35 Wistar strain male mices which divided into 7 groups: negative control [Aquadest 0,5mL/20g BW]; control immunosupresant [Prednison® 0,06mg/20g BW]; control immunostimulant [Levamisol® 0,445mg/20g BW]; ethanolic extract of Cantella asiatica L.Urban with a dose of 50mg; 100mg; 150mg; 200mg/Kg B.W were administered orally fo 10 day, after the treatments implemented, BCG [Bacillus Calmette Guerin] vaccine was infected intraperitoneally on day 10th, 14th, 21st, and 28th. The most effective doses of ethanolic extract of Cantella asiatica L.Urban was further studied for its effect on hormonal assay using ELISA reader which compared with the effects of Prednison® and Levamisol®. Based on simplex lattice design to find the optimum proportion of coefficients a, b, and ab of the equation Y = a (A) + b (B) + ab (A) (B), where A is lactose and B is amylum.

Results : Oral administration of ethanolic extract of Cantella asiatica L.Urban at dose of 50mg and 100mg/Kg B.W increased the immunity which showed elevated levels of IgG in the blood serum in Wistar strain male mices that had been induced by BCG vaccine. The optimum formula capsul of Centella asiatica L.Urban extract that use combination of 80% Amylum and 20% Lactose which produce the optimum parameters of disintegration time (≤15minutes) and CV of weight uniformity (≤5%)with the test results had no significant difference to the prediction results of Simplex Lattice Design [p>0.05].

 

Keywords:  Centella asiatica L.Urban extract, Immunomodulator, simplex lattice design


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References

Achyranthes aspera extract on phagocytosis by human neutrophils. Journal of Natural Remedies, 6: 115-119, (2006).

Bolton, S. dan Bon, C., 2003. Pharmaceutical Statistics: Practical and Clinical Applications, Revised and Expanded, 4 edition. ed. CRC Press, New York.

Di Carlo E. J., Haynes L. J., Sliver N. J.,Phillips G. H., Reticuloendothelial System Stimulants of Botanical Origin. Journal of Reticuloendothelial Society, 1: 224-232, (1964).

European Pharmacopoeia (Ph. Eur.). Council of Europe, Strasburg.2001.

Jayathirtha MG and Mishra SH. 2004. Preliminary immunomodulatory activities of methanol extracts of Eclipta alba and Centella asiatica. Phytomedicine 11: 361–365,http://www.elsevier.de/phymed.

Junqueira, Luiz C.; Jose Carneiro (2003). Basic Histology. McGraw-Hill. ISBN 0-8385-0590-2.

Kusumaningtyas, E., Sukmawati, L., Astuti, E. 2008. Journal : Evaluation of Group of Alpinia galanga n-hexan extract against Candida albicans by Bioautography and Thin Layer Chromatography. JITV 13 (4) : 323-328.

Lequin, RM (2005). Enzyme Immunoassay (EIA)/Enzyme-Linked Immunosorbent Assay (ELISA). Clinical Chemistry 51 (12): 2415–2418.

Mali R. G., Hundiwale J. C., Gavit R. S., Patil K. S., Kulkarni M. V., Effect of Int J Pharm Bio Sci 2013 Jan; 4(1): (P) 135 – 149 This article can be downloaded from www.ijpbs.net P – 149.

Plohman B., Bader G., Streich S., Hiller K., Franz G., Immuno-modulatory effects of triterpenoid saponins. European Journal of Pharmaceutical Sciences, 21: 120, (1994).

Polish Pharmacopoeia IX. Polish Pharmaceutical Society. Warsaw 2011.

Rao KGM, Rao SM and Rao SG, 2006. Centella asiatica (L.) leaf extract treatment during the growth spurt period enhances hippocampal CA3 neuronal dendritic arborization in Rats. Original Article. Advance Access Publication 14 June 2006. doi:10.1093/ecam/nel024 eCAM 2006;3(3)349–357.

Tuberculosis Fact. WHO. October 2015. Retrieved 11 February 2016.

Tuberculosis. World Health Organization. 2002.



DOI: https://doi.org/10.14499/jfps

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Journal of Food and Pharmaceutical Sciences (ISSN: 2339-0948) -  Universitas Gadjah Mada, Indonesia.