Evaluation of oxidative stress levels using glutathione peroxidase (GPx) expression on hyperglycemia-induced rats testis

https://doi.org/10.19106/JMedSci005304202103

Andreas Pramudito(1), Sakti Ronggowardhana Brodjonegoro(2*), Ahmad Zulfan(3), Aria Danurdoro(4), Didik Setyo Heriyanto(5)

(1) Divison of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta
(2) Divison of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta
(3) Divison of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta
(4) Divison of Urology, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta
(5) Department of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta
(*) Corresponding Author

Abstract


Diabetes mellitus (DM) is a global health problem with an estimated 422 million cases worldwide. Previous studies reported a correlation between hyperglycemia and oxidative stress‐related male infertility in DM. Glutathione peroxidase (GPx) can cause DNA damage due to oxidative reactions. Therefore, it could be used as potential indicator of antioxidant therapy. The study aimed to evaluate the expression level of GPx on the hyperglycemia-induced rats. This was an experimental case-control study using 27 Wistar rats divided into three groups i.e. hyperglycemia induction for four weeks group, eight weeks group, and a control group with no intervention. Following after induction, total RNA from the rats' testis was extracted, and GPx expression was analyzed using qPCR. Data were analyzed using SPSS, and a p <0.05 was considered significant. The study showed a significantly higher GPx mRNA expression level after hyperglycemia induction in both 4 and 8 weeks groups (16.93 ± 3.32 and 17.62 ± 3.42) compared to control group (9.94 ± 2.91) (p< 0.05). However, no significantly different between the 4 weeks group and 8 weeks group was observed (p >0.05). In conclusion, hyperglycemia increases GPx mRNA expression in rats. It may change the testicular environment's oxidative processes and impairs male reproductive function in the Sertoli cells with no exception.


Keywords


GPX; mRNA; expression; hyperglycemia; antioxidant

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References


  1. NCD Risk Factor Collaboration (NCD-RisC). Worldwide trends in diabetes since 1980: a pooled analysis of 751 population-based studies with 4.4 million participants. Lancet 2016; 387(10027):1513-30. https://doi.org/10.1016/S0140-6736(16)00618-8
  2. Saeedi P, Petersohn I, Salpea P, Malanda B, Karuranga S, Unwin N, et al. On behalf of the IDF Diabetes Atlas Committee. Global and regional diabetes prevalence estimates for 2019 and projections for 2030 and 2045: Results from the International Diabetes Federation Diabetes Atlas. Diabetes Res Clin Pract 2019; 157:107843. https://doi.org/10.1016/j.diabres.2019.107843
  3. Hwang TIS, Tsai TF, Lin YC, Chiang HS, Chang LS. A survey of erectile dysfunction in Taiwan: use of the erection hardness score and quality of erection questionnaire. J Sex Med 2010; 7(8):2817-24. https://doi.org/10.1111/j.1743-6109.2010.01837.x
  4. Samadian Z, Tofighi A, Razi M, Tolouei Azar J, Pakdel FG. Moderate-intensity exercise training ameliorates the diabetes-suppressed spermatogenesis and improves sperm parameters: insole and simultaneous with insulin. Andrologia 2019; 51(11):1-11. https://doi.org/10.1111/and.13457
  5. Roessner C, Paasch U, Kratzsch J, Glander HJ, Grunewald S. Sperm apoptosis signalling in diabetic men. Reprod Biomed Online 2012; 25(3):292-9. https://doi.org/10.1016/j.rbmo.2012.06.004
  6. Lao-Ong T, Chatuphonprasert W, Nemoto N, Jarukamjorn K. Alteration of hepatic glutathione peroxidase and superoxide dismutase expression in streptozotocin-induced diabetic mice by berberine. Pharm Biol 2012; 50(8):1007-12. https://doi.org/10.3109/13880209.2012.655377
  7. Bhattacharya SM, Ghosh M, Nandi N. Diabetes mellitus and abnormalities in semen analysis. J Obstet Gynaecol Res 2014; 40(1):167-71. https://doi.org/10.1111/jog.12149
  8. Abd El-Twab SM, Mohamed HM, Mahmoud AM. Taurine and pioglitazone attenuate diabetes-induced testicular damage by abrogation of oxidative stress and up-regulation of the pituitary-gonadal axis. Can J Physiol Pharmacol 2016; 94(6):651-61. https://doi.org/10.1139/cjpp-2015-0503
  9. Aitken RJ, Roman SD. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev 2008; 1(1):15-24. https://doi.org/10.4161/oxim.1.1.6843
  10. Ernawati, I’tishom R, Sudjarwo SA. Role of MDA, SOD and GPx expression on protective mechanism of xanthone against 2-methoxyethanol-decreased number of sertoli cell in mice. Interciencia J 2020; 45(1):104-14.
  11. Carpino A, Rago V, Guido C, Casaburi I, Aquila S. Insulin and IR-β in pig spermatozoa: A role of the hormone in the acquisition of fertilizing ability. Int J Androl 2010; 33(3):554-62. https://doi.org/10.1111/j.1365-2605.2009.00971.x
  12. DeFronzo RA, Ferrannini E, Groop L, Henry RR, Herman WH, Holst JJ, et al. Type 2 diabetes mellitus. Nat Rev Dis Prim 2015; 1:15019. https://doi.org/10.1038/nrdp.2015.19
  13. Ding GL, Liu Y, Liu ME, Pan JX, Guo MX, Sheng JZ, et al. The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis. Asian J Androl 2015; 17(6):948-53. https://doi.org/10.4103/1008-682X.150844
  14. Viloria T, Meseguer M, Martínez-Conejero JA, O'Connor JE, Remohí J, Pellicer A, et al. Cigarette smoking affects specific sperm oxidative defenses but does not cause oxidative DNA damage in infertile men. Fertil Steril 2010; 94(2):631-7. https://doi.org/10.1016/j.fertnstert.2009.03.024
  15. Zhang JX, Wang ZM, Zhang JJ, Zhu LL, Gao XF, Chen SL. Association of glutathione peroxidase-1 (GPx-1) rs1050450 Pro198Leu and Pro197Leu polymorphisms with cardiovascular risk: a meta-analysis of observational studies. J Geriatr Cardiol 2014; 11(2):141-50. https://doi.org/10.3969/j.issn.1671-5411.2014.02.003
  16. Omolaoye Temidayo S, Du Plessis Stefan S. Diabetes mellitus and male infertility. Asian Pacific J Reprod 2018; 7(1):6-14. https://doi.org/10.4103/2305-0500.220978
  17. Lee J, Lee DR, Lee S. The genetic variation in monocarboxylic acid transporter 2 (MCT2) has functional and clinical relevance with male infertility. Asian J Androl 2014; 16(5):694-7. https://doi.org/10.4103/1008-682X.124561
  18. Mazjin MA, Salehi Z, Mashayekhi F, Bahadori M. Evaluation of GPx1 Pro198Leu polymorphism in idiopathic male infertility. Mol Biol 2016; 50(1):77-80. https://doi.org/10.1134/S0026893316010143
  19. Sulastri, Ernawati, Sudjarwo SA. The role of glutathione peroxidase expression in the protective effect of xanthone on mice sertoli cell number induced by 2- methoxyethanol. Int J Med Sci Innov Res 2020; 5(4):76-81.



DOI: https://doi.org/10.19106/JMedSci005304202103

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