Effects dadih on mencit balb / c based on activities of cytokine Pro-inflammation and anti-inflammation



Ria Kodariah(1*), Hadits Lissentiya Armal(2), Heri Wibowo(3), Andi Yasmon(4)

(1) Department of Anatomical Pathology Medical Faculty Universitas Indonesia
(2) Student of Immunology Magister Program of Biomedical Science Medical Faculty Universitas Indonesia
(3) Department of Parasitology Medical Faculty Universitas Indonesia
(4) Department of Microbiology Medical Faculty, Universitas Indonesia
(*) Corresponding Author

Abstract


Normal microflora form of commensal bacteria has the role to maintain homeostasis in the intestine tract. The reduction amount and diversity of commensal bacteria lead dysbiosis in gastrointestinal are followed by an increase of pathogens to induce inflammatory conditions to develop become colorectal cancer. Probiotics make as defensive, regulation and modulation the immune response by triggering the development of pathogen-specific memory. Currently, many foreign probiotic products are circulating in the market so the domestic products are less well known and less attractive. Dadih is a probiotic product originating from West Sumatra, Indonesia is made from fermented buffalo milk containing Lactic Acid Bacteria (LAB). Our aim in this study was to investigate effect dadih in maintaining intestinal tract homeostasis. The study was conducted using male Balb /c mice aged 6-8 weeks with weighing 20-30 grams, is given dadih at doses 112 mg / 20gBB for 8 weeks. The results indicated that bacterial BAL contained in dadih is coccus, gram-positive bacteria having a number of 2x108 colony-forming units (CFU) dominated by Lactococcus lactis subsp. lactic. The results of measurements of cytokines showed increase anti-inflammatory cytokines IL-10 level and proinflammatory cytokines TNF-alpha and IL-1β level. This indicated dadih as a local probiotic can modulate the immune system by maintaining a balance of pro-inflammatory and anti-inflammatory cytokine level production.

Keywords


Dadih, Probiotics, IL-1β, IL-10, TNF-alpha





Article Metrics

Abstract views : 172




Copyright (c) 2019 Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

View My Stats

 

Creative Commons License
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by  Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Based on a work at http://jurnal.ugm.ac.id/bik/.