Role of methylprednisolone on mRNA expression of BAX, BCL-2 genein testicular torsion-detorsion of male albino Wistar rats

https://doi.org/10.19106/JMedSci005203202002

Muhammad Puteh Mauny(1*), Raden Danarto(2), Didik Setyo Heriyanto(3)

(1) Urology Division, Department of Surgery Faculty of Medicine, Public Health and Nursing Universitas Gadjah Mada/Dr. Sardjito Hospital, Yogyakarta, Indonesia
(2) Urology Division, Department of Surgery Faculty of Medicine, Public Health and Nursing Universitas Gadjah Mada/Dr. Sardjito Hospital, Yogyakarta, Indonesia
(3) Department of Anatomical Pathology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital, Yogyakarta, Indonesia.
(*) Corresponding Author

Abstract


Study Background : Testicular torsion is a genitourinary emergency most common in children and emergencies requiring second surgery in adolescents after acute appendicitis1. Testicular torsion is more common on the left with a 1.2: 1 ratio, which is probably caused by a slightly longer spermatic cord on the left2. Testicular torsion can occur at any age, but the peak incidence is at age 14, with a peak of second occurrence at 1 year of age3. At 1 year of age, testicular torsion is a major cause of acute scrotum (83%). At the age of 3 - 13 years, the most frequent diagnosis is the torsion of the testicular appendix. After the age of 17 years, epididymitis is the most frequent diagnosis (75%)4.

Method: This study is an experimental study, post-test only control group design, the sample was randomly divided into 4 groups, 2 intervention groups and 2 control groups. In this study the independent variables are the duration of the right torsion-detection treatment of the testes and the injection of Methylprednisolone in Wistar male rats. The dependent variables are mRNA expression in apoptosis and anti-apoptosis genes in rats’ ipsilateral and contralateral testes.
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Results and Discussion: In this study, two-way ANOVA is used to analyze data between groups. Significant decrease in BAX gene mRNA expression (p <0.05) with Methylprednisolone was present when group 2 (TD) was compared with group 4 (TD-MP). Significant increase in the expression of BCL-2 anti-apoptotic gene (p <0.05) with Methylprednisolone was present when group 1 (T) was compared with group 3 (T-MP). When group 2 (TD) compared with group 4 (TD-MP) also showed a significant increase in the expression of BCL-2 anti-apoptotic gene (p <0.05).

Conclusion: The administration of Methylprednisolone in the case of testicular torsion proved to be significant in increasing the expression of the BCL-2 gene that could be a protective mechanism against germ cell apoptosis in testicular tissue.

Keywords


Testicular torsion, Germ cell apoptosis, Methylprednisolone

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References

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DOI: https://doi.org/10.19106/JMedSci005203202002

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Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by  Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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