The protective role of single bulb garlic (Allium sativum L.) towards foam cells and vascular cell adhesion molecules-1 (VCAM-1) on mice (Mus musculus) with high-fat diet

https://doi.org/10.19106/JMedSci005103201905

Isfatun Chasanah(1), Abdul Ghofur(2), Sri Rahayu Lestari(3*)

(1) Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Negeri Malang
(2) Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Negeri Malang
(3) Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Negeri Malang
(*) Corresponding Author

Abstract


 

Atherosclerosis is initiated by inflammatory response characterized by the accumulation of lipids in the arteries, causing blockage of blood vessels. The excessive of low-density lipoprotein (LDL) in the blood is a major factor in endothelial dysfunction and results in an inflammatory process. The inflammatory process in atherosclerosis is due to the expression of adhesion molecules, vascular cell adhesion molecules-1 (VCAM-1). This research aimed to evaluate the effect of single bulb garlic oil extract (SGBOE) against VCAM-1 expression in high-fat diet mice. Thirty male mice strain Balb/C were acclimatized for one week. Mice were divided into 6 groups (n=5): normal, high-fat diet (HFD), HFD + simvastatin, and HFD + SBGOE 12.5, 25, and 50 mg/kg BW respectively. HFD was given for 45 days. The SGBOE was given for four weeks orally. The aortic VCAM-1 expression was assayed using the immunohistochemical-flouresencemethod. The result of One-Way ANOVA showed that the treatment influenced significantly (p<0.05). The foam cells increase in the high-fat diet group compared than normal mice. Dose 12,5 mg/kg BW reduces VCAM-1 expression near the normal group. Allicin and S-allyl cysteine (SAC) compounds in single bulb garlic are antioxidant compounds that act to prevent oxidative stress and prevention mechanisms against lipoprotein modification.


Keywords


atherosclerosis; single bulb garlic oil extract; VCAM-1

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References

  1. Dahlöf B. Cardiovascular disease risk factors: epidemiology and risk assessment. Am J Cardiol 2010; 105(1 SUPPL.):3A-9A. https://doi.org/10.1016/j.amjcard.2009.10.007.
  2. WHO. Cardiovascular diseases (CVDs). http://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds). Published 2017. Accessed November 7, 2018.
  3. Kementerian Kesehatan RI PD dan I. Situasi kesehatan jantung dalam Mari menuju masa muda sehat, hari tua nikmat tanpa PTM dengan perilaku cerdik. Jakarta: Kementrian Kesehatan Republik of Indonesia, 2014.
  4. Bentzon JF, Otsuka F, Virmani R, Falk E. Mechanisms of plaque formation and rupture. Circ Res 2014; 114(12):1852-1866. https://doi.org/10.1161/CIRCRESAHA.114.302721.
  5. De Winther MPJ, Kanters E, Kraal G, Hofker MH. Nuclear factor κB signaling in atherogenesis. Arterioscler Thromb Vasc Biol 2005; 25(5):904-14. https://doi.org/10.1161/01.ATV.0000160340.72641.87.
  6. Jamkhande PG, Chandak PG, Dhawale SC, Barde SR, Tidke PS, Sakhare RS. Therapeutic approaches to drug targets in atherosclerosis. Saudi Pharm J 2014; 22(3):179-90. https://doi.org/10.1016/j.jsps.2013.04.005.
  7. Singh U, Kumar S, Dhakal S. Future prospect of garlic usage in clinical practice of hyperlipidemia : a review. Int J Herb Med 2015; 3(2):38-43.
  8. Hansson GK, Hermansson A. The immune system in atherosclerosis. Nat Immunol. 2011; 12(3):204-12. https://doi.org/10.1038/ni.2001.
  9. 9. Preiss DJ, Sattar N. Vascular cell adhesion molecule-1: a viable therapeutic target for atherosclerosis? Int J Clin Pract 2007; 61(4):697-701. https://doi.org/10.1111/j.1742-1241.2007.01330.x.
  10. Esenwa CC & Elkind MS. Inflammatory risk factors, biomarkers and associated therapy in ischaemic stroke. Nat Rev Neurol 2016; 12(10):594-604. https://doi.org/10.1038/nrneurol.2016.125.
  11. Farmawati A, Kusuma RJ, Iswara BS, Utami KD. Addition of conjugated linoleic acid in whole milk improves lipid profile in high fat diet induced hypercholesterolemia of rats. J Med Sci 2016; 48(4):185-92. https://doi.org/10.19106/JMedSci004804201601.
  12. Dalal I, Sengupta M, Paul S, Mishra A. Comparative study of the effect of atorvastatin and garlic extract in experimentally induced hypercholesterolemia in rabbits. Int J Basic Clin Pharmacol 2013; 2(4):397. https://doi.org/10.5455/2319-2003.ijbcp20130810.
  13. Colín-González AL, Santana RA, Silva-Islas CA, Chánez-Cárdenas ME, Santamaría A, Maldonado PD. The antioxidant mechanisms underlying the aged garlic extract- and S-allylcysteine-induced protection. Oxid Med Cell Longev 2012; 2012(1). https://doi.org/10.1155/2012/907162.
  14. Molina-Calle M, de Medina VS, Priego-Capote F, de Castro MDL. Establishing compositional differences between fresh and black garlic by a metabolomics approach based on LC–QTOF MS/MS analysis. J Food Compos Anal 2017; 62:155-63. https://doi.org/10.1016/j.jfca.2017.05.004.
  15. Murwani S, Ali M, Muliartha K. Atherogenetic diet of wite rat (Rattus novergicus strain Wistar) as aterosclerotic animal model. Jurnal Kedokteran Brawijaya 2006; 22(1):6-9. https://doi.org/10.21776/ub.jkb.2006.022.01.2
  16. Pujiastuti INE, Lestari SR, Gofur A. Gambaran hematologi mencit (Mus musculus) model toksisitas subkronis. Scr Biol 2017; 4(2):75. https://doi.org/10.20884/1.sb.2017.4.2.371.
  17. Chung LY. The antioxidant properties of garlic compounds: allyl cysteine, alliin, allicin, and allyl disulfide. J Med Food 2006; 9(2):205-13. https://doi.org/10.1089/jmf.2006.9.205.
  18. Rahman MM, Fazlic V, Saad NW. Antioxydant proprietés of raw garlic (Allium sativum) extract. Int Food Res J 2012; 19(2):589-91.
  19. Gonen A, Harats D, Rabinkov A, Miron T, Mirelman D, Wilchek M, et al. The antiatherogenic effect of allicin: possible mode of action. Pathobiology 2006; 72(6):325-34. https://doi.org/10.1159/000091330.
  20. Gutowski M, Kowalczyk S. A study of free radical chemistry: their role and pathophysiological significance. Acta Biochim Pol 2013; 60(1):1-16. https://doi.org/10.18388/abp.2013_1944
  21. Sayuti K & Yenrina R. Antioksidan alami dan sintetik. Padang: Andalas Univrsity Press, 2014.
  22. Ross R. Atherosclerosis - an inflammatory disease. N Engl J Med 1999; 340(2):115-26. https://doi.org/10.1056/NEJM199901143400207.
  23. Rader DJ, Daugherty A. Translating molecular discoveries into new therapies for atherosclerosis. Nature 2008; 451(7181):904-13. https://doi.org/10.1038/nature06796.
  24. Amal AF, Sanaa TE-S. Effect of Allium sativum extract on serum lipid and antioxidant status in hypercholesterolemic rabbits. Life Sci J 2012; 9(3):187-96.
  25. Al-Numair KS. Hypocholesteremic and antioxidant effect of garlic (Allium sativum L.) extract in rats fed high cholesterol diet. Pakistan J Nutr 2009; 8(2):161-6. https://doi.org/10.3923/pjn.2009.161.166.



DOI: https://doi.org/10.19106/JMedSci005103201905

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