Correlation between micro-RNA-21 expression and Inflammation Cytokine in Rabbits Implanted with Bare Metal Stent with the Incidence of Neo Intimal Hyperplasia

Hariadi Hariawan(1*), Marsetyawan HNE Soesatyo(2), Muhammad Munawar(3), Ahmad Ghozali(4)

(1) Department of Cardiology and Vascular Medicine FKKMK UGM Yogyakarta
(2) Department of Histology and Cell Biology FKKMK UGM Yogyakarta
(3) Bina Waluya Cardiac Center Jakarta
(4) Department of Pathology FKKMK UGM Yogyakarta
(*) Corresponding Author



Background: In stent restenosis after stenting in vascular occurs secondary to the accumulation of smooth muscle cell and extracellular matrix. This condition is a major complication caused by the occurrence of neointimal hyperplasia (NIH). Aim: To prove the role of miRNA-21 as a risk predictor of the NIH  event in aorta rabbits animal model who underwent BM type stent implantation and to know the miRNA-21 role in IL-6 and IL-8 expressions.

Methods: This study is quasy experimental, had been done in Cath Laboratory Sardjito General Hospital Yogyakarta. Stent implantation with observe intravascular ultrasonography. Blood vessel which is already deployed by stent will be taken as sample for pathologic examination. Rabbit blood samples were collected on  day 0, 7, and 28. Quantification expression has been done with qPCR and Elisa approach. Results: These 10 rabbits divided into two groups in which one group as control (without stent deployment, 4 models) and another group as intervention (with BM type stent deployment, 6 models). The increase in expression of miRNA-21 on the day 7 and day 28 in the intervention group compared to the control group. NIH are increase from control group and in intervention group on day 7, and 28 are from mild grade to moderate, and severe respectively. Also an  increasing levels of IL-6 and IL-8 from day 7 to day 28. Conclusion: This study shows there is an increase of miRNA-21 expression on day 7 and NIH  rise from the mild to moderate. Furthermore, on day 28, that increase of miRNA-21 expression and NIH  rise from to moderate to severe. The increase of miRNA-21 is also shown on day 7 and day 28 with increase of IL-6 and IL-8 value.


miRNA-21; bare metal stent ; interleukin-6; interleukin-8; neo intimal hyperplasia

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Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by  Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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