mRNA expression of CYP17A1, CYP11A1, CYP19A1, HSD3B1 and AKR1C2 in metastatic and non-metastatic prostate cancer patients



Indrawarman Soerohardjo(1*), Muhammad Puteh Mauny(2), Alharsya Franklyn Ruckle(3), Ahmad Zulfan(4), Raden Danarto(5), Didik Setyo Heriyanto(6)

(1) Urology Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia
(2) Urology Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia
(3) Urology Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia
(4) Urology Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia
(5) Urology Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia
(6) Urology Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


The progression of prostate cancer (PCa) mainly occurs caused by androgens. There is a link

between intratumoral steroidogenesis and castration-resistant prostate cancer. This study
aimed to determine the mRNA expression of various steroidogenic enzymes (CYP17A1,
CYP11A1, CYP19A1, HSD3B1, and AKR1C2) in metastatic and non-metastatic prostate
cancer patients. This study was conducted at the Anatomical Pathology Laboratory and
Urologi Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing,
Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta from September-
November 2017. Samples were taken from 30 paraffin blocks with adenocarcinoma of
prostate, stained with hematoxylin-eosin (HE) and then classified into metastatic and nonmetastatic
groups. Samples then underwent deparaffinization procedure and examination
of mRNA expression of CYP17A1, CYP11A1, CYP19A1, HSD3B1, AKR1C2 genes using
Real-Time PCR. The mean mRNA expressions of CYP11A1, CYP17A1, CYP19A1,
HSD3B1, and AKR1C2 genes in the metastatic adenocarcinoma prostate group were 7.08,
10.11, 3.94, 4.84 and 3.58, respectively. In the non-metastatic group, the mean mRNA
expressions of CYP11A1, CYP17A1, CYP19A1, HSD3B1, and AKR1C2 genes were 4.62,
9.45, 3.46, 2.68 and 4.92, respectively. The mean of mRNA expression of CYP11A1,
CYP17A1, CYP19A1, and HSD3B1 genes were higher in the metastatic group than nonmetastatic
adenocarcinoma prostate group. However, it was not statistically significant
(p>0.05). The highest mRNA expression of steroidogenic enzymes was the CYP17A1
gene. In conclusion, the mRNA expressions of CYP17A1, CYP11A1, CYP19A1, HSD3B1
were higher in the metastatic prostate cancer patients compared to that in non-metastatic
prostate cancer patients but statistically not significant.


Keywords


Prostate cancer - CYP17A1 - metastatic - non-metastatic - steroidogenesis, mRNA

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References

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Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by  Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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