Expression of circulating miR-200c and vascular endothelial growth factor-A (VEGF-A) mRNA as potential biomarker in human hepatocellular carcinoma



Nanda Qoriansas(1*), Dede Renovaldi(2), Juwita Raditya(3), Puji Lestari(4), Nur Signa Gumilas(5), . Suharno(6), Didik Setyo Heriyanto(7), Neneng Ratnasari(8), Sofia Mubarika H(9)

(1) Basic Medical Science and Biomedicine Program Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(2) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(3) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(4) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(5) Faculty of Medicine, UNSOED Purwokerto Indonesia
(6) Faculty of Medicine, UNSOED Purwokerto Indonesia
(7) Department of Anatomic Pathologiy, Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(8) Department of Internal Medicine, Universitas Gadjah Mada Yogyakarta
(9) UGM Faculty of Medicine, Universitas Gadjah Mada Yogyakarta
(*) Corresponding Author

Abstract


Hepatocellular carcinoma (HCC) is a common liver disease that causes significant public
health problems throughout the world, including in Indonesia. The HCC is the six most
common cancers and second cancer-related deaths among men in the world. Recently
it was reported that the microRNA is an important player in hepatocarcinogenesis. The
expression of MiRNA-200c is often regulated in primary HCC and HCC cell lines. Vascular
endothelial growth factor-A (VEGF-A) is a regulator of angiogenesis that has been reported
as miR-200c target gene. This study was conducted to measure expression levels in miR-
200c and mRNAVEGF-A and their potential role as biomarkers at HCC. A total of 36
HCC patients and 36 healthy subjects were included in this study. The relative expression
of miRNA-200c and mRNA VEGF-A was quantified using reverse transcription real time
quantitative PCR (qRT PCR). Relative expression was calculated using . Unpaired t-test
was used to compare the expression levels of circulating miRNA-200c and mRNA VEGF-A
in HCC patients and healthy subjects. Pearson test was used to determine correlation
between circulating miR-200c expression and mRNA VEGF-A expression levels. The
expression levels of circulating miR-200c in HCC patients were lower compared to healthy
subjects although it was not significant (p = 0.258). Conversely, the expression levels
of circulating mRNA VEGF-A in HCC patients were significantly higher compared to
healthy subjects (p = 0.001). The relative expression levels of circulating miR-200c were
negatively correlated with mRNA VEGF-A in HCC patients. In conclusion, the expression
levels of mRNA VEGF-A in HCC patients are significantly deregulated in compared to
that in healthy subjects. Negative correlation between circulating miRN-200c and mRNA
VEGF-A expression levels are reported in HCC patients.


Keywords


blood plasma - hepatocellular carcinoma - miR-200c - mRNA VEGF-A - biomarker

Full Text:

PDF




Article Metrics

Abstract views : 158 | views : 90




Copyright (c) 2019 Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

View My Stats

 

Creative Commons License
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by  Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Based on a work at http://jurnal.ugm.ac.id/bik/.