The effect of L-arginine therapy on hepatocyte cells damage in mice (Mus musculus) model of preeclampsia

https://doi.org/10.19106/JMedSci005104201905

S Soetrisno(1*), Bambang Arinekso(2), Sri Sulistyowati(3)

(1) Department of Obstetrics and Gynecology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta, Indonesia
(2) Department of Obstetrics and Gynecology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta, Indonesia
(3) Department of Obstetrics and Gynecology, Faculty of Medicine, Sebelas Maret University/Dr. Moewardi General Hospital, Surakarta, Indonesia
(*) Corresponding Author

Abstract


Preeclampsia is one of the main contributors on morbidity and mortality of mother and fetus. Imbalance of body antioxidants and oxidants has a role on endothelial destruction causing endothelial dysfunction which leads to preeclampsia. L-arginine administration is indicated to be able to fix the remodelling process of hepatocytes which was damaged on preeclampsia. The aim of this study was to investigate the effect of L-arginine on hepatocyte cells damage on mouse (Mus musculus) model of preeclampsia. Experimental study was conducted in the Experimental Animal Warehouse and Biomedical Laboratory, Faculty of Veterinary Medicine, Airlangga University, Surabaya. A total of 30 pregnant mice were divided into three groups with 10 mice in each group i.e. normal, preeclampsia model and preeclampsia model with L-arginine therapy pregnant mice. Preparates were taken from dissected mice liver, with prior paraffin block and were then stained with hematoxylin-eosin. Results were analyzed semi quantitatively with Manja Roenigk hepar histology scoring method. Data were analyzed using Kruskal-Wallis and Man-Whitney test. Mean of hepar histopathology score of preeclampsia model with L-arginine therapy (40.80±1.17/µm2) was significantly lower compared with preeclampsia model pregnant mice (58.80±4.23/µm2) (p<0.05), however it was significantly higher compared with normal pregnant mice (18.40±2.41/µm2) (p<0.05). It was indicated that the L-arginine therapy on preeclampsia mouse model can reduce the score of hepar histopathology. In conclusion, L-arginine administration can reduce the hepatocyte cells damage of mouse due to preeclampsia.


Keywords


L-arginine; hepatocytes cell; preeclampsia; mouse model; animal model

Full Text:

PDF


References

1. Bilano VL, Ota E, Ganchimeg T, Mori R, Souza JP. Risk factors of preeclampsia/eclampsia and its adverse outcomes in low- and middle-income countries: a who secondary analysis. PLoS One 2014; 9(3):e91198.
https://doi.org/10.1371/journal.pone.0091198
2. Staff AC, Benton SJ, von Dadelszen P, Roberts JM, Taylor RN, Powers RW, et al. Redefining preeclampsia using placenta-derived biomarkers. Hypertension 2013; 61(5):932-42.
https://doi.org/10.1161/HYPERTENSIONAHA.111.00250
3. Sulistyowati S, Abadi A, Wijiati. Low class Ib (HLA-G/Qa2) MHC protein expression against HSP-70 and VCAM-1 profile on preeclampsia: an observation on experimental animal musmusculus with endothelial dysfunction model. Indonesian J Obstet Gynecol 2010; 34(3):103-6.
4. Jankowski M. Angiogenesis inhibition and preeclampsia. J Hypertens 2009; 27(4):707-8.
https://doi.org/10.1097/HJH.0b013e3283293dc5
5. Ekambaram P. HSP70 Expression and its role in preeclamptic stress. Indian J Biochem Biophys 2011; 48(4):243-55.
6. Villar J, Purwar M, Merialdi M, Zavaleta N, thi Nhu Ngoc N, Anthony J, et al. World Health Organization multicentre randomized trial of supplementation with vitamin C and E among pregnant women at high risk for preeclampsia in populations of low nutritional status from developing countries. BJOG 2009; 116(6):780-8.
https://doi.org/10.1111/j.1471-0528.2009.02158.x
7. Camarena Pulido EE, García Benavides L, Panduro Barón JG, Pascoe Gonzalez S, Madrigal Saray AJ, García Padilla FE, et al. Efficacy of L arginine for freventing preeclampsia in high-risk pregnancies: a double-blind, randomized clinical trial. Hypertens Pregnancy 2016. 35(2):217-25.
https://doi.org/10.3109/10641955.2015.1137586
8. Pramatirta AP. Penatalaksanaan hipertensi dalam kehamilan dengan berbagai komplikasinya dalam penatalaksanaan intensif obstetri. Bandung: Sagung Seto. 2015.
9. Leveno KJ. Hipertensi gestasional dan preeklamsia dalam manual Williams komplikasi kehamilan, Edisi 23. Jakarta: EGC. 2016; I,23;210-20.
10. Angsar MD. Hipertensi dalam kehamilan. Dalam Ilmu Kebidanan Sarwono Prawirohardjo, Edisi V. Jakarta: PT Bina Pustaka Sarwono Prawirohardjo. 2014.
11. Israel A, Elsa C, Matos MG, Garrido MDR. Rat kidney antioxidant enzyme activities in experimental preeclampsia. laboratory of neuropeptides, school of pharmacy, universidad central de Venezuela, Caracas, Venezuela. IJCMPR 2015; 1(7):104-9.
12. Supranto J. Teknik sampling untuk survei dan eksperimen. Jakarta: Rineka Cipta, 2007.
13. Nursheha A, Febrianti N. Pengaruh ekstrak daun cincau hijau (cyclea barbata miers). terhadap gambaran histopatologik hepar mencit (Mus musculus) yang diinduksi MSG. JUPEMASI-PBIO 2015; 1(2):198-203.
https://doi.org/10.26555/bioedukatika.v2i1.4105
14. Mescher AL. Junqueira's basic histology text and atlas, 13th Ed. New York: McGraw-Hill Education, 2013.
15. Chan AWH, Quaglia A, Haugk B, Burt A. Atlas of Liver Pathology. New York: Springer. 2014.
https://doi.org/10.1007/978-1-4614-9114-9
16. Wall TD, Grivell RM, Dekker GA, Hague W, Dodd JM. The role of L-arginine in the prevention and treatment of pre-eclampsia: a systematic review of randomised trials. J Hum Hypertens 2014; 28(4):230-5.
https://doi.org/10.1038/jhh.2013.100.
17. Grafka A, Lopucki M, Kajszczarek KK. Study of the role L-arginine in the diagnosis of pregnancy-induced hypertension. Arterial Hypertens, 2016; 20(3):113-8.
https://doi.org/10.5603/AH.2016.0017
18. Hegde CV. The use of L-Arginine in the management of pre-eclampsia and intrauterine growth restriction. J Obstet Gynecol India 2012; 62(1):1-2.
https://doi.org/10.1007/s13224-012-0146-8.
19. De Lucca L, Gallareta FMP, Goncalves TDL. Oxidative stress markers in pregnant woman with preeclampsia. Am J Med Biol Res 2015; 3(3):68-73.
https://doi.org/10.12691/ajmbr-3-3-1
20. Vadillo-Ortega F, Perichart-Perera O, Espino S, Avila-Vergara MA, Ibarra I, Ahued R, et al. Effect of supplementation during pregnancy with L-arginine and antioxidant vitamins in medical food on pre-eclampsia in high risk population: randomised controlled trial. BMJ 2011; 342: d2901.
https://doi.org/10.1136/bmj.d2901
21. Creasy RK, Resnik R, Iams JD, Lockwood CJ, Moore TR, Greene MF. Pregnancy-related hypertension in creasy & resnik's maternal-fetal medicine principle and practice, 7th ed. Philadelphia USA: Saunders Elvesiers, 2014; Part 4, 48;756-81.
22. Kumar V, Abbas AK, Aster JC. Robbins and cotran pathologic basic of disease, 9th Ed. Philadelphia USA: Saunders Elsevier. 2015.
23. Shunping G, Jin J, Xiaoyu N, Yi B, Heng Z, Juelin D, et al. Arginine supplementation for improving maternal and neonatal outcomes in hypertensive disorder of pregnancy: a systematic review. JRAAS 2014; 15(1):88-96.
https://doi.org/10.1177/1470320313475910
24. Hegde CV. The use of L-arginine in the management of preeclampsia and intrauterine growth restriction. J Obstet Gynecol India 2012; 62(1):1-2.
http://doi.org/10.1007/s13224-012-0146-8.



DOI: https://doi.org/10.19106/JMedSci005104201905

Article Metrics

Abstract views : 1752 | views : 2996




Copyright (c) 2019 S Soetrisno, Bambang Arinekso, Sri Sulistyowati

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.