In Vitro Antiaging Analysis of Topical Pharmaceutical Preparation Containing Mixture of Strawberry Fruit, Pomelo Peel, and Langsat Fruit Extracts
Abstract
The previous research has discovered that strawberry fruit, pomelo peel and langsat fruit peel have antioxidant activity. This research aimed to put the extract combination into several formula of gel and cream. Extract will be formulated into two gels with Natrium Carboxyl Methyl Cellulose as gelling agent for Formula 1 and Synthalen for Formula 2. Whereas there are three cream formulations with Glyceril Mono Stearat as surfactant for Formula 3, Polawax for Formula 4, and Simulsol 165 for Formula 5. Natrosol HBr was added to Formula 3 as thickening agent and Keltrol CG-SFT added to Formula 5. After five formulas being made, their stability was tested using climatic chamber within 30 days. Physical stability parameters were spreadability, stickiness, pH, viscosity, separation degree, and syneresis test. The chemical properties evaluation of the formula was done by measuring radical 2,2-difenyl-1-pikrylhidrazil (DPPH) scavenging activity percentage, anti-collagenase activity, anti-tyrosinase activity, and anti-elastase activity. The results showed that all of formulas have good activity in scavenging DPPH radical, anti-elastase, anti-collagenase and anti-tyrosinase as well. In addition, all formulas have good stability. There is no change in appearance after three times freeze-thaw cycle. Therefore, it was concluded that all formula can be developed as topical preparation for antiaging.
References
2. Farage MA, Miller KW, Elsner P, Maibach HI. Structural characteristics of the aging skin: A review. Cutan Ocul Toxicol. 2007;26(4):343–57.
3. Landau M. Exogenous factors in skin aging. Curr Probl Dermatol. 2007;35:1–13.
4. Tobin DJ. Introduction to skin aging. J Tissue Viability [Internet]. 2017;26(1):37–46. Available from: http://dx.doi.org/10.1016/j.jtv.2016.03.002
5. Lephart ED. Equol’s anti-aging effects protect against environmental assaults by increasing skin antioxidant defense and ECM proteins while decreasing oxidative stress and inflammation. Cosmetics. 2018;5(1):1–17.
6. Giampieri F, Alvarez-Suarez JM, Mazzoni L, Forbes-Hernandez TY, Gasparrini M, Gonzàlez-Paramàs AM, et al. Polyphenol-rich strawberry extract protects human dermal fibroblasts against hydrogen peroxide oxidative damage and improves mitochondrial functionality. Molecules. 2014;19(6):7798–816.
7. Giampieri F, Alvarez-Suarez JM, Tulipani S, Gonzàles-Paramàs AM, Santos-Buelga C, Bompadre S, et al. Photoprotective potential of strawberry (Fragaria × ananassa) extract against UV-A irradiation damage on human fibroblasts. J Agric Food Chem. 2012;60(9):2322–7.
8. Zhou Y, Dong Y, Xu Q, He Y, Tian S, Zhu S, et al. Mussel oligopeptides ameliorate cognition deficit and attenuate brain senescence in d-galactose-induced aging mice. Food Chem Toxicol [Internet]. 2013;59:412–20. Available from: http://dx.doi.org/10.1016/j.fct.2013.06.009
9. Kim JH, Byun JC, Bandi AKR, Hyun CG, Lee NH. Compounds with elastase inhibition and free radical scavenging activities from Callistemon lanceolatus. J Med Plants Res. 2009;3(11):914–20.
10. Karim AA, Azlan A, Ismail A, Hashim P, Abd Gani SS, Zainudin BH, et al. and Tyrosinase Inhibitory Activities of Cocoa Pod Extract. BMC Complement Altern Med. 2014;14(381):1–13.
11. Qonita F. Pemanfaatan Ekstrak Buah Strawberry (Fragaria X Ananassa (Duchesne Ex Weston)) Sebagai Bahan Kosmetik: Uji Aktivitas Antiaging dan Antibakteri Secara In Vitro. Yogyakarta: Faculty of Pharmacy, Gadjah Mada University; 2017.
12. Syamsu N. Pemanfaatan Ekstrak Etanolik dan Etil Asetat Daging Buah dan Kulit Buah Langsat (Lansium Domesticum (Corr)) Sebagai Bahan Aktif Kosmetik: Uji Aktivitas Antiaging dan Anti Jerawat Secara In Vitro. Yogyakarta: Faculty of Pharmacy, Gadjah Mada University; 2017.
13. Zulbayu L ode. Uji Aktivitas Antiaging dan Antibakteri Ekstrak Etanol dan Etil Asetat Kulit Buah Jeruk Bali (Citrus maxima L.) secara In Vitro. Yogyakarta: Faculty of Pharmacy, Gadjah Mada University; 2018.
14. Khairunnisa N. Formulasi Gel dan Krim Penangkap Radikal 2,2-Difenil-1-Pikrilhidrazil dari Kombinasi Ekstrak Buah Strawberry, Ekstrak Kulit Buah Jeruk Bali dan Ekstrak Buah Langsat serta Uji Stabilitasnya. Yogyakarta: Faculty of Pharmacy, Gadjah Mada University; 2018.
15. Prabaswari N. Formulasi serta Uji Stabilitas Kombinasi Ekstrak Buah Strawberry, Buah Langsat, dan Kulit Buah Jeruk Bali dalam Krim-Penangkap Radikal DPPH (2,2, Difenil-1-Pikrilhidrazin),. Yogyakarta: Faculty of Pharmacy, Gadjah Mada University; 2018.
16. Talegaonkar S, Tariq M, Alabood RM. An Official Publication of Association of Pharmacy Professionals D Esign and Development of O/W nanoemulsion. Bull Pharm Res. 2011;1(3):18–30.
17. Ya Luo, Hao-ru Tang *, Xiao-rong Wang, Yong Zhang ZL. Antioxidant properties and involved antioxidant compounds of strawberry fruit at different maturity stages. J Food, Agric Environ. 2011;9(1):166–70.
18. Lukitaningsih E. and Holzgrabe U., Bioactive Compounds in Bengkoang (Pachyrhizus erosus) AS Antioxidant and Tyrosinase Inhibiting Agents. Indones J Pharm. 2014;25(2):68.
19. Wong SK, Lim YY, Chan EWC. Evaluation of antioxidant, anti-tyrosinase and antibacterrial activities of selected Hibiscus species. Ethobotanical Leafl. 2010;14(June):781–96.
20. Biswas M, Haldar PK, Ghosh AK. Antioxidant and free-radical-scavenging effects of fruits of Dregea volubilis. J Nat Sci Biol Med. 2010;1(1):29–34.
21. Ndlovu G, Fouche G, Tselanyane M, Cordier W, Steenkamp V. In vitro determination of the anti-aging potential of four southern African medicinal plants. BMC Complement Altern Med. 2013;13.
22. Klein T, Bischoff R. Physiology and pathophysiology of matrix metalloproteases. Amino Acids. 2011;41(2):271–90.
23. Lukitaningsih E, Holzgrabe U. A New Compound (8,9) -Furanyl-Pterocarpan-3-Ol Used for Standardization of Bengkuang (Pachyrhizus erosus) Extract as Sunscreen and Skin Whitening Agent. Indones J Cancer Chemoprevention. 2019;10(2):60.
24. Widasri M. Topical Microemulsion Antiaging Formulation of Strawberry Fruit (Fragaria x ananassa Duchesne), Langsat Fruit (Lansium domesticum Correa.), Pomelo Peel (Citrus maxima (Burm.) Merr.) Extracts Mixture. Yogyakarta: Faculty of Pharmacy, Gadjah Mada University; 2018.
25. Hermadi Saputro A. Topical Microemulgel Antiaging Formulation of Strawberry Fruit (Fragaria x ananassa Duchesne), Langsat Fruit (Lansium domesticum Correa.), Pomelo Peel (Citrus maxima (Burm.) Merr.) Extracts Mixture. Yogyakarta: Faculty of Pharmacy, Gadjah Mada University; 2018.
26. Sipahli S, Mohanlall V, Mellem JJ. Stability and degradation kinetics of crude anthocyanin extracts from H. sabdariffa. Food Sci Technol. 2017;37(2):209–15.
Copyright (c) 2021 Indonesian Journal of Chemometrics and Pharmaceutical Analysis
This work is licensed under a Creative Commons Attribution 4.0 International License.
Author(s) Rights
As a journal Author, you have rights for a large range of uses of your article, including use by your employing institute or company. These Author rights can be exercised without the need to obtain specific permission. Authors publishing in IJCPA journals have wide rights to use their works for teaching and scholarly purposes without needing to seek permission, including: use for classroom teaching by Author or Author's institutionand presentation at a meeting or conference and distributing copies to attendees; use for internal training by author's company; distribution to colleagues for their reseearch use; use in a subsequent compilation of the author's works; inclusion in a thesis or dissertation; reuse of portions or extracts from the article in other works (with full acknowledgement of final article); preparation of derivative works (other than commercial purposes) (with full acknowledgement of final article); voluntary posting on open web sites operated by author or author’s institution for scholarly purposes (should follow CC by SA License).
Authors can copy and redistribute the material in any medium or format, as well as remix, transform, and build upon the material for any purpose, even commercially, but they must give appropriate credit (cite to the article or content), provide a link to the license, and indicate if changes were made. If you remix, transform, or build upon the material, you must redistribute your contributions under the same license as the original.