β-Sitosterol: The Isolated Compound from n-Hexane Fraction of Baccaurea racemosa (Reinw. ex Blume) Müll. Arg. Pulp and Its Antioxidant Activity

  • Lina Permatasari Department Of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
  • Sugeng Riyanto Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, Indonesia
  • Abdul Rohman Center of Excellence, Institute for Halal Industry and System, Universitas Gadjah Mada, Yogyakarta, Indonesia
Keywords: n-Hexane fraction; Antioxidant activity; Baccaurea racemosa pulp; β-sitosterol; Isolation

Abstract

Baccaurea racemosa is one of the most widespread plants in Indonesia. The constituent compound at Baccaurea racemosa influenced its biological activity. However, the isolation of compounds from the n-hexane fraction of B. racemosa pulp has never been reported. This study aimed to isolate and spectroscopic analysis of compound from n-hexane fraction of Baccaurea racemosa pulp and its antioxidant activity. The fraction of n-hexane was isolated using the preparative thin-layer chromatography (PLC) method. The purified compound was identified its structure compound using the spectroscopic method. Then the antioxidant activity was determined using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. UV-Vis spectrum of isolated compounds revealed the absence of conjugated double bonds. Furthermore, the infrared spectrum showed OH, C-H aliphatic, C-O, C-H vanillic, and C=C groups. The molecular ion of the isolated compound was 414 g/mol. The NMR spectrum showed several peaks at various chemical shifts. Based on the spectral analysis, the isolated compound was indicated as β-sitosterol. This compound has antioxidant activity with IC50 > 2.4155 mM.

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Published
2021-12-29
How to Cite
Permatasari, L., Riyanto, S., & Rohman, A. (2021). β-Sitosterol: The Isolated Compound from n-Hexane Fraction of Baccaurea racemosa (Reinw. ex Blume) Müll. Arg. Pulp and Its Antioxidant Activity. Indonesian Journal of Chemometrics and Pharmaceutical Analysis, 1(3), 156-163. https://doi.org/10.22146/ijcpa.3693
Section
Original Articles