Halal Authentication of Toothpaste with FTIR Combine with Chemometrics

  • Wiranti Sri Rahayu Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Banyumas 53183, Indonesia
  • Pri Iswati Utami Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Banyumas 53183, Indonesia
  • Bella Mahardika Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Banyumas 53183, Indonesia
Keywords: gelatine, toothpaste, FTIR


Gelatin is a raw material used in pharmaceutical and cosmetic preparations, one of which is toothpaste. Some of dental raw materials are imported from another country, and might contain porcine gelatin, Authentication of gelatin in toothpaste products is crucial due to  religious and health reasons. The purpose of this study was to detect porcine gelatin in toothpaste raw materials using ATR-FTIR. Five samples of toothpaste were purchased from local market and detection was done using ATR-FTIR. The spectrum from each sample was compared against standard bovine and porcine gelatin. Experimental toothpaste containing bovine and porcine gelatin at concentrations of 5, 10, 15 and 20% were also prepared for quantification analysis. FTIR combine with chemometrics can be utilized as a reliable tool to detect gelatin in toothpaste and other pharmaceuticals.


1. Hunter, M., 2012. The emerging halal cosmetic. Personal Care 3, 37-41.
2. Mubarok, K.F., and Imam, M., K., 2020. Halal Industry in Indonesia; Challenges and Opportunities. Journal of Digital Marketing and Halal Industry, 2(1), 55-63.
3. Ramadhani, A., Wijaya, S., Mardlianah A., Adiatman, M., Setiawati, F., Gunawan, H.A., and Maharani, D.A., 2020. Fluoride content and labelling of toothpastes marketed in Indonsia. Journal of Stomatology, 73(4), 193-199.
4. Irfanita, N., Jaswir, 1., Mirghani, M. E. S., Sukmasari, M. S., Ardini, Y. D., and Lestari, W. 2017. Rapid detection of Gelatin in Dental Materials using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR). Journal of Physics : Conf. Series, 884 (2017) 012090.
5. Mariod, A., A., and Adam, H., F., 2013. Review: Gelatin, Source, Extraction and Industrial Application. Acta Sci. Pol., Technol. Aliment, 12(2), 135-147.
6. Demirhan, Y., Ulca, P., and Senyuva, H.Z., Detection of Porcine DNA in Gelatine and Gelatine containing Processed Food Products-Halal/Kosher Authentification, Meat Science. 2012. 90: 686-689
7. Zilhadia, Yahdiana H, Irwandi J, &Effionora A. 2018. Characterization and functional properties of gelatin extracted from goatskin. International Food Research Journal, 25(1), 275-281.
8. Che Man et al. 2010. Fourier Transformed Infrared (FTIR) Spectroscopy: Development, Techniques, And Application In The Analyses of Fats and Oils. Oliver J Ress (Ed.) NeW York : Nova Science Publishers
9. Hashim., D. M., Che Man, Y. B., Norakasha, R., Shuhaimi, M., Shariza, Z. A. 2010. Potential Use of Fourier Trasform Infrared Spectroscopy for differentiation of bovine and porcine gelatins. Food Chemistry. 118 : 856 – 860.
10. Hermanto, S, Sumarlin, L. O. and Fatimah, W. 2013. Differentiation of bovine and porcine gelatine based on spectroscopic and electrophoretic analysis. J. Food. Pharm. Sci. 1 : 68-73.
11. Miller, J. N., & Miller, J.C. 2010. Statistics and Chemometrics for Analytical Chemistry. (6 th ed) Harlow, Essex : Pearson.
12. Hasan, N., Tahir, A., and Zain, N. M., 2018. Chemical and Chemometric Methods for Halal Authentication of Gelatin: An Overview. JFDS 83 (12): 2903-2911
How to Cite
Sri Rahayu, W., Iswati Utami, P., & Mahardika, B. (2021). Halal Authentication of Toothpaste with FTIR Combine with Chemometrics. Indonesian Journal of Chemometrics and Pharmaceutical Analysis, 1(2), 78-82. https://doi.org/10.22146/ijcpa.1744
Original Articles