* Corresponding Author
Effect of Different Concentrations and Combinations of Benzyl Aminopurine and Indole-3-Butyric Acid on Micropropagation of Vanilla Planifolia
https://doi.org/10.22146/jtbb.90734 Sokhai Khun(1), Chenda Heng(2), Sothea Rien(3), Sinet Rien(4), Pao Srean(5*)
(1) AgroBio4Cam – ABC, Battambang 020801, Cambodia
(2) AgroBio4Cam – ABC, Battambang 020801, Cambodia
(3) Faculty of Agriculture and Food Processing, National University of Battambang, Battambang 021402, Cambodia
(4) AgroBio4Cam – ABC, Battambang 020801, Cambodia
(5) AgroBio4Cam – ABC, Battambang 020801, Cambodia; Faculty of Agriculture and Food Processing, National University of Battambang, Battambang 021402, Cambodia
(*) Corresponding Author
Abstract
Micropropagation of explants in vitro has potency to address propagule demands for promoting large-scale vanilla production. Plant growth regulators (i.e., cytokinin, auxin) are necessary for the plant micropropagation success. Objective of this study is to determine the shoot multiplication and root development of Vanilla planifolia under the influence of different concentrations and combinations of BA and IBA for micropropagation. Sixty stem nodal segments of Vanilla planifolia were cultured on MS medium supplemented with IBA (0, 0.5, or 2.0 mg/L) and combined with BA (0, 0.5, 1.0, or 2.0 mg/L). Shoot multiplication and root induction were measured after 60 days of culture. The results show that the MS medium with 1 mg/L IBA hindered shoot growth, while the medium containing 1 mg/L BA yielded the highest number or weight of shoots per explant. For the root development, supplementing the medium with 0.5 mg/L or 1 mg/L IBA improved root length or number of roots per explant, respectively. This research establishes a valuable approach for vanilla micropropagation by utilising low concentrations of plant growth regulators and a rapid protocol. This paves the way for significant advancements in large-scale commercial production.
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Abebe, Z. et al., 2009. Efficient in vitro multiplication protocol for Vanilla planifolia using nodal explants in Ethiopia. African Journal of Biotechnology, 8(24), pp.6817-6821.
Anuradha, K., Shyamala, B.N. & Naidu, M.M., 2013. Vanilla-its science of cultivation, curing, chemistry, and nutraceutical properties. Critical Reviews in Food Science and Nutrition, 53(12), pp.1250-1276. doi: 10.1080/10408398.2011.563879.
Arya, S.S. et al., 2021. Vanillin: A review on the therapeutic prospects of a popular flavouring molecule. Advances in Traditional Medicine, 21, pp.1–17. doi: 10.1007/s13596-020-00531-w.
Ayele, Y., Tefera, W. & Bantte, K., 2017. Enhanced Protocol Development for in vitro Multiplication and Rooting of Vanilla (Vanilla planifolia Andr.) Clone (Van. 2/05). Biotechnology Journal International, 18(3), pp.1-11.
Bhatla, S.C. & Lal, M.A., 2023. Plant Growth Regulators: An Overview. In Plant Physiology, Development and Metabolism. Singapore: Springer, pp.391–398. doi: 10.1007/978-981-99-5736-1_14
Baqueiro-Peña, I. & Guerrero-Beltrán, J.Á., 2017. Vanilla (Vanilla planifolia Andr.), its residues and other industrial by-products for recovering high value flavor molecules: A review. Journal of Applied Research on Medicinal and Aromatic Plants, 6, pp.1-9. doi: 10.1016/j.jarmap.2016.10.003.
Bello-Bello, J.J. et al., 2016. Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews). African Journal of Biotechnology, 15(8), pp.272-277. doi: 10.5897/AJB2015.14662.
Cameron, K., 2011. Vanilla Orchids: Natural History and Cultivation, Portland: Timber Press.
de Oliveira, S.O.D. et al., 2013. A new procedure for in vitro propagation of vanilla (Vanilla planifolia) using a double-phase culture system. Scientia Horticulturae, 161, pp.204-209. doi: 10.1016/j.scienta.2013.06.039.
DMNP, 2023, ‘Vanilla Global Market Report – June 2023’, in De Monchy Natural Products, viewed 15 November 2023, from https://monchynaturalproducts.com/news/vanilla-global-market-report-dmnp.
Erawati, D.N. et al., 2020. Micropropagation of vanilla (Vanilla planifolia Andrews) with modification of cytokinins. IOP conference series: Earth and environmental science, 411(1), 012009.
Erawati, D.N. et al., 2021. Shoots multiplication of vanilla (Vanilla planifolia) with benzyl amino purine and kinetin modification. IOP conference series: Earth and environmental science, 672, 012007. doi: 10.1088/1755-1315/672/1/012007.
Gamborg, O.L., Miller, R.A. & Ojima, K., 1968. Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50, pp.151–158. doi: 10.1016/0014-4827(68)90403-5.
George, P.S. & Ravishankar, G.A., 1997. In vitro multiplication of Vanilla planifolia using axillary bud explants. Plant Cell Reports, 16, pp.490-494. doi: 10.1007/BF01092772
Giridhar, P. & Ravishankar, G.A., 2004. Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk. Indian Journal of Biotechnology, 3, pp.113 – 118.
Izzati, K.H.F.L. et al., 2013. A simple and efficient protocol for the mass propagation of Vanilla planifolia. American Journal of Plant Sciences, 4(09), 1685.
Janarthanam, B. & Seshadri, S., 2008. Plantlet regeneration from leaf derived callus of Vanilla planifolia Andr. In vitro Cellular Developmental Biology‐Plant, 44, pp.84‐89. doi: 10.1007/s11627-008-9123-4.
Kalimuthu, K., Senthilkumar, R. & Murugalatha, N., 2006. Regeneration and mass multiplication of Vanilla planifolia Andr. – a tropical orchid. Current Science, 91(10), pp.1401-1403.
Kunwanlop, W. et al., 2018. Effect of plant growth regulators on micropropagation of Vanilla aphylla and Vanilla planifolia sp. Variegate. International Journal of Agricultural Technology, 14(7), pp.1357 – 1364.
Kruskal, W.H. & Wallis, W.A., 1952. Use of ranks in one-criterion variance analysis. Journal of the American Statistical Association, 47(260), pp.583-621. doi: 10.2307/2280779
McGregor, A., 2004. Diversification into High-Value Export Products: Case Study of the Papua New Guinea Vanilla Industry. Food and Agriculture Organization of the United Nations.
Murashige, T. & Skoog, F., 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), pp.473-497. doi: 10.1111/j.1399-3054.1962.tb08052.x
Philip, V.J. & Nainar, S.A.Z., 1986. Clonal propagation of Vanilla planifolia (Salisb.) Ames using tissue culture. Journal of Plant Physiology, 122(3), pp.211-215. doi: 10.1016/S0176-1617(86)80119-5.
Prabaninggar, R.A., Sasmita, E.R. & Wahyurini, E., 2021. In vitro micro-cutting of vanilla (Vanilla Planifolia Andrews.) in different NAA and BAP. Journal Techno, 7(1), pp.027-036.
R Core Team, 2020, ‘R: A language and environment for statistical computing’, in RProject, viewed from https://www.Rproject.org
Ramachandra Rao, S. & Ravishankar, G., 2002. Vanilla flavour: production by conventional and biotechnological routes. Journal of the Science of Food and Agriculture, 80, pp.289–304. doi: 10.1002/1097-0010(200002)80:3%3C289::AID-JSFA543%3E3.0.CO;2-2.
RBG Kew, 2023, ‘Vanilla planifolia: Vanilla’, in Royal Botanic Gardens, Kew, viewed 15 November 2023, from https://www.kew.org/plants/vanilla
Sharma, R.U.B.Y. & Bora, S.U.N.I.L., 2017. Influence of explants type and plant growth regulators on in vitro multiple shoots regeneration of Vanilla planifolia. International Jouranl of Agricultural Science and Research, 7(2), pp. 189-196.
Sidek, N. et al., 2018. The effect of different nutrient media on in vitro shoot and root proliferation of Vanilla planifolia Jacks. ex Andrews. African Journal of Biotechnology, 17(39), pp.1241-1246. doi: 10.5897/AJB2018.16610.
Sinha, A.K., Sharma, U.K. & Sharma, N., 2008. A comprehensive review on vanilla flavor: Extraction, isolation and quantification of vanillin and others constituents. International Journal of Food Sciences and Nutrition, 59(4), pp.299-326. doi: 10.1080/09687630701539350.
Srean, P., Mosaleeyanon, K. & Kirdmanee, C., 2011. Effects of photosynthetic photon flux and supporting material on the growth and development of Dendrobium sp. plantlets in vitro. Cambodian Journal of Agriculture, 10(1-2), pp.1–4.
Tan, B.C., Chin, C.F. & Alderson, P., 2011. An improved plant regeneration of Vanilla planifolia Andrews. Plant Tissue Culture and Biotechnology, 21(1), pp.27-33.
Tukey, J., 1949. Comparing Individual Means in the Analysis of Variance. Biometrics, 5(2), pp.99-114. doi: 10.2307/3001913.
Wickham, H., 2011. ggplot2. WIREs Computational Statistics, 3(2), pp.180-185. doi: 10.1002/wics.147
Zuraida, et al., 2013. A simple and efficient protocol for the mass propagation of Vanilla planifolia. American Journal of Plant Sciences, 4(9), pp.1685-1692. doi: 10.4236/ajps.2013.49205.
DOI: https://doi.org/10.22146/jtbb.90734
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