* Corresponding Author
Therapeutic Effects of BRC Functional Food from Indonesian Black Rice on Body Weight and Haematological Parameters in Obese Rats
https://doi.org/10.22146/jtbb.85847 Fajar Sofyantoro(1), Adi Mazdi Syam(2), Baik Aisyah Adania(3), Muhammad Fikri Almunawar(4), Nurlita Putri Bela Nasution(5), Rheina Faticha Asyamsa Hidayat(6), Made Bagus Auriva Mataram(7), Chesa Ekani Maharesi(8), Septika Nurhidayah(9), Yekti Asih Purwestri(10), Ardaning Nuriliani(11), Lisna Hidayati(12), Rarastoeti Pratiwi(13*)
(1) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(2) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(3) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(4) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(5) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(6) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(7) Pre-clinical and Experimental Animal Unit (Unit IV), Integrated Research and Testing Laboratory, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
(8) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(9) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(10) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281; Research Centre for Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(11) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(12) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(13) Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281; Research Centre for Biotechnology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(*) Corresponding Author
Abstract
Obesity increases the risk of various diseases. Black rice, renowned for its high anthocyanin content, is considered a potential functional food for preventing metabolic disorders. The current study investigated the effects of black rice crunch (BRC) on body weight and haematological profiles in obese rats. Rats were fed with high-fat diet to induce obesity and supplemented with different concentrations of BRC for 4 and 8 weeks. The results showed that high-fat diet effectively induced obesity, as evidenced by significant increase in body weight. Importantly, 75% BRC supplementation resulted in significant weight reduction in obese rats. Further analysis revealed an increase in erythrocyte numbers in obese groups supplemented with 75% BRC, but no significant changes in haemoglobin concentration or haematocrit percentage. Further investigation showed that 75% BRC led to a decrease in mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), and mean corpuscular volume (MCV), potentially affecting the size and concentration of haemoglobin within erythrocytes. The total leucocytes count increased with the high-fat diet, while BRC supplementation alone did not have significant impact. Lymphocyte percentage remained stable across the groups, indicating minimal influence of the dietary interventions. Neutrophil percentage varied initially but was not specific to BRC or the high-fat diet. Platelet count and distribution width were not significantly influenced, but mean platelet volume (MPV) increased after 8 weeks of BRC treatment, suggesting larger platelet sizes associated with obesity. Overall, the study provides important insights into the effects of BRC supplementation on body weight and haematological parameters related to obesity.
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Aktas, G. et al., 2018. Mean Platelet Volume (MPV) as an inflammatory marker in type 2 diabetes mellitus and obesity. Bali Medical Journal, 7(3). doi: 10.15562/bmj.v7i3.806.
An, J.S. et al., 2016. In vitro potential of phenolic phytochemicals from black rice on starch digestibility and rheological behaviors. Journal of Cereal Science, 70, pp.214–220. doi: 10.1016/j.jcs.2016.06.010.
Barrachina, M.N. et al., 2020. Analysis of platelets from a diet-induced obesity rat model: elucidating platelet dysfunction in obesity. Scientific Reports, 10(1), p.13104. doi: 10.1038/s41598-020-70162-3.
Brandl, E.J. et al., 2012. Association study of polymorphisms in leptin and leptin receptor genes with antipsychotic-induced body weight gain. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 38(2), pp.134–141. doi: 10.1016/j.pnpbp.2012.03.001.
Buettner, R., Schölmerich, J. & Bollheimer, L.C., 2007. High-fat Diets: Modeling the Metabolic Disorders of Human Obesity in Rodents. Obesity, 15(4), pp.798–808. doi: 10.1038/oby.2007.608.
Chasanah, S.N. & Pratiwi, R., 2019. Kadar Serum Glutamic Pyruvate Transaminase Darah Tikus (Rattus norvegicus Berkenhout, 1769) Hiperlipidemia dengan Asupan Pelet Nasi dan Bekatul Beras Hitam Padi (Oryza sativa L.) “Cempo Ireng.” Cendekia Eksakta, 4(1), pp.33–38. doi: 10.3194/ce.v4i1.2672.
Cox, D., Kerrigan, S.W. & Watson, S.P., 2011. Platelets and the innate immune system: mechanisms of bacterial‐induced platelet activation. Journal of Thrombosis and Haemostasis, 9(6), pp.1097–1107. doi: 10.1111/j.1538-7836.2011.04264.x.
Després, J.-P., 2012. Body Fat Distribution and Risk of Cardiovascular Disease: An Update. Circulation, 126(10), pp.1301–1313. doi: 10.1161/CIRCULATIONAHA.111.067264.
Després, J.-P. & Lemieux, I., 2006. Abdominal obesity and metabolic syndrome. Nature, 444(7121), pp.881–887. doi: 10.1038/nature05488.
Fabroni, S. et al., 2016. Screening of the anthocyanin profile and in vitro pancreatic lipase inhibition by anthocyanin-containing extracts of fruits, vegetables, legumes and cereals: Anthocyanin-containing extracts as inhibitors of pancreatic lipase. Journal of the Science of Food and Agriculture, 96(14), pp.4713–4723. doi: 10.1002/jsfa.7708.
Hariri, N., Gougeon, R. & Thibault, L., 2010. A highly saturated fat-rich diet is more obesogenic than diets with lower saturated fat content. Nutrition Research, 30(9), pp.632–643. doi: 10.1016/j.nutres.2010.09.003.
de Kort, M. et al., 2020. Historical control data for hematology parameters obtained from toxicity studies performed on different Wistar rat strains: Acceptable value ranges, definition of severity degrees, and vehicle effects. Toxicology Research and Application, 4, p.239784732093148. doi: 10.1177/2397847320931484.
Kristamtini, T. et al., 2012. Morphological of genetic relationships among black rice landraces from Yogyakarta and surrounding areas Kristamtini, T., Basunanda, P., Murti, R. H., Supriyanta, S. W., & Sutarno, W. ARPN Journal of Agricultural and Biological Science, 7(12), pp.982–989.
Lim, W.-C. et al., 2016. Germinated Waxy Black Rice Suppresses Weight Gain in High-Fat Diet-Induced Obese Mice. Journal of Medicinal Food, 19(4), pp.410–417. doi: 10.1089/jmf.2015.3590.
Lin, X. & Li, H., 2021. Obesity: Epidemiology, Pathophysiology, and Therapeutics. Frontiers in Endocrinology, 12, p.706978. doi: 10.3389/fendo.2021.706978.
Monteomo, G., Kamagate, A. & Yapo, A., 2018. Effects of metabolic syndrome on blood cells to Wistar rats. Journal of Diabetes, Metabolic Disorders & Control, 5(6). doi: 10.15406/jdmdc.2018.05.00170.
Mulyati et al., 2019. Potensi Nutriulva sebagai Suplemen Hematologis (Laporan Penelitian Kolaborasi Dosen dan Mahasiswa), Yogyakarta, Indonesia: Faculty of Biology, Universitas Gadjah Mada.
Nasution, N.P.B. & Pratiwi, R., 2023. Effects of the Functional Food Product “Black Rice Crunch” on Leukocyte and Thrombocyte Profiles of Obese Rats (Rattus norvegicus Berkenhout, 1796). Yogyakarta, Indonesia: Universitas Gadjah Mada.
Park, Y.M. et al., 2020. Immunostimulatory Activity of Black Rice Bran in Cyclophosphamide-Induced Immunosuppressed Rats. Natural Product Communications, 15(7), p.1934578X2093491. doi: 10.1177/1934578X20934919.
Pratiwi, R. et al., 2019. Active Fractions of Black Rice Bran cv Cempo Ireng Inducing Apoptosis and S-phase Cell Cycle Arrest in T47D Breast Cancer Cells. Journal of Mathematical and Fundamental Sciences, 51(1), pp.47–59. doi: 10.5614/j.math.fund.sci.2019.51.1.4.
Pratiwi, R. et al., 2016. Black Rice Bran Extracts and Fractions Containing Cyanidin 3-glucoside and Peonidin 3-glucoside Induce Apoptosis in Human Cervical Cancer Cells. Indonesian Journal of Biotechnology, 20(1), p.69. doi: 10.22146/ijbiotech.15271.
Purwestri, Y.A. et al., 2022. Makanan Fungsional Berbahan Dasar Beras Hitam dan Proses Pembuatannya.
Ren, D. et al., 2019. Hypolipidemic effects of fucoidan fractions from Saccharina sculpera (Laminariales, Phaeophyceae). International Journal of Biological Macromolecules, 140, pp.188–195. doi: 10.1016/j.ijbiomac.2019.08.002.
Romieu, I. et al., 2017. Energy balance and obesity: what are the main drivers? Cancer Causes & Control, 28(3), pp.247–258. doi: 10.1007/s10552-017-0869-z.
Rukmana, R.M. et al., 2017. The Effect of Ethanolic Extract of Black and White Rice Bran (Oryza sativa L.) on Cancer Cells. Indonesian Journal of Biotechnology, 21(1), p.63. doi: 10.22146/ijbiotech.26814.
Sa’adah, N.N. & Pratiwi, R., 2016. Profil Lipid dan Indeks Aterogenik Tikus Putih (Rattus norvegicus Berkenhout, 1769) Hiperlipidemia dengan Asupan Pelet Nasi dan Bekatul Beras Hitam (Oryza sativa L.) “Cempo Ireng.” In Seminar Nasional Biodiversitas VI. Seminar Nasional Biodiversitas VI. Surabaya, Indonesia, pp. 1–10.
Said, N. & Abiola, O., 2014. Haematological profile shows that Inbred Sprague Dawley rats have exceptional promise for use in biomedical and pharmacological studie. Asian Journal of Biomedical and Pharmaceutical Sciences, 4(37), pp.33–37. doi: 10.15272/ajbps.v4i37.597.
Shabbir, F., Hussain, M.M. & Rajput, T.A., 2015. Obesity: Effect of High Fat Diet Followed by Atorvastatin Administration on Serum Interleukin-6, White Blood Cell and Platelet Count in Male and Female Sprague Dawley Rats. The Professional Medical Journal, 22(06), pp.683–389. doi: 10.29309/TPMJ/2015.22.06.1231.
Sharp, P. & Villano, J.S., 2012. The Laboratory Rat 2nd Edition., Boca Raton: CRC Press. Available at: https://doi.org/10.1201/b13862.
Syam, A.M. & Pratiwi, R., 2023. Effects of Functional Food Product “Black Rice Crunch” on Body Weight and Red Blood Cell Profile of Obese Rats (Rattus norvegicus Berkenhout, 1769). Yogyakarta, Indonesia: Universitas Gadjah Mada.
Tsalissavrina, I., Wahono, D. & Handayani, D., 2013. Pengaruh Pemberian Diet Tinggi Karbohidrat dibandingkan Diet Tinggi Lemak terhadap Kadar Trigliserida dan HDL Darah pada Rattus novergicus galur wistar. Jurnal Kedokteran Brawijaya, 22(2), pp.80–89. doi: 10.21776/ub.jkb.2006.022.02.5.
Von Diemen, V., Trindade, E.N. & Trindade, M.R.M., 2006. Experimental model to induce obesity in rats. Acta Cirurgica Brasileira, 21(6), pp.425–429. doi: 10.1590/S0102-86502006000600013.
Zhang, M.W. et al., 2010. Phenolic Profiles and Antioxidant Activity of Black Rice Bran of Different Commercially Available Varieties. Journal of Agricultural and Food Chemistry, 58(13), pp.7580–7587. doi: 10.1021/jf1007665.
DOI: https://doi.org/10.22146/jtbb.85847
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