Diversity of  Fusarium  Endophytes Isolated from Wild Bananas in Pandenglang, Indonesia

Nani Maryani; Siti Yulianti; Indriati Ramadhani; Rida Oktorida Khastini; Suroso Mukti Leksono

doi:10.22146/jtbb.76897

Diversity of Fusarium Endophytes Isolated from Wild Bananas in Pandenglang, Indonesia

https://doi.org/10.22146/jtbb.76897

Nani Maryani^(1*), Siti Yulianti⁽²⁾, Indriati Ramadhani⁽³⁾, Rida Oktorida Khastini⁽⁴⁾, Suroso Mukti Leksono⁽⁵⁾

(1) Department of Biology Education, Universitas Sultan Ageng Tirtayasa (UNTIRTA), Jl. Raya Ciwaru No.25, Kota Serang, Banten 42124; Center of Excellence for Local Food Innovation, Jl. Raya Palka Km.3 Sindangsari, Kec. Pabuaran, Serang, Banten 42163
(2) Department of Biology Education, Universitas Sultan Ageng Tirtayasa (UNTIRTA), Jl. Raya Ciwaru No.25, Kota Serang, Banten 42124
(3) Research Center for Biology, National Research and Innovation Agency (BRIN), Cibinong Bogor
(4) Department of Biology Education, Universitas Sultan Ageng Tirtayasa (UNTIRTA), Jl. Raya Ciwaru No.25, Kota Serang, Banten 42124
(5) Department of Science Education, Universitas Sultan Ageng Tirtayasa (UNTIRTA), Jl. Raya Ciwaru No.25, Kota Serang, Banten 42124
(*) Corresponding Author

Abstract

A group of Fusarium spp., in the Fusarium oxysporum species complex is known as pathogens on bananas, i.e., Fusarium wilt or Panama Disease. However, many Fusarium spp. are also known to be endophytes inside healthy banana plants and have been less explored and investigated. Fusarium endophytes have been demonstrated to be effective against the Fusarium pathogen that causes wilting in some crops such as tomatoes and watermelon. Thus, we explored endophytes Fusarium from local bananas in Pandenglang Banten for further use as biocontrol of Fusarium wilt. Four wild banana accessions were identified, from which 9 Fusarium isolates recovered from its pseudostems asymptomatic plants. All isolates were characterized based on their morphological characters and sequence of the Internal Transcribed Spacer (ITS) gene. These isolates belong to four complexes of Fusarium i.e. Fusarium equiseti species complex, Fusarium oxysporum species complex, Fusarium sambucinum species complex, and Fusarium solani species complex (currently described as Neocosmospora). Further study on molecular characterization of these isolates using specific genes and their potential antagonists of pathogens still needs to be discovered for other use as a biocontrol against Fusarium wilt.

Keywords

Banten, endophyte, fungi, Fusarium, Musa

Full Text:

PDF

References

Ahmad, F., Maryani, N. & Poerba, Y.S., 2020. Genetic mapping of Fusarium wilt resistance in a wild 1 banana Musa acuminata ssp. malaccensis accession. TheorApplyGenet., 133, pp.3409-3418. doi: 10.1007/s00122-020-03677-y.

Ahmad, F., 2021. Genetics and diversity of Indonesian Bananas. Wageningen University and Research (WUR): The Netherlands.

Aimé, S. et al., 2013. The endophytic strain Fusarium oxysporum Fo47: A good candidate for priming the defense responses in tomato roots. Mol Plant-Microbe Interact., 26 (8), pp.918–926. Doi: 10.1094/MPMI-12-12-0290-R.

Constantine, M.E., Vlieger, B.V. & Takken, F.L.W., 2020. Diminished Pathogen and Enhanced Endophyte Colonization upon CoInoculation of Endophytic and Pathogenic Fusarium Strains. Microorganisms, 8(4), pp.544. doi: 10.3390/microorganisms8040544.

Dale, J., James, A. & Paul, J.Y., 2017. Transgenic Cavendish bananas with resistance to Fusarium wilt tropical race 4. Nat Commun., 8, Article number: 1496. doi: 10.1038/s41467-017-01670-6

Hidayat, I. & Ramadhani, I., 2019. Phylogenetic study of Curvularia on Sorgum from Indonesia Based on ITS rDNA Sequence. Jurnal Mikologi Indonesia, 3(2), pp.118-124.

Jia, M. et al., 2016. A Friendly Relationship between Endophytic Fungi and Medicinal Plants: A Systematic Review. Front Microbiol, 7, pp.906. doi: 10.3389/fmicb.2016.00906.

Kema, G.H.J., et al. 2021. Editorial: Fusarium Wilt of Banana, a Recurring Threat to Global Banana Production. Front. Plant Sci., 11, 628888. doi: 10.3389/fpls.2020.628888

Leslie, J.F. & Summerell, B.A., 2006. The Fusarium laboratory manual, UK: Blackwell Publishing Ltd.

Maryani, N., 2018. A complex relationship: Banana and Fusarium wilt in Indonesia. Wegeningen University and Research (WUR): The Netherlands.

Maryani, N. et al., 2019. New Endemic Fusarium species hitch-hiking with Pathogenic Fusarium Strains Causing Panama disease in small-holder Banana Plots in Indonesia. Persoonia-Molecular Phylogeny and Evolution of Fungi, 43, pp.48-69. doi: 10.3767/persoonia.2019.43.02

Nasution, R.E, 1990. A taxonomic Study of the Species Musa acuminata Colla with its intraspecific taxa in Indonesia. Tokyo University of Agriculture, Japan.

Núñez-Trujillo, G. et al., 2013. Survey of Banana endophytic fungi isolated in artificial culture media from an applied viewpoint. J Horti Fores Biotech, 17, pp.22-25.

Nuraini, F.R., Setyaningsih, R. & Susiolowati, A., 2017. Screening and Characterization of Endophytic Fungi as Antagonistic Agents Toward Fusarium oxysporum on Eggplant (Solanum melongena). Biodiversitas, 18(4), pp.1377-1384. doi: 10.13057/biodiv/d180413

O’donneal, K. et al., 2008. Molecular Phylogenetic Diversity, Multilocus Haplotype Nomenclature, and in vitro Antifungal Resistance within the Fusarium solani species Complex. J Clin Microbiol, 46(8), pp.2477-2490. doi: 10.1128/JCM.02371-07.

Ordonez, N. et al., 2015. Worse comes to worst: banana and Panama disease: when plant and pathogen clones meet. PLoS Pathogen, 11(11), e1100519. doi: 10.1371/journal.ppat.1005197.

Ploetz, R.C., Haynezm, J., & Vazquez, A., 1999. Responses of New Banana Accessions in South Florida to Panama Disease. Crop Prot, 18, pp.445-449. doi: 10.1016/S0261-2194(99)00043-5

Ploetz, R.C., 2006. Panama disease, an old nemesisrears its ugly head: part2, the Cavendish era and beyond. Plant Health Progress, 7(1), pp.1-17. doi: 10.1094/PHP-2006-0308-01-RV

Poerba, Y.S., Witjaksono, D. & Martanti, 2018. Katalog Pisang: Koleksi Kebun Plasma Nutfah Pisang Pusat Penelitian Biologi Lembaga Ilmu Pengetahuan Indonesia, Jakarta: LIPI Press.

Reinking, A.O. & Wollenweber, H.W., 1927. Tropical Fusaria. The Philippine Journal of Science, 32(2), pp.104–244.

Salacinas, M., 2019. Spot On: Managing Panama Disease of Banana in The Philippines. Wegeningen University: The Netherlands.

Sandoval-Denis, M., Lombard, L. & Crous, P.W., 2019. Back to the roots: a reappraisal of Neocosmospora. Persoonia, 43(96), pp.90-185. doi: 10.3767/persoonia.2019.43.04

Seifert, K. et al., 2011. The Genera of Hyphomycetes, CBS biodiversity series No.9. Utrecht, The Netherlands: CBS-KNAW Fungal Biodiversity Center.

Valmayor, R., Jamaluddin, S.H. & Silahoy, J.S.B., 1999. Banana cultivar names and synonyms in Southeast Asia, INIBAP Montpeliier France.

Westerhoven, A.C.V., et al. 2022. Dissemination of Fusarium wilt of banana in Mozambique caused by Fusarium odoratissimum Tropical Race 4. Plant Disease. doi: 10.1094/PDIS-07-22-1576-SC.

White, T.J. et al., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols: A guide to methods and applications. New York: Academic Press, pp.315–322.

Zakaria, L. & Rahman, N.H.A., 2011. Endophytic Fusarium spp. from wild banana (Musa acuminata) roots. African Journal of Microbiology Research, 5(21), pp.3600-3602.

Zakaria, L. & Ning, C.H., 2013. Endophytic Fusarium spp. from Roots of Lawn Grass (Axonopus compressus). Trop Life sci Res., 24(2), pp.85–90. PMCID: PMC3935371

Zakaria, L. & Wan Aziz, W.N., 2018. Molecular identification of Endophytic Fungi from Banana Leaves (Musa spp.). Trop Life sci Res., 29(2), pp.201–211. doi: 10.21315/tlsr2018.29.2.14

Zhang, J., Chen, J. & Jia, R.M., 2018. Suppression of plant wilt diseases by nonpathogenic Fusarium oxysporum Fo47 combined with actinomycete strains. Biocontrol Sci Technol., 28(6), pp.562– 573. doi: 10.1080/09583157.2018.1468996.

Zheng, L.S.et al. 2012. The Fusarium species isolated from banana and their phylogenetic relationships. Mycosystema, 32(4), 617–632.

DOI: https://doi.org/10.22146/jtbb.76897