Effectivity of Spodoptera littoralis Nucleopolyhedrovirus (SpliMNPV) and Natural Additives Mixtures against Spodoptera litura Fab. (Lepidoptera: Noctuidae) on Cabbage Plants

https://doi.org/10.22146/jtbb.71134

Sukirno Sukirno(1*), Bellia Alda Ayu Prasetya(2), Aryo Seto Pandu(3), Siti Sumarmi(4), Hari Purwanto(5), Ignatius Sudaryadi(6), Suparmin Suparmin(7), RC Hidayat Soesilohadi(8)

(1) Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan Sekip Utara Depok 55281 Sleman Yogyakarta, Indonesia
(2) Undergraduate Student Faculty of Biology, Universitas Gadjah Mada, Indonesia
(3) Undergraduate Student Faculty of Biology, Universitas Gadjah Mada, Indonesia
(4) Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan Sekip Utara Depok 55281 Sleman Yogyakarta, Indonesia
(5) Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan Sekip Utara Depok 55281 Sleman Yogyakarta, Indonesia
(6) Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan Sekip Utara Depok 55281 Sleman Yogyakarta, Indonesia
(7) Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan Sekip Utara Depok 55281 Sleman Yogyakarta, Indonesia
(8) Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan Sekip Utara Depok 55281 Sleman Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


Armyworm (Spodoptera litura Fab.) is one of the agricultural pests that can cause huge losses especially for Indonesian farmers because it is damaging various crops, especially cabbage (Brassica oleifera L.). Spodoptera littoralis nucleopolyhedrovirus (SpliMNPV) is one of the biological agent which is effective for the management of the Spodoptera litura. However, because of UV radiation it easily degraded when applied in the fields. This study was aimed to determine the effectivity of several indigenous plants for UV protectant of SpliMNPV for controlling armyworm at greenhouse scale. Extracts of 2% (w/v) of turmeric rhizome, red betel leaf, moringa leaf, and clove flower, were formulated with SpliMNPV and sprayed evenly onto two-month- old cabbages. The experiment used five replicates with six periods of sunlight exposures (0, 1, 3, 5, 7, and 15 days). A commercial product of deltamethrin was used as a comparison. The sprayed leaves were then used as a bioassay by using 25 individuals of one day old 1st larval instar by five replicates. The results showed that the turmeric additive was the most effective as a UV protectant and effectively prolonged the half-life of SpliMNPV to 4.12 days, while for clove, moringa leaf, and red betel was 2.48, 2.15, and 2.28 days, respectively.


Keywords


SpliMNPV; Bioinsecticide; Brassica oleraceae L.; UV radiation

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References

Armenta et al., 2003. Impact of a nucleopolyhedrovirus bioinsecticide and selected synthetic insecticides on the abundance of insect natural enemies on maize in southern Mexico. J Econ Entomol., 96(3), pp.649-661. doi:10.1093/jee/96.3.649.

Bambal, V. & Mishra, M., 2014. Evaluation of in vitro sunscreen activity of herbal cream containing extract of Curcuma longa and Butea monosperma. World J Pharm Res., 3(2), pp.3026-3035. ISSN 2277 – 7105.

Bayer Crop Science, 2021. ‘Decis®’. Viewed 29th May 2021 from https://www.crop.bayer.com.au/find-crop-solutions/by-product/insecticides/decis-options-insecticide.

Cress, D., 1990. Factors Affecting Pesticide Behavior and Breakdown, Kansas State University.

Engelhard, E. K., & Volkman, L. E., 1995. Developmental resistance in fourth instar Tricholupsia ni orally inoculated with Autographa californica M. nuclear polyhe-drosis virus. J Virol., 209, pp.381- 389.

European and Mediterranean Plant Protection Organization (EPPO), 2015. Spodoptera littoralis, Spodoptera litura, Spodoptera frugiperda, Spodoptera eridania. EPPO Bull., 45(3), pp.410-444. doi: 10.1111/epp.12258.

Garad, G. P., Shivpuje, P. R. & Bilapate, G. G., 1984. Life fecundity tables of Spodoptera litura (Fabricius) on different hosts. Proc Animal Sci, 93(1), pp.29-33.

Jha et al., 2015. Effect of lyophilization on infectivity and viral load of adenovirus. Nepal J Biotech.. 3(1), pp.15-21. ISSN 2467-9319.

Khan et al., 2010. Phytochemical and biological investigation of Curcuma longa. J Pharm Sci., 8(1), pp.39-45. doi:10.3329/dujps.v8i1.5334

Lehane, M. J., 1997. Peritrophic matrix structure and function. Ann Rev Entomol., (42), pp.525-550.

Listorti, J. A. & Doumani, F. M., 2001. Environmental health: bridging the gaps, Washington: The World Bank Publications, pp.122-124.

Lynn, D. E., & Harrison, R. L., 2016. Routine maintenance and storage of lepidopteran insect cell lines and baculoviruses. In Baculovirus and insect cell expression protocols. Methods in molecular biology, Vol. 1350. Humana Press, New York. doi: 10.1007/978-1-4939-3043-2_9.

McPartland et al., 2000. Hemp diseases and pests: Management and biological control, London: CABI Publishing, p.57.

National Pesticide Information Center (NPIC), 2012. ‘Deltamethrin’. Viewed 29 May 2021 from http://npic.orst.edu/ingred/deltamethrin.html.

Prasetya, B. A. A. 2021. Patogenisitas Spodoptera littoralis nucleopolyhedrovirus (SpliMNPV) dan bahan aditif alami terhadap hama ulat grayak (Spodoptera litura Fab.) (Lepidoptera: Noctuidae) pada tanaman kubis (Brassica oleraceae L.), Universitas Gadjah Mada. (in Indonesian).

Reddy, P. P., 2015. Plant protection in tropical root and tuber crops. New Delhi: Springer, p.155.

Svobodová et al., 2003. Natural phenolics in the prevention of UV-induced skin damage. A review. Biomed Papers Med Fac Univ Palacky Olomouc Czech Rep., 147(2). pp.137-45.

Sisa, M. et al., 2010. Review: Photochemistry of Flvonoids. Molecules, 15, 5196-5245. doi:10.3390/molecules15085196.

Sukirno et al., 2018. The efficacy and persistence of Spodoptera littoralis nucleopolyhedrovirus (SpliMNPV) applied in UV protectants against the beet armyworm, Spodoptera exigua (Hübner)(Lepidoptera: Noctuidae) under Saudi field conditions. Pak J Zool., 50(5), pp.1895-1902.

Sukirno et al., 2019. The effectivity of Indonesian indigenous UV Protectant to enhance the pathogenicity and persistence of bio-insecticides for controlling army worm (Spodoptera litura Fab.). Project Report of PDUPT 2018. (in Indonesian).

Sutanto et al., 2017. Evaluation of natural additives to enhance the persistence of Spodoptera littoralis (Lepidoptera: Noctuidae) nucleopolyhedrovirus (SpliMNPV) under field conditions in Saudi Arabia. J Econ Entomol., 110(3), pp.924-930.

Vialard et al., 1995. Introduction to the molecular biology of baculoviruses. Methods Mol Biol., 39, pp.1–24. doi:10.1385/0-89603-272-8:1.



DOI: https://doi.org/10.22146/jtbb.71134

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