Neither Coral- nor Symbiont- Genetic Diversity may Explain the Resistance of the Coral Echinopora lamellosa to Bleaching

https://doi.org/10.22146/jtbb.66161

Imam Bachtiar(1*), Muhammad Irsyad Abiyusfi Ghafari(2), Ibadur Rahman(3), Baiq Hilda Astriana(4)

(1) Department of Mathematics and Science Education, FKIP, University of Mataram
(2) Department of Biology, Postgraduate School, Hasanuddin University
(3) Department of Marine Science, Faculty of Agriculture, University of Mataram
(4) Department of Aquaculture, Faculty of Agriculture, University of Mataram
(*) Corresponding Author

Abstract


Genetic diversity has an important role in the stability of coral populations in coping with disturbances. In the last three bleaching events, the coral Echinopora lamellosa survived better in the eastern- than the western- Lombok waters that are not related to algal symbiont diversity. The present study aimed to assess the genetic diversity of E. lamellosa from the two locations in the Lombok waters. The ITS1-5.8S-ITS2 (whole ITS region) marker was used to identify and to determine the genetic structure, genetic variation, and demographic pattern of E. lamellosa. The results showed that E. lamellosa of the two locations are two different populations. The haplotype diversity was very high indicating a predominance of sexual reproduction mode for both eastern and western populations. The phylogenetic topology suggests there is possible connectivity between populations, whereas the haplotype network exhibits a restricted gene flow between the two populations.  The results suggest that the present E. lamellosa populations were from both surviving colonies and new recruitment of long-distance larvae. Both population likely shares the same larvae supply brought from source-reefs in the Flores Sea or Makassar Strait by the Indonesian Throughflow. The present and previous studies revealed that genetic diversity alone yet to explain the resistance of E. lamellosa in eastern and western Lombok waters.

 

 

 


Keywords


bleaching; larval dispersal; population; re-colonization; survivorship

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References

Afiq-Rosli, L. et al., 2019. Maximising genetic diversity during coral transplantation from a highly impacted source reef. Conservation Genetics, 20(3), pp.629–637.

Aurelle, D. et al., 2020. Genetic insights into recolonization processes of Mediterranean octocorals. Marine Biology, 167(73).

Babcock, R.C. et al., 1994. Mass spawning of corals on a high latitude coral reef. Coral Reefs, 13, pp.161–169.

Bachtiar, I., 2001a. Promoting recruitment of scleractinian corals using artificial substrate in the Gill Indah, Lombok Barat, Indonesia. Proceeding 9th International Coral Reef Symposium Bali, 1, pp.425–430.

Bachtiar, I., 2001b. Reproduction of three scleractinian corals (Acropora cytherea, A. nobilis, Hydnophora rigida) in eastern Lombok Strait, Indonesia. Ilmu Kelautan, 21, pp.18–27.

Bachtiar, I. et al., 2016. Monitoring of Coral Reef Health and Related Ecosystems in Sekotong, Lombok Barat (in Indonesian language). Technical Report for COREMAP-CTI. Jakarta: Lembaga Ilmu Pengetahuan Indonesia. p.95.

Bachtiar, I. & Hadi, T.A., 2019. Differential impacts of 2016 coral bleaching on coral reef benthic communities at the Sekotong Bay, Lombok Barat, Indonesia. Biodiversitas, 20(2), pp.570–575.

Bachtiar, I. et al., 2019. Coral Echinopora lamellosa hosts multiple clades of symbionts in western Alas Strait, Indonesia. AIP Conference Proceedings, 2199, 070013.

Bandelt, H.J. et al., 1999. Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution, 16(1), pp.37–48.

Boilard, A. et al., 2020. Defining coral bleaching as a microbial dysbiosis within the coral holobiont. Microorganisms, 8(11), 1682.

Boulay, J.N. et al., 2012. High genotypic diversity of the reef-building coral Porites lobata (Scleractinia: Poritidae) in Isla del Coco National Park, Costa Rica. Revista de Biología Tropical, 60(3), pp.279–292.

Bourne, D.G. et al., 2016. Insights into the coral microbiome: Underpinning the health and resilience of reef ecosystems. Annual Review of Microbiology, 70, pp.317–340.

Brown, B.E. & Suharsono, 1990. Damage and recovery of coral reefs affected by El Niño related seawater warming in the Thousand Islands, Indonesia. Coral Reefs, 8, pp.163–170.

Buddemeier, R.W. & Fautin, D.G., 1993. Coral bleaching as an adaptive mechanism: A testable hypothesis. BioScience, 43(5), pp.320–326.

Camp, E.F. et al., 2016. Mangrove and seagrass beds provide different biogeochemical services for corals threatened by climate change. Frontiers in Marine Science, 3, 52.

Conti-Jerpe, I.E. et al., 2020. Trophic strategy and bleaching resistance in reef-building corals. Science Advances, 6(15), eaaz5443.

Cooke, I. et al., 2020. Genomic signatures in the coral holobiont reveal host adaptations driven by Holocene climate change and reef specific symbionts. Science Advances, 6(48), eabc6318.

Excoffier, L. & Lischer, H.L.L., 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10(3), pp.564–567.

Excoffier, L. et al., 1992. Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics, 131(2), pp.479–491.

Fuller, Z.L. et al., 2020. Population genetics of the coral Acropora millepora: Toward genomic prediction of bleaching. Science, 369(6501), eaba4674.

Gardes, M. & Bruns, T.D., 1993. ITS primers with enhanced specificity for Basidiomycetes - Application to the identification of mycorrhizae and rusts. Molecular Ecology, 2(2), pp.113–118.

Gomez, E. J. et al., 2018. Gametogenesis and reproductive pattern of the reef-building coral Acropora millepora in northwestern Philippines. Invertebrate Reproduction & Development, 62(4), pp.202-208.

Hemond, E.M. & Vollmer, S.V., 2010. Genetic diversity and connectivity in the threatened staghorn coral (Acropora cervicornis) in Florida. PLos One, 5(1), e8652.

Huang, W. et al., 2018. Genetic diversity and large-scale connectivity of the scleractinian coral Porites lutea in the South China Sea. Coral Reefs, 37(4), pp.1259–1271.

Hughes, T.P. et al., 2018. Spatial and temporal patterns of mass bleaching of corals in the Anthropocene. Science, 359(6371), pp.80-83.

Jompa, J. et al., 2020. Genetic patterns of the corals Euphyllia glabrescens and Lobophyllia corymbosa across the Indonesian Archipelago. Biodiversitas, 21(6), pp.2492–2499.

Kellogg, C.A. et al., 2020. Identifying mangrove-coral habitats in the Florida Keys. PeerJ, 8(1), e9776.

Kenkel, C.D. & Bay, L.K., 2018. Exploring mechanisms that affect coral cooperation: Symbiont transmission mode, cell density and community composition. PeerJ, 6(1648), e6047.

Leigh, J.W. & Bryant, D., 2015. PopART: Full-feature software for haplotype network construction. Methods in Ecology and Evolution, 6(9), pp.1110–1116.

Librado, P. & Rozas, J., 2009. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11), pp.1451–1452.

López-Portillo, J. et al., 2017. Water quality and mangrove-derived tannins in four coastal lagoons from the Gulf of Mexico with variable hydrologic dynamics. Journal of Coastal Research, 77, pp.28-38.

Loram, J.E. et al., 2007. Molecular quantification of symbiotic dinoflagellate algae of the genus Symbiodinium. The Biological Bulletin, 21(3), pp.259-268.

Lord, S.C. et al., 2020. Multi-year viability of a reef coral population living on mangrove roots suggests an important role for mangroves in the broader habitat mosaic of corals. Frontiers in Marine Science, 7, p.377.

Magalon, H., Adjeroud, M. & Veuille, M., 2005. Patterns of genetic variation do not correlate with geographical distance in the reef-building coral Pocillopora meandrina in the South Pacific. Molecular Ecology, 14(7), pp.1861–1868.

Marshall, P.A. & Baird, A.H., 2000. Bleaching of corals on the Great Barrier Reef: Differential susceptibilities among taxa. Coral Reefs, 19(2), pp.155-163.

Miller, K.J. & Ayre, D.J., 2004. The role of sexual and asexual reproduction in structuring high latitude populations of the reef coral Pocillopora damicornis. Heredity, 92, pp.557–568.

Monismith, S.G. et al., 2018. Transport between Palau and the Eastern Coral Triangle: Larval connectivity or near misses. Geophysical Research Letters, 45(10), pp.4974-4981.

Morikawa, M.K. & Palumbi, S.R., 2019. Using naturally occurring climate resilient corals to construct bleaching-resistant nurseries. Proceedings of the National Academy of Sciences of the United States of America, 116(21), pp.10586-10591.

Nakajima, Y. et al., 2010. Gene flow and genetic diversity of a broadcast-spawning coral in northern peripheral populations. PLos One, 5(6), e11149.

Noreen, A.M. et al., 2009. Genetic diversity and connectivity in a brooding reef coral at the limit of its distribution. Proceedings of the Royal Society B: Biological Sciences, 276(1675), pp.3927–3935.

Peixoto, R.S. et al., 2017. Beneficial microorganisms for corals (BMC): Proposed mechanisms for coral health and resilience. Frontiers in Microbiology, 8, 341.

Peluso, L. et al., 2018. Contemporary and historical oceanographic processes explain genetic connectivity in a Southwestern Atlantic coral. Scientific Reports, 8, 2684.

Petersen, D. et al., 2007. Sexual reproduction of scleractinian corals in public aquariums: current status and future perspectives. International Zoo Yearbook, 41(1), pp.122–137.

Richards, Z.T. & van Oppen, M.J., 2012. Rarity and genetic diversity in Indo–Pacific Acropora corals. Ecology and Evolution, 2(8), pp.1867-1888.

Rosser, N.L. et al., 2020. Geography and spawning season drive genetic divergence among populations of the hard coral Acropora tenuis from Indonesia and Western Australia. Coral Reefs, 39(1), pp.989–999.

Schweinsberg, M. et al., 2017. Inter‐and intra‐colonial genotypic diversity in hermatypic hydrozoans of the family Milleporidae. Marine Ecology, 38(1), e12388.

Smallhorn-West, P.F. et al., 2020. Coral reef annihilation, persistence and recovery at Earth’s youngest volcanic island. Coral Reefs, 39, pp.529–536.

Sprintall, J. et al., 2009. Direct estimates of the Indonesian Throughflow entering the Indian Ocean: 2004-2006. Journal of Geophysical Research, 114(C7), C07001.

Starger, C.J. et al., 2010. The recovery of coral genetic diversity in the Sunda Strait following the 1883 eruption of Krakatau. Coral Reefs, 29(3), pp.547–565.

Stewart, H.A. et al., 2021. Caribbean mangrove forests act as coral refugia by reducing light stress and increasing coral richness. Ecosphere, 12(3), e03413.

Swain, T.D. et al., 2021. A phylogeny-informed analysis of the global coral-symbiodiniaceae interaction network reveals that traits correlated with thermal bleaching are specific to symbiont transmission mode. mSystems, 6(3), e00266–21.

Takabayashi, M. et al., 2003. Genetic variation of the scleractinian coral Stylophora pistillata from western Pacific reefs. Coral Reefs, 22(1), pp.17–22.

Tamura, K. et al., 2013. MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30(12), pp.2725–2729.

Terrana, L. et al., 2021. ITS1 variation among Stichopathes cf. maldivensis (Hexacorallia: Antipatharia) whip black corals unveils conspecificity and population connectivity at local and global scales across the Indo-Pacific. Coral Reefs, 40(1), pp.521–533.

Wall, C.B. et al., 2020. Divergent symbiont communities determine the physiology and nutrition of a reef coral across a light-availability gradient. The ISME Journal, 14, pp.945–958.

Wijayanti, D.P. et al., 2018. Molecular identification and genetic diversity of Acropora hyacinthus from Boo and Deer Island, Raja Ampat, West Papua. IOP Conference Series: Earth and Environmental Science, 116, 012065.

Yusuf, S. et al., 2013. Reproduction pattern and multispecific spawning of Acopora spp. in Spermonde Islands reefs, Indonesia. Ilmu Kelautan: Indonesian Journal of Marine Sciences, 18, pp.172-178.

Zamani, N.P. et al., 2019. The intracellular photopigment and glutathione (GSH) dynamics in symbiodinium natural population during light stress and recovery. IOP Conference Series: Earth and Environmental Science, 325, 012015.



DOI: https://doi.org/10.22146/jtbb.66161

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