Screening and Identification of Mushrooms Growth Promoting Bacteria on Straw Mushrooms (Volvariella volvacea)
Indah Juwita Sari(1*), I Nyoman Pugeg Aryantha(2)
(1) Department of Biology Education, Faculty of Teacher Training and Education, Universitas Sultan Ageng Tirtayasa, Serang
(2) Department of Biology, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung
(*) Corresponding Author
Abstract
This research aimed to identify the indigenous Mushroom Growth Promoting Bacteria (MGPB) bacteria that can increase the growth of Volvariella volvacea. The research began by isolating indigenous MGPB from planting media of straw mushrooms in Karawang, Indonesia. The screening was performed to select bacterial isolates that can promote the highest growth of mushrooms by dual culture method on PDA media. There were 10 of the 58 highest bacterial isolates that have a positive effect on the vegetative growth of mushrooms. The 23K bacterial isolate was the most significant increase in mycelium growth compared to other isolates and bacteria-induced controls. A bacterial isolate 25K by gene analysis was identified by 16S rRNA (518F primer (5’- CCA-GCA-GCC-GCG-GTA-ATA-CG -3’) and 800R primer (5’- TAC-CAG-GGT-ATC-TAA-TCC -3’). The result from gene analysis shows that there are ~1550 base pairs products. BLAST analysis and phylogenetic tree adjustment results show that the closest diversity of this bacterial isolate 25K is Bacillus thuringiensis serovar konkukian str. 92-27 (equality value = 99%).
Keywords
Full Text:
PDFReferences
Araujo, W.L., W. Maccheroni Jr., C.I Aguilar-Viloso, P.A.V. Barroso, H.O. Saridakis, and Azevedo, J.L. 2001. Variability and Interactions Between Endophytic Bacteria and Fungi Isolated from Leaf Tissue of Citrus Rootstocks. Can. J. Microbiol. 47; 229-236.
Baker, G.C., Smith, J.J., and Cowan, D.A. 2003: Review and Re - Analiysis of Dominan Spesific 16S primers. Journal of Microbiology, 55; 541 – 555.
Bernhard, K., and Utz, R. 1993. Production of Bacillus thuringiensis insecticides for eksperimental and commerercial Uses, 255-265.
Cappuccino, J.G. and Sherman, N. 2005. Microbiology A Laboratory Manual: Seventh Edition. State University of New York
Chang, S.T., and Miles, P.G. 2004: Edible Mushrooms and Their Cultivation, Academic Press, London, 345.
Clarridge, J.E. (2004). Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. CLINICAL MICROBIOLOGY REVIEWS. Vol. 17, No. 4. p. 840–862.
Darwis, A.A., Syamsu, K., and Salamah, U. 2004. Kajian produksi bioinsektisida dari Bacillus thuringiensis sub sp israelensis pada media tapioka. Jurnal teknologi industri pertanian. 14(1);1-5.
Dharmayanti, N.L.P.I. 2011. Filogenetika Molekuler: Metode Taksonomi Organisme Berdasarkan Sejarah Evolusi. Wartazoa. 21(1); 1-10.
El-kersh, T.A., Al-sheikh, Y.A., Al-akeel, R.A., and Alsayed, A. 2012. Isolation and characterization of native Bacillus thuringiensis isolates from Saudi Arabia. African journal of Biotechnology. 11(8); 1924-1938.
Familoni, TV., C.O. Ogidi., B.J. Akinyele., A.K. Onifade. 2018. Genetic Diversity, Microbial Study and Composition of Soil Associated with wild pleurotus ostreatus from Different Locations in Ondo and Ekiti States, Nigeria. Chemical and Biological Technologies in Agriculture. 5(7):1-12.
Haq, I.U., Khan, M.A., Khan, S.S., and Ahmad, M. 2011. Biochemical analysis of fruiting bodies of Volvariella volvacea strain Vv pk grown on six different substrate. Soil Environ. 30(2); 146-150.
Hatmanti, A. 2000. Pengenalan Bacillus SPP. Oseana. 25(1); 31-41.
Pakpahan, M., Ekowati, C.N., and Handayani, K. 2013. Karakterisasi fisiologi dan pertumbuhan isolat bakteri Bacillus thuringiensis dari tanah naungan lingkungan Universitas Lampung. Seminar Nasional Sains & Teknologi V lembaga penelitian Universitas Lampung. 751-759.
Payapanon, A., Suthirawut, S., Shompoosang, S., Tsuchiya, K., Furuya, N., Roongrawee, P., Kulpiyawat, T., and Somrith, A. (2011): Increase in Yield of the Straw Mushroom (Volvariella volvacea) by Supplement with Paenabacillus and Bacillus to the Compost. Journal Faculty of Agariculture, Kyushu University. 56 (2), 249-254.
Pion, M., Spangenberg, J.E., Simon, A., Bindschedler, S., Flury, C., Chatelain, A., Bshary, R., Job, D., and Junier, P. 2013. Bacterial farming by the fungus Morchella crassipes. Proceedings of the Royal Society, Biological Science; 1-9.
Roh, Yul, J., Choi, J.Y., Li, M.S., Jin, B.R., and Je, Y.H. 2007. Bacillus thuringiensis as a Specific, Safe, and Effective Tool for Insect Pest Control. Journal Microbiol Biotechnol.17(4); 547–559.
Sukendro, L., A. W. Gunawan, and O. S. W. Dharmaputra. 2001. Pengaruh Waktu Pengomposan Limbah Kapas terhadap Produksi Jamur Merang. Jurnal Mikrobiologi Indonesia. 6 (1): 19-22.
Young, L.S., Chu, J.N., Hameed, A., and Young, C.C. 2013: Cultivable Mushroom Grwoth Promoting Bacteria and Their Impact on Agaricus blazei Productivity, Pesquisa Agropecuária Brasileira, 48(6), 636 - 644.
Yuwono, T. 2006. Teori dan Aplikasi Polymerase Chain Reaction. Penerbit Andi. Yogyakarta.
Zeranejad, F., Yakhchali, B., and Rasooli, I. 2012: Evaluation of Indigenous Potent Mushroom Growth Promoting Bacteria (MGPB) on Agaricus bisporus Production. Journal Microbiol Biotechnol, 28, 99 – 104.
DOI: https://doi.org/10.22146/jtbb.60618
Article Metrics
Abstract views : 3551 | views : 2555Refbacks
- There are currently no refbacks.
Copyright (c) 2021 Journal of Tropical Biodiversity and Biotechnology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Editoral address:
Faculty of Biology, UGM
Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ISSN: 2540-9581 (online)