Potential Development of Purwoceng (Pimpinella pruatjan Molk or Pimpinella alpine Kds) Plant Scale Industry Using In-Vitro Culture Technique By Means of Rooting Induction

https://doi.org/10.22146/jtbb.38849

Egi Nuryadin(1*), Alyaa Nabiila(2)

(1) Department of Biology Education, Faculty of Teacher Training and Education, Siliwangi University
(2) Department of Biology Education, Faculty of Teacher Training and Education, Siliwangi University
(*) Corresponding Author

Abstract


Purwoceng (Pimpinella pruatjan Molk or Pimpinella alpine KDS) belongs to the Apiaceae family, is an endemic plant in mountainous areas such as the Dieng Plateau, Pangrango Mountain, and mountainous areas in East Java. Parts of the plants, especially roots are efficacious as aphrodisiacs, diuretic drugs and tonics. Based on CITES, this plant belongs to the category of endangered species, so its presence is very rare in nature. This scarcity occurs due to the purwoceng exploitation in nature as traditional herbal medicine without any cultivation or rejuvenation. In situ conservation efforts are almost impossible because the original habitat of this plant has become extinct. Thus, ex situ conservation is more suitable to be applied, by using in-vitro culture techniques. Therefore, a research study is needed on the development of tissue culture techniques in the production of the seeds. Methods of data collection in this study was conducted by literature studies. Therefore, ex-vitro root induction techniques are applied currently, namely rooting induction. The content of the chemical compounds and various properties make purwoceng as a commodity in the raw materials of drugs that have great potential to promote industrial players in the economic field. Based on the results from the literature, the authors can conclude that the potential of purwoceng plants is quite large, but still constrained by the scarcity of seed supply so the application of ex-vitro root induction techniques in in-vitro culture can be a solution in limitation of supply of purwoceng crops for industrial scale.

 

Purwoceng (Pimpinella pruatjan Molk or Pimpinella alpine KDS) belongs to the Apiaceae family, is an endemic plant in mountainous areas such as the Dieng Plateau, Pangrango Mountain, and mountainous areas in East Java. Parts of the plants, especially roots are efficacious as aphrodisiacs, diuretic drugs and tonics. Based on CITES, this plant belongs to the category of endangered species, so its presence is very rare in nature. This scarcity occurs due to the purwoceng exploitation in nature as traditional herbal medicine without any cultivation or rejuvenation. In situ conservation efforts are almost impossible because the original habitat of this plant has become extinct. Thus, ex situ conservation is more suitable to be applied, by using in-vitro culture techniques. Therefore, a research study is needed on the development of tissue culture techniques in the production of the seeds. Methods of data collection in this study was conducted by literature studies. Therefore, ex-vitro root induction techniques are applied currently, namely rooting induction. The content of the chemical compounds and various properties make purwoceng as a commodity in the raw materials of drugs that have great potential to promote industrial players in the economic field. Based on the results from the literature, the authors can conclude that the potential of purwoceng plants is quite large, but still constrained by the scarcity of seed supply so the application of ex-vitro root induction techniques in in-vitro culture can be a solution in limitation of supply of purwoceng crops for industrial scale.

 


Keywords


Purwoceng; in-vitro culture; rooting induction

Full Text:

PDF


References

Darwati, I., & Roostika, I. (2006). Status Penelitian Purwoceng (Pimpinella alpina Molk.) di Indonesia. Buletin Plasma Nutfah, 9-15.

Ditjen POM. 2000. Kebijakan nasional pengembangan obat tradisional. Departemen Kesehatan RI Jakarta, 68p.

Ermayanti, TM., D.R Wulandari, A.,F,Martin.,E Al Hafiz,D. E Rantau & B.W.,Haspari. (2006). Pengembangan Teknik In Vitro Untuk Perbanyakan Bibit Unggul Tanaman Purwoceng (Pimpinla Alpina (Zoll).Koord). Http;//www.elib.pdii.lipi.go.id. Diakses pada tanggal 16/12/2012.

Hernani, Rostiana O., 2004, 'Analisis kimia akar purwoceng (Pimpinella pruatjan) 'Di dalam: Indonesian Biopharmaca and Excibition and Conference. Proceedings of the Facilitation of the Biopharmaceutical Development Cooperation Forum, Yogyakarta, July 14-15, 2004.

Mariani, Totik Sri. (2017). Meristem Culture and its Stigmasterol Content of Purwoceng (Pimpinella pruatjan Molk.) for Agricultural Sciences. International Journal of Innovations in Engineering and Technology (IJIET), 250-253.

Meiny. 2017. Effect of Purwoceng Extracts (Pimpinella Alpina Molk) on Erectile Function Biomarker Enhancement. PJMHS, 1182-1187.

Rahardjo, M., 2003, 'Purwoceng tanaman obat aprodisiak yang langka'. Warta Penelitian dan Pengembangan Tanaman Industri, 9 :4-7.

Rahardjo M, Wahyuni S, Trisilawati O dan Djauhariya E. 2005. Ciri agronomis, mutu dan lingkungan tumbuh Tanaman obat langka purwoceng (pimpinella Pruatjan molk.). Prosiding seminar nasional tumbuhan obat indonesia xxviii – bogor, 15 – 16 september 2005.

Rahardjo, M. & Darwati, I., 2006, 'Produksi dan mutu simplisia purwoceng berdasarkan lingkungan tumbuh dan umur tanaman', paper presented at POKJANAS TOI XXVIII conference, Agriculture Department, Bogor, September 15–16.

Rao, C. K., Geetha, B. L., & Suresh, G. (2003). Red List of Threatened Vascular Plant Species in India. Bangalore: Envis.

Rivai, M.A., Rugayah, and E.A. Widjaja. 1992. Thirty years of the eroded species

medicinalcrops. Floribunda. Pioneer of Indonesian Plant Taxonomy, Bogor. 28 p.Research Institute for Medicinal and Aromatic Plants, n.d, Standar Operasional Prosedur (SOP) Purwoceng (Pimpinella pruatjan Molkenb), perkebunan.litbang.pertanian.go.id, viewed 22 June 2018, from https://perkebunan.litbang.pertanian.go.id/wp-content/uploads/2017/03/sop-purwoceng.pdf

Roostika, I., darwati., Magia R. 2007. Kriopreservasitanaman Purwoceng {Pimpinella pruatjan Molk.) Dengan Teknik Vitrifikasi. Berita Biologi 8(6).

Roostika, I., Purwaningsih, R., & Darwati, I. (2009). Penyimpanan In Vitro Tanaman Purwoceng (Pimpinella pruatjan Molk.) Melalu Aplikasi Pengenceran Media dan Paclobutrazol. LITTRI, 84-90.

Rostiana, O. 2014. Pruatjan (Pimpinella pruatjan Molk.): The rooted herbal medicine of Indonesia for aphrodisiac properties. 2nd International Conference and Exhibition on Pharmacognosy, Phytochemistry & Natural Products. August 25-27, 2014 DoubleTree by Hilton Beijing, China.

Rusmin, Devi. 2017. Pengembangan Budidaya Purwoceng (Pimpinella pruatjanMolk.) sebagai Tanaman Obat. Perspektif Vol. 16 No. 2 /Des 2017. Pp 80-93

Sidik, Sasongko, E. Kurnia, dan Ursula. 1975. Usaha isolasi turunan kumarin dari akar purwoceng (Pimpinella alpina Molk.) asal dataran tinggi Dieng. Dalam Simposium Tanaman Obat I, 8-9 Desember, Bagian Farmakologi. FKH, Institut Pertanian Bogor.

Suzery, M., B. Cahyono, Ngadiwiyana, dan H. Nurhasnawati. 2004. Senyawa stigmasterol dari Pimpinella alpina Molk. Suplemen 39(1):39-41.

Syahid, S.F., O. Rostiana, dan Miftakhurohmah, 2004. Pengaruh NAA dan IBA terhadap Perakaran Purwoceng (Pimpinella pruatjan). Prosiding Fasilitasi Forum Kerjasama Pengembangan Biofarmaca, IBEC. 14 – 18 Juli 2004. Yogyakarta.

Suzery, M., Cahyono, B. & Ngadiwiyana, Nurhanawati H, 2004, 'Senyawa stigmasterol dari impinella alpine Molk. (Purwoceng) ', Suplemen 39(1), 39-41.

Taufiqqurrachman., & Wibowo., 2006, 'Effect of purwoceng (Pimpinella alpina) Extract in stimulating testosterone, luteinizing Hormone (LH) and follicle stimulating hormone (FSH) in Sprague Dawley male rats. Proceedings of the National Seminar on Indonesian Medicinal Plants XXVIII, Bogor, September 15-16, 2006

Wahyuningrum, e. a. (2016). Screening of Potential Free Radicals Scavanger and Antibacterial Activities of Purwoceng (Pimpinella alpina Molk). Tropical Life Sciences, 161-166.

Yuhono, J.T., 2004, 'Usahatani purwoceng (Pimpinella pruatjan Molkenb., potensi, peluang, dan masalah pengembangannya', Buletin Penelitian Tanaman Rempah dan Obat, 15(1), 25-32.



DOI: https://doi.org/10.22146/jtbb.38849

Article Metrics

Abstract views : 6727 | views : 4219

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Journal of Tropical Biodiversity and Biotechnology

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Editoral address:

Faculty of Biology, UGM

Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia

ISSN: 2540-9581 (online)