Kajian Keragaman Genetik Isolat Ralstonia solanacearum BIOVAR 3 menggunakan Penanda REP-PCR

https://doi.org/10.22146/jpti.9957

Yadi Suryadi(1*)

(1) Balai Penelitian Biogen-Bogor
(*) Corresponding Author

Abstract


Study of DNA fingerprinting of genomic DNA of Australian Ralstonia solanacearum biovar 3 was characterized by a DNA BOX primer that correspond with repetitive sequence using PCR amplification (rep-PCR). Based on rep-PCR DNA profiles different band mobility were observed among Australian biovar 3. Most of isolates have shown common DNA amplification product at 600 bp. Cluster analysis to the DNA profiles showed two different DNA banding patterns that correlated with geographical origins of the isolates. Subgroup A correspond well with isolates from South Queensland/New South Wales, whilst subgroup B correspond with isolates from North Queensland origin.


Keywords


Ralstonia solanacearum; rep-PCR; marker

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References

Buddenhagen, I.W & A. Kelman. 1964. Biological and physiological aspects of bacterial wilt caused by P. solanacearum. Annu. Rev. Phytopathol. 2:203-230.

Cook, D., E. Barlow, & L. Sequeira. 1989. Genetics diversity of P. solanacearum detection of RFLP with DNA probes that specify virulence and the hypersensitive response. Mol. Plant Microbe Interact. 2:203-230.

deBruijn, F.J. 1992. Use of rep (repetitive extragenic palindromic and enterobacterial repetitive intergenic consencus) sequences and the PCR to fingerprint the genomes of Rhizobium melilotti isolates and other soil bacteria. AppI. Environ. Microbiol., 58(7):2180-2187.

Frey, P., J. Smith., L. Albar., P. Prior., G.C Saddler., D. T. Demery, & A, Trigalet. 1996. Bacteriocyn typing of Burkholderia (Pseudomonas) solanacearum race 1 of the French West Indies and correlation with genomic variation of the pathogen. Appl. Environ. Microbiol. 62:473-479.

Hayward A.C. 1964. Characteristics of P. solanacearum. J. Appl. Bacteriol. 27:265- 277.

Hayward, A.C. 1991. Biology and epidemiology of Pseudomonas solanacearum. Annu. Rev. Phytopathol. 29:65-87.

Ito, S., T. Fujii., Y. Shijima S. Tanaka., M. Kanaya-Iwaki., S. Yoshiwara & F. Kishi. 1996. Genomic diversity of field isolates of B. solanacearum in Japan. J. Phytopathol. 144:504-505.

Louws, F.J., D.W Fulbright, C.T Stephens, & FJ. deBruijn. 1995. Differentiation of genomic structure by rep-PCR fingerprinting to rapidly classify Xanthomonas campestris pv. vesicatoria. Phytopathology 85(5):528-536.

Machmud, M. 1998. Menuju kesamaan persepsi terhadap taksonomi bakteri P. solanacearum (Smith, 1896) Smith 1914. Bull. Agrobio. 2 (l):16-21.

Opina, N., F. Tavner., G. Hallway., J.F. Wang, T.H Li, R. Maghirang., M. Fegan.,. A.C Hayward., V. Khrisnapillai., W.F Hong., B.W Holloway, & J.N Timmis. 1997. A novel method for development of species and strain- specific DNA probes and PCR primers for identifying B. solanacearum (formerly P. solanacearum ). Asia Pacific J. Mol. Biol. Biotechnol. 5:19-30.

Sambrook, J., E.F Fritsch, & T. Maniatis. 1989. Molecular cloning, a laboratory manual. Cold Spring Harbor, New York,

Schneider, M & F.J de Bruijn .1996. Rep PCR mediated genomic fingerprinting of Rhizobia and computer-assisted phylogenetic pattern analysis. World J. Microbiol. Biotechnol. 12:163-174.

Seal, S., L. Jackson, & M.J. Daniels. 1992. Development of molecular diagnostic technique for detection of P. solanacearum and identification of sub groups within this species In: Bacterial wilt (Hartman, G.L and A.C Hayward (eds). ACIAR Proc. no 45, Canberra, Australia.

Suryadi, Y., M.Fegan, & A.C Hayward. 1994. Penggunaan pelacak 16S rDNA untuk membedakan biovar P. solanacearum. Risalah Hasil Penel. Tan. Pangan. 1:42-51.

Van Damme, P., B.A.J, Gissendorf., A. Van Belkum, D. Pierrad., S Lauwers., K. Kersters., J.P Butzler., H Goosens & W.G.V Quint. 1993. Discrimination of epidemic and sporadic isolates of Acrobacter butzleri by PCR-mediated DNA fingerprinting. J. Clinic. Microbiol. 31(12):3317-33 19.

Versalovic, J., T. Koeth., & J.R. Lupski. 1991. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 19:6823-6831.

Yabuuchi, E., Y. Kosako., I, Yano., H. Hotta & N. Nishiuchi. 1995. Transfer of two Burkholderia and an alacaligenes species to Raistonia gen nov-proposal of R.pickettii (Ralston, Palleroni and Doudoroff, 1973) comb.nov, R. solanacearum (Smith, 1896) comb.nov, and R. eutropha (Davis, 1969) comb.nov. J. Microbiol. Immunol. 39(1 1):897-904.



DOI: https://doi.org/10.22146/jpti.9957

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