Analisis Variabilitas Genetik Rigidoporus microporus (Jamur Akar Putih) pada Karet dengan Teknik PCR-RFLP Fragment ITS

https://doi.org/10.22146/jpti.9383

Ria Novianti(1), Haryono Semangun(2), Ferry F. Karwur(3*), Martanto Martosupono(4)

(1) Magister Biologi Universitas Kristen Satya Wacana Salatiga
(2) Magister Biologi Universitas Kristen Satya Wacana Salatiga
(3) Magister Biologi Universitas Kristen Satya Wacana Salatiga
(4) Magister Biologi Universitas Kristen Satya Wacana Salatiga
(*) Corresponding Author

Abstract


The eleven isolates studied fell into two groups, which showed that the fungus did not have high genetic variability. Isolates from diseased rubber trees in shorter vicinities, which were due to pathogen’s vegetative spread through root contact, have close genetic relationship. Meanwhile isolates from far apart trees, either in one estate or from different islands, does not necessarily have distant genetic relationship. It might be due to the fact that long distance dispersal of the disease could also due to rhizomorph contaminating rubber stumps. PCR-RFLP Fragment ITS Techniques can be applied to study genetic relationship of Rigidoporus microporus isolates. It is expected that the technique will be applied with more isolates from different provinces by using additional different restriction enzymes.

Kesebelas isolat yang diteliti terbagi ke dalam dua kelompok, yang menunjukkan bahwa jamur ini tidak mempunyai variabilitas genetik yang tinggi. Isolat-isolat dari pohon karet sakit yang letaknya berdekatan, yang terjadi karena penularan vegetatif melalui kontak akar, mempunyai hubungan kekerabatan genetik yang erat. Isolat-isolat dari pohon karet sakit yang berjauhan, baik dalam satu kebun maupun dari pulau lain, tidak selalu mempunyai hubungan kekerabatan genetik yang jauh. Ini dapat disebabkan karena adanya penyebaran penyakit jarak jauh secara vegetatif dengan terbawanya rizomorf pada akar stump. Teknik PCR-RFLP Fragment ITS dapat dimanfaatkan untuk penelitian kekerabatan Rigidoporus microporus. Pemakaiannya perlu diperluas dengan pengumpulan isolat yang lebih tersebar, dengan memakai enzim restriksi yang lebih banyak.


Keywords


genetic variability; jamur akar putih karet; PCR-RFLP Fragment ITS Technique; Rigidoporus microporus; white root rot of rubber; variabilitas genetik

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DOI: https://doi.org/10.22146/jpti.9383

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